Large tissue slice embedding and clamping assembly

Abstract

The invention discloses a large tissue slice embedding and clamping assembly, and relates to the technical field of embedding boxes, the large tissue slice embedding and clamping assembly comprises a clamping adaptive seat and an embedding sheet, two parallel clamping surfaces are arranged on two sides of the clamping adaptive seat, the clamping surfaces are used for clamping a sample clamp of an automatic pathological paraffin embedding wheel type slicer, and the embedding sheet is arranged on the clamping adaptive seat. The tops of the two parallel clamping faces are connected through a positioning face, the end faces of the bottoms of the two parallel clamping faces are flush and can abut against the bottom face of the sample clamp, an embedding piece is detachably connected to the positioning face, and a large tissue slice wax block is arranged on the embedding piece. The clamping adaptive seat is fixedly connected with a sample clamp of a slicing machine, and a plurality of embedding pieces can be replaced for slicing through one-time clamping of the clamping adaptive seat, so that a large tissue slice wax block can be quickly clamped, the position of the rear embedding piece can be relatively fixed, the section angle can be kept on the same section after repeated slicing, and the slicing efficiency is greatly improved. And the phenomenon that the focus tissue is cut by repeatedly adjusting the angle of the tangent plane is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of embedding boxes, in particular to a large tissue slice embedding and clamping component. Background Art

[0002] Embedding is a technique for encapsulating a powdered or bulk structure with an embedding agent (such as organic plastics, metals, or paraffin wax) to provide physical support or chemical protection. This technique is widely used in pathology. In tissue section preparation, embedding primarily involves fixing dehydrated tissue with paraffin wax to form a hard block, facilitating subsequent cutting into thin sections (typically 4 μm) for staining and microscopic observation.

[0003] The wax blocks used for large tissue sections are designed to visualize the entire organ or tumor and its surrounding tissues on a single pathology slide. This allows for the identification of small lesions, calculation of tumor proportions, and the relationship between the tumor and surrounding tissues, as well as a comprehensive assessment of tumor infiltration. Currently, wax blocks for embedding large tissue sections can be up to 5.5 cm long, 4.8 cm wide, and over 1.5 cm thick (even up to 2 cm). The thickness of the wax blocks used for clamping alone is at least 5 mm, making them bulky and unable to be stored in conventional wax block cabinets. They must be archived separately, which is also bulky and expensive to store.

[0004] Existing large paraffin blocks for pathological tissue sections are typically held in a rotating sample clamp that rotates and secures the block with a nut when sliced ​​using an automatic paraffin embedding wheel microtome. Because the rotating clamping device (sample clamp) lacks a wax block retaining structure, and the conventional wax block embedding chuck is too small to be used for large pathological tissue section embedding, the sample clamp cannot be used to quickly and directly clamp the large paraffin blocks for pathological tissue sections. Consequently, the sample clamp must be manually adjusted repeatedly for section angle, requiring the sample clamp to be rotated multiple times. This repeated clamping of the sample clamp can also easily cause the paraffin block to break. If the sectioning angles differ when the paraffin block is moved horizontally or vertically, either the tissue section is incomplete or a significant amount of tissue may be removed to ensure a complete section. This is particularly true when re-sectioning is required to assess small lesions. This can lead to the removal of lesion tissue during the slicing process because the cross-section angle does not correspond to the previous section. Therefore, there is an urgent need to propose an embedding clamping assembly for large tissue sections to achieve rapid clamping and maintain the section at the same angle during repeated slicing. Summary of the Invention

[0005] The purpose of the present invention is to provide a large tissue section embedding clamping assembly to solve the problems existing in the above-mentioned prior art, so that large tissue section wax blocks can be quickly clamped and can be kept on the same angle of section during repeated slicing.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a large tissue section embedding clamping assembly, comprising a clamping adapter and an embedding sheet. Two parallel clamping surfaces are provided on both sides of the clamping adapter, and the clamping surfaces are used to clamp on a sample clamp of an automatic pathological paraffin embedding wheel slicer. The tops of the two parallel clamping surfaces are connected by a positioning surface, and the end surfaces of the bottoms are flush and can conflict with the bottom surface of the sample clamp. The embedding sheet is detachably connected to the positioning surface, and the embedding sheet is provided with a large tissue section wax block.

[0008] Preferably, the clamping adapter seat includes an oblique side surface, a step surface, the positioning surface and two clamping surfaces, the positioning surface is rectangular and is sequentially connected with the oblique side surface, one clamping surface, the step surface and another clamping surface on all four sides, and the oblique side surface, two clamping surfaces, the step surface and the positioning surface are spliced ​​to form a frame with a flat bottom and an open surface; the two clamping surfaces are connected to the long sides of the positioning surface.

[0009] Preferably, the material of the clamping adapter seat includes metal or metal alloy; the material of the embedding sheet includes polyester or polypropylene.

[0010] Preferably, the positioning surface is provided with a plug-in slot or a plug-in block, and the back side of the embedding sheet is provided with a plug-in block or a plug-in slot, and the plug-in block can be plugged into the plug-in slot.

[0011] Preferably, the plug-in slot and the plug-in block each include a pair of strip-shaped protrusions, the cross-section of the strip-shaped protrusions is L-shaped, T-shaped, a right-angled trapezoid or an isosceles trapezoid, and the two pairs of the strip-shaped protrusions can be mutually limited and clamped in a direction perpendicular to the strip-shaped protrusions and the positioning surface; the length direction of the strip-shaped protrusions is perpendicular to the slicing direction of the automatic pathological paraffin embedding wheel slicer; and a positioning block is provided at one end of the strip-shaped protrusion.

[0012] Preferably, a pair of strip protrusions of the positioning surface are symmetrically arranged at both ends of the positioning surface, and the distance between the two strip protrusions is not less than 1 / 3 of the length of the positioning surface; a pair of strip protrusions of the embedding sheet are symmetrically arranged at both ends of the embedding sheet and parallel to the wide side direction of the embedding sheet.

[0013] Preferably, the length direction of the strip-shaped protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip-shaped protrusion is not greater than 3 mm.

[0014] Preferably, the widths of the clamping adapter and the embedding sheet are not less than the width of the clamping adapter, the length of the embedding sheet is not less than the length of the clamping adapter, and the clamping surface of the clamping adapter is located in the width direction of the clamping adapter.

[0015] Preferably, the width of the clamping surface of the clamping adapter is 5mm-8mm, and the length of the positioning surface is 4cm and the width is 2.3cm.

[0016] Preferably, the embedding sheet has a length not exceeding 5 cm, a width not exceeding 4 cm, and a thickness not exceeding 2 mm; the protruding thickness of the large tissue section wax block on the embedding sheet is not greater than 4 mm; and hollow holes are evenly arranged on the embedding sheet.

[0017] Compared with the prior art, the present invention has achieved the following technical effects:

[0018] The present invention utilizes a clamping adapter to be fixedly connected to a sample clamp of a slicer; the embedding sheet and the clamping adapter are detachably assembled; a plurality of embedding sheets can be replaced for slicing by clamping the clamping adapter once, so that a large tissue slicing wax block can be quickly clamped and the position of the embedding sheet can be relatively fixed; therefore, the cutting angle can be maintained on the same cutting surface after repeated slicing, avoiding the phenomenon of repeatedly adjusting the cutting angle and over-shaving the lesion tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 Schematic diagram of the structure of the large tissue slice embedding clamping assembly in an embodiment of the present invention Figure 1 ;

[0021] Figure 2 Schematic diagram of the structure of the large tissue slice embedding clamping assembly in an embodiment of the present invention Figure 2 ;

[0022] Figure 3 Schematic diagram of the exploded structure of the large tissue slice embedding and clamping assembly according to an embodiment of the present invention;

[0023] Figure 4 Schematic diagram of the structure of the embedding sheet in the embodiment of the present invention Figure 1 ;

[0024] Figure 5 Schematic diagram of the structure of the embedding sheet in the embodiment of the present invention Figure 2 ;

[0025] Figure 6 Schematic diagram of the structure of the embedding sheet in the embodiment of the present invention Figure 3 ;

[0026] Figure 7 The structure diagram of the clamping adapter in the embodiment of the present invention is shown in FIG. Figure 1 ;

[0027] Figure 8 The structure diagram of the clamping adapter in the embodiment of the present invention is shown in FIG. Figure 2 ;

[0028] In the figure: 1-clamping adapter, 2-positioning surface, 3-oblique side surface, 4-step surface, 5-embedded sheet, 6-plug-in groove, 7-plug-in block, 8-hollow hole, 9-positioning stopper. DETAILED DESCRIPTION

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0030] It should be noted that, in the description of the present invention, the terms "upper", "lower", "left", "right", "inside", "outside", "front", "back", "clockwise", "counterclockwise" and the like indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and "fourth" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined as "first", "second", "third" and "fourth" may explicitly or implicitly include one or more of the features. In the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0031] Furthermore, it should be noted that, in the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0032] The purpose of the present invention is to provide a large tissue section embedding clamping assembly to solve the problems existing in the prior art, so that large tissue section wax blocks can be quickly clamped and can be kept on the same angle of section during repeated slicing.

[0033] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0034] Example 1

[0035] like Figures 1 to 8 As shown, this embodiment provides a large tissue section embedding clamping assembly, including a clamping adapter 2 and an embedding sheet 5. The clamping adapter 2 is provided with two parallel clamping surfaces on both sides. The clamping surfaces are used to clamp on the sample clamp of an automatic pathology paraffin embedding wheel microtome. The top of the two parallel clamping surfaces is connected by a positioning surface 2, and the end surface of the bottom is flush and can conflict with the bottom surface of the sample clamp. The positioning surface 2 is detachably connected to the embedding sheet 5, and the embedding sheet 5 is provided on the embedding sheet 5. In this embodiment, the clamping adapter 2 is fixedly connected to the sample clamp of the microtome. The embedding sheet 5 and the clamping adapter 2 are detachably assembled. The clamping adapter 2 is clamped once, and multiple embedding sheets 5 can be replaced for slicing. This allows for rapid clamping of large tissue section wax blocks and ensures that the position of the subsequent embedding sheet is relatively fixed. Therefore, the cutting angle can be maintained at the same cutting surface after repeated slicing, avoiding the phenomenon of repeatedly adjusting the cutting angle and cutting the lesion tissue too much.

[0036] As an optional solution, the clamping adapter 2 in this embodiment includes an oblique side surface 3, a stepped surface 4, a positioning surface 2, and two clamping surfaces. The positioning surface 2 is rectangular and is sequentially connected by the oblique side surface 3, a clamping surface, the stepped surface 4, and another clamping surface. The oblique side surface 3, the two clamping surfaces, the stepped surface 4, and the positioning surface 2 form a frame with a flat bottom and an open top. The two clamping surfaces are connected to the long sides of the positioning surface 2. The stepped surface 4 facilitates disassembly of the clamping adapter 2 by gripping the stepped surface 4 with one finger.

[0037] As an alternative, the material of the clamping adapter 2 in this embodiment includes metal or metal alloy, which is strong and durable, not easily deformed, ensures clamping accuracy, and is reusable. The embedding sheet 5 is made of polyester or polypropylene. Plastic materials are inexpensive and easy to process as disposable paraffin-bonding tools.

[0038] As an optional solution, in this embodiment, a plug-in slot 6 or a plug-in block 7 is provided on the positioning surface 2, and a plug-in block 7 or a plug-in slot 6 is provided on the back of the embedding sheet 5. The plug-in block 7 can be slidably inserted into the plug-in slot 6 to achieve a detachable connection, and the operation is simple, fast and convenient, which facilitates the rapid switching of different large tissue section wax blocks.

[0039] As an optional solution, in this embodiment, the plug-in slot 6 and the plug-in block 7 both include a pair of strip-shaped protrusions, the cross-section of the strip-shaped protrusion is L-shaped, T-shaped, a right-angled trapezoid or an isosceles trapezoid, and the cross-sectional size of the strip-shaped protrusion is preferably larger on the outside and smaller on the inside. There are both limiting surfaces parallel to the positioning surface 2 and limiting surfaces perpendicular to the positioning surface 2, which can prevent the embedded sheet 5 from moving during the slicing process, and the two pairs of strip-shaped protrusions can limit and clamp each other in the direction perpendicular to the strip-shaped protrusion and the positioning surface 2; a positioning stopper 9 is provided at one end of the strip-shaped protrusion, which facilitates the embedded sheet 5 to slide in from one end of the strip-shaped protrusion along the length direction of the strip-shaped protrusion and be positioned by the positioning stopper 9 at the other end to avoid excessive deviation of the position and limit the embedded sheet 5 at the same time; the length direction of the strip-shaped protrusion is perpendicular to the slicing direction of the automatic pathological paraffin embedding wheel slicer, and the strip-shaped protrusion is horizontal when in use.

[0040] As an optional solution, in this embodiment, a pair of protruding strips are symmetrically positioned at either end of the positioning surface 2, with the spacing between the two protruding strips being no less than 1 / 3 the length of the positioning surface 2. This ensures uniform force during slicing of the embedded slide 5. The pair of protruding strips are symmetrically positioned at either end of the embedded slide 5 and parallel to the broadside of the embedded slide 5, ensuring that they are perpendicular to the slicing direction of the automated paraffin-embedding wheel microtome.

[0041] As an optional solution, in this embodiment, the length direction of the strip-shaped protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip-shaped protrusion is no more than 3 mm, which occupies a small volume and can be staggered to save storage space.

[0042] As an optional solution, in this embodiment, the width of the clamping adapter 2 and the embedding sheet 5 is not less than the width of the clamping adapter 2, the length of the embedding sheet 5 is not less than the length of the clamping adapter 2, and the clamping surface of the clamping adapter 2 is located in the width direction of the clamping adapter.

[0043] As an optional solution, the width of the clamping surface of the clamping adapter 2 in this embodiment is 5mm-8mm, the length of the positioning surface 2 is 4cm, and the width is 2.3cm. The volume and shape are close to the existing small tissue section embedding wax block, and can be clamped directly without changing the sample clamp.

[0044] As an alternative, the embedding sheet 5 in this embodiment has a length of no more than 5 cm, a width of no more than 4 cm, and a thickness of no more than 2 mm. The thickness of the large tissue section wax block protruding from the embedding sheet 5 is no more than 4 mm, resulting in a total thickness of no more than 8 mm for the large tissue section embedding wax block, including the protruding strips. This overall thickness is significantly less than that of conventional embedding wax blocks. Hollow holes 8 are evenly distributed throughout the embedding sheet 5 to ensure sufficient connection surface and support for the paraffin embedding, preventing the wax block from cracking during sectioning.

[0045] Example 2

[0046] This embodiment provides a method for using a large tissue section embedding and clamping assembly, as follows:

[0047] First, the positioning surface 2 of the clamping adapter 2 is clamped upward and fixed on the sample fixture of the automatic pathology paraffin embedding wheel microtome. Then, the plug-in block 7 of the embedding sheet 5 carrying the large tissue section wax block is inserted into the plug-in slot 6 of the clamping adapter 2 from the side. The assembly and positioning process of the embedding sheet 5 only takes 2 seconds to complete, and the automatic pathology paraffin embedding wheel microtome can be turned on for sectioning. After completing the large tissue paraffin section, the embedding sheet 5 with the large tissue section wax block can be directly removed for archiving and storage. Since there is no clamping adapter 2 for storage, the storage volume is much smaller, which is convenient for long-term storage and reduces storage costs.

[0048] The entire operation process of this embodiment is simple and fast. There is no need to clamp the sample clamp multiple times and adjust the cutting angle, so that large tissue section wax blocks can be quickly clamped and the position of the rear embedding sheet 5 can be relatively fixed. Therefore, the cutting angle can be maintained at the same cutting plane after repeated slicing, avoiding the phenomenon of repeatedly adjusting the cutting angle and cutting the lesion tissue too much.

[0049] Throughout this specification, references to terms such as "one embodiment," "certain embodiments," "illustrative embodiments," "this embodiment," "specific examples," or "some examples" indicate that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, illustrative uses of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0050] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only intended to help understand the method and core concept of the present invention. At the same time, those skilled in the art will find that the specific implementation methods and application scopes may vary based on the concept of the present invention. In summary, the contents of this specification should not be construed as limiting the present invention.

Claims

1. A large tissue section embedding and clamping assembly, characterized by: It includes a clamping adapter and an embedding sheet. Two parallel clamping surfaces are provided on both sides of the clamping adapter. The clamping surfaces are used to clamp on the sample clamp of the automatic pathological paraffin embedding wheel slicer. The tops of the two parallel clamping surfaces are connected by a positioning surface, and the end surfaces of the bottoms are flush and can conflict with the bottom surface of the sample clamp. The embedding sheet is detachably connected to the positioning surface, and a large tissue section wax block is provided on the embedding sheet.

2. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The clamping adapter seat includes an oblique side surface, a step surface, the positioning surface and two clamping surfaces. The positioning surface is rectangular and is sequentially connected with the oblique side surface, one clamping surface, the step surface and another clamping surface on all four sides. The oblique side surface, the two clamping surfaces, the step surface and the positioning surface are spliced ​​to form a frame with a flat bottom and an open surface. The two clamping surfaces are connected to the long sides of the positioning surface.

3. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The material of the clamping adapter seat includes metal or metal alloy; the material of the embedding sheet includes polyester or polypropylene.

4. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The positioning surface is provided with a plug-in slot or a plug-in block, and the back side of the embedding sheet is provided with a plug-in block or a plug-in slot, and the plug-in block can be plugged into the plug-in slot.

5. The large tissue section embedding and clamping assembly according to claim 4, characterized in that: The plug-in slot and the plug-in block each include a pair of strip-shaped protrusions, the cross-section of the strip-shaped protrusions is L-shaped, T-shaped, a right-angled trapezoid or an isosceles trapezoid, and the two pairs of the strip-shaped protrusions can limit and clamp each other in a direction perpendicular to the strip-shaped protrusions and the positioning surface; the length direction of the strip-shaped protrusions is perpendicular to the slicing direction of the automatic pathological paraffin embedding wheel slicer; a positioning block is provided at one end of the strip-shaped protrusion.

6. The large tissue section embedding and clamping assembly according to claim 5, characterized in that: A pair of strip protrusions on the positioning surface are symmetrically arranged at both ends of the positioning surface, and the distance between the two strip protrusions is not less than 1 / 3 of the length of the positioning surface; a pair of strip protrusions on the embedding sheet are symmetrically arranged at both ends of the embedding sheet and parallel to the wide side direction of the embedding sheet.

7. The large tissue section embedding and clamping assembly according to claim 5, characterized in that: The length direction of the strip-shaped protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip-shaped protrusion is no more than 3 mm.

8. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The widths of the clamping adapter and the embedding sheet are not less than the width of the clamping adapter, the length of the embedding sheet is not less than the length of the clamping adapter, and the clamping surface of the clamping adapter is located in the width direction of the clamping adapter.

9. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The width of the clamping surface of the clamping adapter is 5mm-8mm, and the length of the positioning surface is 4cm and the width is 2.3cm.

10. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The embedding sheet has a length of no more than 5 cm, a width of no more than 4 cm, and a thickness of no more than 2 mm. The protruding thickness of the large tissue section wax block on the embedding sheet is no more than 4 mm. The embedding sheet is evenly provided with hollow holes.

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