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Method for simultaneously preparing yeast extract and beta-1,3-glucan

A technology of yeast extract and glucan, applied in food preparation, food science, application, etc., can solve the problem of low purity of 3-glucan products, insufficient yeast autolysis or enzymatic hydrolysis, yeast extract Low product yield and other problems, to shorten the preparation time, improve product purity, and achieve the effect of utilization

Inactive Publication Date: 2010-12-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies can realize the comprehensive utilization of beer waste yeast, but the preparation process only simply adopts acid method, alkali method or enzymatic method, etc., resulting in insufficient yeast autolysis or enzymatic hydrolysis, low yield of yeast extract products, and β -1,3-Glucan product is not high in purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Take 10kg of beer waste yeast mud (dry weight is 1.5kg), add 20kg of water, mix well and add 3gNaHCO 3 , after stirring for 1h, the gauze was filtered, and the filtrate was centrifuged at 3500r / min for 15min to obtain the sediment as standby yeast sludge, totally 3.3kg (dry weight is 0.61kg);

[0019] (2) Add 5 kg of water to the spare yeast sludge in step (1) and mix evenly, adjust the pH of the solution to 6, then add 1.3 g of papain, act at 60 ° C for 20 h, heat at 95 ° C for 15 min to inactivate the enzyme activity, 3800 r / Centrifuge for 15 min to obtain supernatant and precipitate;

[0020] (3) The supernatant of step (2) is dried with spray drying, the hot air inlet temperature is controlled at 170°C, and the outlet temperature is controlled at 80°C to obtain a total of 490g of powdery yeast extract, and the product yield is 80.3%. Amino nitrogen content is 3.58g / L;

[0021] (4) Add 2 times the NaOH solution of the weight of the precipitate to the precipit...

Embodiment 2

[0025] (1) Take 30kg of beer waste yeast mud (dry weight is 4.4kg), add 50kg of water, mix well and add 44gNaHCO 3 , after stirring for 0.5h, gauze was filtered, and the filtrate was centrifuged at 3500r / min for 15min to obtain the sediment as standby yeast slime, totally 10kg (dry weight was 1.86kg);

[0026] (2) Add 10kg of water to the spare yeast sludge in step (1) and mix evenly, adjust the pH of the solution to 5.5, then add 18g of papain, act at 50°C for 16h, and inactivate the enzyme (heating at 95°C for 15min), Centrifuge at 3800r / min for 15min to obtain supernatant and sediment;

[0027] (3) The supernatant of step (2) is dried by spray drying, the hot air inlet temperature is controlled at 170°C, and the outlet temperature is controlled at 80°C to obtain a total of 1597g of powdery yeast extract, with a yield of 85.9%. The nitrogen content is 3.73g / L;

[0028] (4) Add 4 times the NaOH solution of the weight of the precipitate to the precipitate obtained in step (2...

Embodiment 3

[0032](1) Take 20kg of beer waste yeast mud (dry weight is 2.8kg), add 20kg of water, mix well and add 14gNaHCO 3 After stirring for 0.5h, the gauze was filtered, and the filtrate was centrifuged at 3500r / min for 15min to obtain the sediment as standby yeast sludge, totally 7kg (dry weight is 1.3kg);

[0033] (2) Add 9 kg of water to the spare yeast sludge in step (1) and mix evenly, adjust the pH of the solution to 5.0, then add 13 g of papain, act at 45°C for 16h, heat at 95°C for 15min to inactivate the enzyme activity, 3800r / Centrifuge for 15 min to obtain supernatant and precipitate;

[0034] (3) The supernatant of step (2) is dried with spray drying, the hot air inlet temperature is controlled at 170°C, and the outlet temperature is controlled at 80°C to obtain a total of 1102g of powdery yeast extract, with a yield of 84.8%. The nitrogen content is 3.82g / L;

[0035] (4) Add 3 times the NaOH solution of the weight of the precipitate to the precipitate obtained in step...

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Abstract

The invention discloses a method for simultaneously preparing yeast extract and beta-1,3-glucan, which belongs to the field of preparation of yeast extract and beta-1,3-glucan. The method comprises the steps of bitter removal and impurity removal of beer waste yeast, enzymolysis and inactivation of the beer waste yeast, separation and drying of the yeast extract, acid and alkali treatment, washing and drying of the beta-1,3-glucan and the like. The method has the characteristics of high raw material utilization rate, good quality, low cost, easy industrialization and the like, realizes reutilization of the beer waste yeast, and fulfills the purpose of simultaneously producing two products comprising the yeast extract and the beta-1,3-glucan.

Description

technical field [0001] The invention relates to a method for producing yeast extract and β-1,3-glucan, in particular, to a method for simultaneously preparing yeast extract and β-1,3-glucan by using beer waste yeast Methods. Background technique [0002] Beer waste yeast is a by-product of beer production. It is a large amount of muddy yeast produced after the main fermentation and post-fermentation brewing process in the beer production process. It contains rich nutrients and functional components. [0003] Yeast extract is made from waste beer yeast. By degrading the protein in yeast cells into amino acids and polypeptides, and degrading nucleic acids into nucleotides, and extracting them and other active ingredients from yeast cells, the human body can A concentrate of soluble nutrients and flavor substances that can be directly absorbed and utilized. At present, there are three main methods for producing yeast extract: autolysis method, enzymatic hydrolysis method and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28C08B37/02A23L31/15
Inventor 薛文通武慧媛张泽俊于章龙
Owner CHINA AGRI UNIV
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