New way for synthesizing acetyl coenzyme A and derivative product thereof using glycolic aldehyde

An acetyl coenzyme and glycolaldehyde technology, applied in the field of biomedicine, can solve the problems of low reaction rate, low efficiency, and difficulty in accumulating high concentrations of glycolaldehyde, and achieve high catalytic activity, high reaction efficiency, and good stability.

Active Publication Date: 2017-05-31
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The reaction rate of the preparation method of above-mentioned acetyl phosphate is lower, and the conversion number K of phosphoketolase is for fructose cat Only 0.1/s, the efficiency is very low, and the long reaction time (18h) will test the stability of the enzyme and product
In addition, the third step, the cracking of D-erythrose into glycolaldehyde is a reversible process, and its reaction rate is affected by the concentration of glycolaldehyde after crac

Method used

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  • New way for synthesizing acetyl coenzyme A and derivative product thereof using glycolic aldehyde
  • New way for synthesizing acetyl coenzyme A and derivative product thereof using glycolic aldehyde
  • New way for synthesizing acetyl coenzyme A and derivative product thereof using glycolic aldehyde

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Experimental program
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Effect test

Embodiment 1

[0078] At an initial concentration of glycolaldehyde of 15 mM, the mutant enzyme Tal B of Transaldolase Tal B derived from Escherichia coli F178Y (On the basis of the original enzyme, the 178th amino acid residue is mutated from F to Y), add 5mM G3P, and carry out the preparation reaction of Ara5. After 1 hour of reaction, the reaction solution is lyophilized and derivatized, and quantitatively detected by GC-MS The obtained Ara5P content was 2.5mM, and the average synthesis rate of Ara5P was 52.1μmol(Ara5P) / min / mg(enzyme protein).

Embodiment 2

[0080] Glycolaldehyde at an initial concentration of 15mM, ThDP concentration was 1mM, PO 4 3+ The concentration was 2mM, and Fpk (EC 4.1.2.22) derived from Bifidobacterium adolescentis, aldolase (Fsa, EC 4.1.2.13) derived from Escherichia coli, 2mM G3P and trace amounts of Rpi A and Rpe were added to prepare AcP. After testing, the conversion rate of glycolaldehyde was 16.3 μmol (glycolaldehyde) / min / mg (enzyme protein).

[0081] The above data is the average rate of the reaction system for 3 hours.

Embodiment 3

[0083] Glycolaldehyde at an initial concentration of 15mM, ThDP concentration was 1mM, PO 4 3+ The concentration is 2mM, add the mutant enzyme Tal B of Xpk (EC 4.1.2.9) derived from Pseudomonas stutzeri A1501 and Transaldolase (Tal B, EC 2.2.1.2) derived from Escherichia coli F178Y , 2mM G3P and trace amounts of Rpi A and Rpe to prepare AcP. After testing, the conversion rate of glycolaldehyde was 19.7 μmol (glycol aldehyde) / min / mg (enzyme protein).

[0084] The above data is the average rate of the reaction system for 3 hours.

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Abstract

The invention discloses a new way for synthesizing acetyl coenzyme A and a derivative product thereof using glycolic aldehyde. The method comprises reaction that glycolic aldehyde reacts with 3-glyceraldehyde phosphate under enzyme catalysis to generate 5-phosphoric acid arabinose, wherein the enzyme is selected from aldolase, transaldolase and isozyme thereof and mutate enzyme. The method is relatively high in catalytic rate; the theoretical yield of carbon of a reaction route is 100%; the carbon loss avoided; G3P, an enzyme and a coenzyme all can be recycled; the reaction efficiency is relatively high; and the cost is reduced. In addition, the coenzymes RpiA and Rpe from a pentose phosphate pathway are high in affinity with a substrate and high in catalytic activity. According to the method, more obvious advantages are developed in the production processes, of in vitro continuous polyenzyme catalysis, fed-batch fermentation and continuous fermentation, capable of controlling the substrate level.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for synthesizing acetyl coenzyme A and derivatives thereof by using glycolaldehyde. Background technique [0002] Acetyl coenzyme A (AcCoA) is a key intermediate in the synthesis of essential biological compounds, including polyketides, fatty acids, isoprenoids, alkaloids, vitamins, and amino acids, among others. Metabolites derived from AcCoA are primary and secondary metabolites, which include compounds of industrial utility. AcCoA can be generated from acetyl phosphate (AcP) (for example, it can be catalyzed by phosphoacetyltransferase Pta (EC 2.3.1.8)), and then generate a series of biological products based on AcCoA platform, which are widely used in biocatalysis and other fields. The synthesis methods of AcP are: [0003] Patent application WO2015 / 144447A1 discloses a phosphoketolase (EC 4.1.2.9, fructose-6-phosphate phosphoketolase EC4.1.2.22) or sulfoacetal...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12P9/00C12P19/32C12P7/04C12P7/28C12P7/54C12P7/62C12P13/06C12P13/10C12P13/12C12P13/14C12P13/24
CPCC12P7/04C12P7/28C12P7/54C12P7/62C12P7/625C12P9/00C12P13/06C12P13/10C12P13/12C12P13/14C12P13/24C12P19/02C12P19/32
Inventor 马红武杨雪袁倩倩江会锋
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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