Method for synthesizing 2S,3R-p-methylsulfonephenylserine
A technology of thiamphenylphenylserine and p-thiamphenyl, applied in the field of synthesis of chiral 2S, 3R-p-thiamphenyl phenylserine, which can solve the problems of difficult reaction and difficult scale reaction
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[0021] Example 1. Preparation of chiral 2S, 3R-p-thiamphenicol phenylserine using transaldolase, aldehyde dehydrogenase and NAD oxidase
[0022] The reaction system (100 ml) in this example is composed of reaction solution, p-thymphenylbenzaldehyde, threonine, transaldolase, acetaldehyde condensing enzyme and NAD oxidase protein solution;
[0023] Among them, the reaction solution: 20 mM (pH 7.5) phosphate buffer solution; the concentration of p-thiamphenicyl benzaldehyde in the reaction system is 100 mM; transaldolase: the product number is GWTA08 commercial enzyme (purchased from Tianjin Industrial Microbiology Technology Co., Ltd. Company), the concentration in the reaction system is 1 mg / mL; acetaldehyde dehydrogenase, the product number is GWADR09 commercial enzyme (purchased from Tianjin Gong Microbiology Technology Co., Ltd.), the concentration in the reaction system is 0.5 mg / mL; NAD oxidase, the product number is GWAO16 commercial enzyme (purchased from Tianjin Gong M...
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