Process method for extracting ecdysone from cyanotis arachnoidea
By using needle-grade activated carbon and a multi-stage alcohol extraction and enzymatic hydrolysis process, the problem of low decolorization yield of activated carbon in the extraction of molting hormone from Dewdrop Grass was solved, achieving efficient extraction and improved purity, while reducing production difficulty and cost.
Patent Information
- Application Number
- CN202511409306.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2025-12-02
AI Technical Summary
Existing technologies for extracting ecdysone from dewgrass suffer from reduced yield and product quality fluctuations during activated carbon decolorization, making it difficult to effectively control the decolorization effect.
The decolorization process uses needle-grade activated carbon. By constructing specific adsorption sites on the surface of the activated carbon, combined with multi-stage alcohol extraction and enzymatic hydrolysis, the adsorption loss of ecdysone is reduced and the purity is improved.
This improved the extraction yield and purity of ecdysone, reduced production difficulty and cost, and ensured the stability of product quality.
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Figure CN121045305A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of plant active ingredient extraction technology, specifically to a process for extracting ecdysone from dewgrass. Background Technology
[0002] Dewdrop is a medicinal plant rich in ecdysone. Studies have shown that its β-ecdysone has potential medicinal value and is in significant demand in the agricultural and pharmaceutical fields. Ecdysone extracted from dewdrop (mainly β-ecdysone) has multiple potential medicinal uses, such as promoting protein synthesis, enhancing muscle strength, and relieving exercise fatigue, potentially for use in sports nutrition supplements or anti-fatigue drug development; by inhibiting the release of inflammatory factors, it has potential alleviating effects on chronic inflammation such as rheumatoid arthritis. Simultaneously, it also has neuroprotective effects and promotes wound healing, showing some protective potential against neurodegenerative diseases such as Alzheimer's disease, and can also be used in wound repair drugs or skin care products.
[0003] Currently, the extraction process of β-ecdysone from Dewdrop is the traditional water extraction or alcohol extraction process. However, regardless of the process route, the extract generally needs to undergo two decolorization steps: ion exchange resin decolorization and activated carbon decolorization, in order to improve the purity of the product.
[0004] This is mainly because ion exchange resins, during the initial decolorization process, can remove charged polar impurities from the extract, such as certain pigments (especially those containing ionic groups), organic acids, and alkaloids. Compared to activated carbon, ion exchange resins have weaker adsorption of target components like ecdysone, thus reducing the loss of active ingredients. Removing most polar impurities and pigments first using ion exchange resins reduces the adsorption load on activated carbon and improves the overall decolorization effect.
[0005] Activated carbon possesses broad-spectrum adsorption properties, capable of adsorbing non-polar or weakly polar pigments (such as fat-soluble pigments) and small-molecule organic impurities in the extract. It also effectively supplements impurities that are difficult to remove with ion exchange resins. After ion exchange resin pretreatment, activated carbon further adsorbs residual pigments and impurities, making the extract clearer and improving product purity. This two-step decolorization process, through complementary adsorption principles, targets impurities of different properties, achieving a process from coarse purification to deep purification, ultimately improving the purity and quality of ecdysone. However, due to the broad-spectrum adsorption properties of activated carbon (which also has high adsorption capacity for the target product ecdysone), adding too much activated carbon during the second-stage decolorization process can lead to a decrease in the overall product yield, while insufficient activated carbon can easily result in incomplete decolorization, causing a decline in product quality. Furthermore, the variations in raw materials from batch to batch make decolorization control difficult and result in significant quality fluctuations. Summary of the Invention
[0006] The purpose of this invention is to provide a process for extracting ecdysone from Dewdrop Grass that can improve the extraction yield and purity.
[0007] To achieve the above-mentioned objectives, the technical solution adopted by this invention is: a process for extracting ecdysone from dewwort, comprising the following steps:
[0008] S1. Pretreatment of medicinal materials: Crush the roots of the Dewdrop Grass;
[0009] S2. Alcohol extraction: Hot reflux extraction is performed using alcohol with a concentration of 50% or higher. After extraction, the extract is concentrated to a paste to obtain the extract.
[0010] S3. Water sedimentation: Add 3 times the amount of water to the extract, stir to dissolve and let stand for 1 hour, then filter through a 200-mesh sieve to discard the residue and obtain the filtrate;
[0011] S4. Resin adsorption and desorption: The filtrate is adsorbed through a macroporous adsorption resin column. After all the filtrate has been loaded, it is allowed to stand for 30 minutes. After rinsing with water until clear, the filtrate is desorbed using alcohol to obtain the desorbed solution.
[0012] S5. Decolorization: The eluent is concentrated and then decolorized once by passing it through an ion exchange resin column to obtain a primary decolorized solution; more than 10 times the volume of alcohol is added to dilute the primary decolorized solution to obtain a diluted solution; 0.2% of the total weight of the medicinal materials is added to the diluted solution with activated carbon for injection, the mixture is heated under reflux for 2 hours, and after standing and filtration, a secondary decolorized solution is obtained; the activated carbon for injection has adsorption and rejection properties against ecdysone;
[0013] S6. Post-treatment: The product is obtained by concentrating and drying the secondary decolorizing solution.
[0014] Preferably, in step S1, the roots of *Desmodium triflorum* are pulverized to 40-60 mesh, and deionized water is added at a solid-liquid ratio of 1:2-4, and stirred to form a uniform slurry; a compound enzyme is added at 0.5%-1% of the slurry mass, wherein the ratio of cellulase, hemicellulase and pectinase in the compound enzyme is 1:1:0.5; enzymatic hydrolysis is carried out at 40-55℃ for 1-2 hours; after enzymatic hydrolysis, the enzyme is heated to 100℃ for 10-15 minutes to inactivate it.
[0015] Preferably, in step S2, the roots of *Dewgrass* are subjected to reflux extraction three times; for the first extraction, 5-10 times the amount of alcohol is added and the mixture is refluxed for 2 hours; for the second extraction, 4-8 times the amount of alcohol is added and the mixture is refluxed for 2 hours; for the third extraction, 3-6 times the amount of alcohol is added and the mixture is refluxed for 2 hours; after the three extractions are completed, the extracts are combined.
[0016] Preferably, in steps S2, S4 and S5, the alcohol is methanol and the alcohol concentration is greater than 50%.
[0017] Preferably, the dew grass roots are fresh dew grass roots or dried dew grass roots;
[0018] When the roots of fresh water plants are dewy:
[0019] In step S1, deionized water is added at a solid-liquid ratio of 1:2, and compound enzyme is added at 0.5% of the slurry mass. Enzymatic hydrolysis is carried out for 1 hour.
[0020] In step S2, the amount of alcohol added for the three reflux extractions was 5 times, 4 times, and 3 times, respectively;
[0021] When the grass roots are dry and wet with dew:
[0022] In step S1, deionized water is added at a solid-liquid ratio of 1:4, and compound enzyme is added at 1% of the slurry mass. Enzymatic hydrolysis is carried out for 2 hours.
[0023] In step S2, the amount of alcohol added during the three reflux extractions was 10 times, 8 times, and 6 times, respectively.
[0024] Preferably, in step S4, during desorption, the solution is first rinsed with a 0.2% sodium hydroxide aqueous solution, then rinsed with clean water, and finally desorbed with an alcohol solution of more than four times the volume of the filtrate.
[0025] Preferably, the preparation method of the needle-grade activated carbon is as follows: 1.5 mmol of ecdysone, 6 mmol of functional monomer and 0.5 g of needle-grade activated carbon are added to 140 ml of acetonitrile and stirred until homogeneous; then 30 mmol of crosslinking agent and 0.2 g of initiator are added, and the mixture is ultrasonically treated for 5-10 min; then the mixture is heated in a 65°C water bath and stirred with a magnetic stirrer at a speed of 400-800 rpm for 24 h for polymerization reaction;
[0026] After the polymerization reaction was completed, the solid was filtered and the resulting solid was eluted with a 10% acetic acid methanol solution in a Soxhlet extractor to remove ecdysone molecules for 36-48 hours. The eluted activated carbon for injection was then vacuum dried at 55-65℃ for 12 hours to obtain ecdysone-imprinted activated carbon for injection.
[0027] Preferably, the crosslinking agent is trimethylolpropane trimethacrylate.
[0028] Preferably, the initiator is benzoyl peroxide.
[0029] Preferably, the activated carbon for needles is selected from activated carbon with a particle size of 0.10-0.15 mm.
[0030] The beneficial effects of this invention are mainly reflected in:
[0031] 1. By using needle-grade activated carbon with adsorption and rejection properties for ecdysone, the decolorization process avoids the loss of ecdysone and ensures the yield. At the same time, it does not hinder the adsorption of pigments with smaller molecules or other impurity molecules with structures that are very different from ecdysone, thus improving the purity of the product.
[0032] 2. The ecdysone of *Dewdrop* mainly exists within plant cells, and the plant cell wall, composed of cellulose, hemicellulose, and pectin, forms a physical barrier to solute dissolution. This invention employs enzymatic hydrolysis before alcohol extraction and effectively controls the enzymatic hydrolysis process parameters. This ensures the effective dissolution of the steroidal compound ecdysone while avoiding excessive enzymatic hydrolysis that leads to the dissolution of intracellular polysaccharides, proteins, pigments, and other impurities. This provides a guarantee for the efficient subsequent alcohol extraction.
[0033] 3. A multi-stage extraction strategy was adopted during the alcohol extraction process, which effectively ensured the content of ecdysone in the dew grass extract and improved the product yield.
[0034] 4. The overall process is easy to control, simple to operate, and conducive to standardized operations, which greatly reduces the difficulty of production and management, and has a positive impact on reducing the overall cost of the enterprise. Attached Figure Description
[0035] Figure 1 This is a flowchart of the process flow of the present invention. Detailed Implementation
[0036] The technical effects of the present invention will be described in detail below with reference to the embodiments.
[0037] Since the broad-spectrum adsorption performance of activated carbon mainly comes from the large number of internal pore spaces, these internal pore spaces are the "main force" for activated carbon to have a broad-spectrum adsorption capacity; in order to avoid the decrease in yield caused by the excessive adsorption of ecdysone due to the broad-spectrum adsorption characteristics of activated carbon during decolorization treatment.
[0038] This invention reverse-engineers molecular imprinting technology, constructing specific adsorption sites (imprinted layers) with weak binding adsorption capacity on the surface of activated carbon particles. When ecdysone approaches the activated carbon, it is first recognized and briefly bound by the imprinted pores on the surface (rather than directly entering the internal pores). This "surface occupancy" reduces the chance of it penetrating deep into the internal pores of the activated carbon (the main site of broad-spectrum adsorption). Simultaneously, the imprinted layer itself can partially cover or block the entrances of some non-specific pores, further reducing the probability of ecdysone entering these "strong adsorption regions." Pigment molecules are relatively small, and other impurity molecules have significant structural differences from ecdysone, so they are largely unaffected by the imprinted layer, allowing the activated carbon particles to maintain their broad-spectrum adsorption characteristics against pigments and other impurities. In other words, it can be simply viewed as "preferential surface recognition + reduced inward diffusion," allowing ecdysone to "stay at the entrance" rather than "go deep and be adsorbed," thereby reducing overall adsorption loss. The core idea is to avoid significant losses from strong binding adsorption through a limited amount of weak binding adsorption, while retaining its broad-spectrum adsorption capacity for pigments and other impurities.
[0039] Example 1
[0040] Preparation method of needle-grade activated carbon with adsorption and rejection properties for ecdysone:
[0041] 1.5 mmol of ecdysone, 6 mmol of functional monomer methacrylic acid, and 0.5 g of needle-grade activated carbon (purchased from Sichuan Ebian Huatai Activated Carbon Co., Ltd., 767 needle-grade activated carbon series, particle size 0.10-0.15 mm) were added to 140 ml of acetonitrile and stirred until homogeneous. Then, 30 mmol of crosslinking agent trimethylolpropane trimethacrylate and 0.2 g of initiator benzoyl peroxide were added, and the mixture was sonicated for 5-10 min. Afterward, the mixture was heated in a 65°C water bath and stirred with a magnetic stirrer at 400-800 rpm for 24 h for polymerization.
[0042] After the polymerization reaction was completed, the solid was filtered and the resulting solid was eluted with a 10% acetic acid methanol solution in a Soxhlet extractor to remove ecdysone molecules for 36-48 hours. The eluted activated carbon for injection was then vacuum dried at 55-65℃ for 12 hours to obtain ecdysone-imprinted activated carbon for injection.
[0043] Example 2
[0044] Same as Example 1, except that the particle size of the needle-grade activated carbon used is 0.20-0.25 mm.
[0045] Example 3
[0046] Same as Example 1, except that the particle size of the needle-grade activated carbon used is 0.05-0.09 mm.
[0047] Example 4
[0048] Take 3000g of fresh Dewdrop Grass roots, crush them to 40-60 mesh, add 6L of deionized water at a solid-liquid ratio of 1:2, stir to make a uniform slurry, add 45g of compound enzyme at 0.5% of the slurry mass, the compound enzyme contains 18g of cellulase, 18g of hemicellulase and 9g of pectinase; enzymatically hydrolyze at 40-55℃ for 1 hour; after enzymatic hydrolysis, heat to 100℃ for 10-15 minutes to inactivate.
[0049] Methanol was used for hot reflux extraction of the roots of *Hedyotis diffusa* in three stages, with a methanol concentration of 50% or higher during the reflux extraction. For the first extraction, five times the amount of methanol (50% or higher concentration) was added based on the total amount of 3000g of the herb, and the mixture was refluxed for 2 hours. To achieve a lossless conversion from the aqueous to the alcoholic system, based on the water content in the pre-enzymatic hydrolysis system, 9000g of 85% methanol was directly added for the first alcohol extraction, resulting in an actual methanol concentration of 51%. For the second extraction, four times the amount of methanol (12000g) with a concentration of 50% was added, and the mixture was refluxed for 2 hours. For the third extraction, three times the amount of methanol (9000g) with a concentration of 50% was added, and the mixture was refluxed for 2 hours. After all three extractions were completed, the extracts were combined and concentrated to a paste, yielding 82g of extract.
[0050] Add about 250g of water (3 times the volume of the extract), stir to dissolve and let stand for 1 hour, then filter through a 200-mesh sieve to discard the residue and obtain the filtrate;
[0051] The filtrate was adsorbed through a macroporous adsorption resin column. After all the filtrate was loaded, it was allowed to stand for 30 minutes. First, it was rinsed with 2000 ml of 0.2% sodium hydroxide aqueous solution at a flow rate of 50 mL / min, then rinsed with 15000 ml of water at a flow rate of 50 mL / min until clear. Finally, it was desorbed with 5000 ml of 80% methanol (the amount of methanol in this step should not be less than 4 times the volume of the filtrate to ensure complete desorption) to obtain the desorbed solution.
[0052] The desorption solution was concentrated and then decolorized once using an ion exchange resin column to obtain a primary decolorized solution. The solution was diluted with methanol at a volume greater than 10 times that of the primary decolorized solution to obtain a diluted solution. 6g of the activated carbon for injection prepared according to the method in Example 1 (0.2% of the total weight of the medicinal materials) was added to the diluted solution, and the mixture was heated under reflux for 2 hours. After standing and filtration, a secondary decolorized solution was obtained. The secondary decolorized solution was concentrated and dried to obtain 24.3g of *Hedyotis diffusa* extract, with an extract yield of 0.810%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 86.7%, with a final yield of 0.702%.
[0053] Example 5
[0054] Take 3000g of dried dew grass roots, crush them to 40-60 mesh, add 12L of deionized water at a solid-liquid ratio of 1:4, stir to make a uniform slurry, add 45g of compound enzyme at 1% of the slurry mass, the content of cellulase, hemicellulase and pectinase in the compound enzyme is 60g, 60g and 30g respectively; enzymatically hydrolyze at 40-55℃ for 2 hours; after enzymatic hydrolysis, heat to 100℃ for 10-15 minutes to inactivate.
[0055] Methanol was used for hot reflux extraction of the roots of *Hedyotis diffusa*, and the extraction was performed three times, with a methanol concentration of 50% or higher during the reflux extraction. For the first extraction, 10 times the amount of methanol (50% or higher concentration) was added based on the total amount of 3000g of the herb, and the mixture was refluxed for 2 hours. Based on the water content in the pre-enzymatic hydrolysis system, 18000g of 85% methanol was added for the first alcohol extraction, resulting in an actual methanol concentration of 51%. For the second extraction, 24000g of 50% methanol (8 times the amount of the herb) was added, and the mixture was refluxed for 2 hours. For the third extraction, 18000g of 50% methanol (6 times the amount of the herb) was added, and the mixture was refluxed for 2 hours. After all three extractions were completed, the extracts were combined and concentrated to a paste, yielding 351g of extract.
[0056] Add approximately 1050g of water (3 times the volume of the extract), stir to dissolve, and let stand for 1 hour. Then filter through a 200-mesh sieve to discard the residue and obtain the filtrate.
[0057] The filtrate was adsorbed through a macroporous adsorption resin column. After all the filtrate was loaded, it was allowed to stand for 30 minutes. First, it was rinsed with 2000 ml of 0.2% sodium hydroxide aqueous solution at a flow rate of 50 mL / min, then rinsed with 15000 ml of water at a flow rate of 50 mL / min until clear. Finally, it was desorbed with 6000 ml of 80% methanol (the amount of methanol in this step should not be less than 4 times the volume of the filtrate to ensure complete desorption) to obtain the desorbed solution.
[0058] The desorption solution was concentrated and then subjected to a first decolorization treatment using an ion exchange resin column to obtain a first decolorized solution. The solution was diluted with methanol at a volume greater than 10 times that of the first decolorized solution to obtain a diluted solution. 6g of the activated carbon for injection prepared according to the method in Example 1 (0.2% of the total weight of the medicinal materials) was added to the diluted solution, and the mixture was heated under reflux for 2 hours. After standing and filtration, a second decolorized solution was obtained. The second decolorized solution was concentrated and dried to obtain 122.5g of *Hedyotis diffusa* extract, with an extract yield of 4.083%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 84.2%, with a final yield of 3.438%.
[0059] Example 6
[0060] Similar to Example 4, except that the raw medicinal materials were pulverized but not enzymatically hydrolyzed. The final amount of *Dewdrop* extract was 17.8g, with an extract yield of 0.593%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 82.7%, with a final yield of 0.490%.
[0061] Example 7
[0062] Similar to Example 4, except that 1% of the slurry mass of the compound enzyme was added. The final amount of dewwort extract was 18.6g, with an extract yield of 0.620%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 78.9%, with a final yield of 0.489%.
[0063] Example 8
[0064] Similar to Example 4, except that 0.3% of the slurry mass of the compound enzyme was added. The final amount of dewwort extract was 18.2g, with an extract yield of 0.606%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 84.1%, with a final yield of 0.510%.
[0065] Example 9
[0066] Similar to Example 5, except that the enzymatic hydrolysis time was 3 hours. The final amount of dewwort extract was 131.8g, with an extract yield of 4.393%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 71.7%, with a final yield of 3.315%.
[0067] Example 10
[0068] Same as Example 5, except that the enzymatic hydrolysis time was 1 hour. The final amount of dewwort extract was 104.8g, with an extract yield of 3.493%. High-performance liquid chromatography (HPLC) analysis showed that the ecdysone content reached 82.4%, with a final yield of 2.878%.
[0069] As can be clearly seen from Examples 4 and 6-10, during the enzymatic hydrolysis process, excessive or insufficient amounts of compound enzyme, as well as excessively long or short hydrolysis times, have a significant impact on the final yield of ecdysone. This can lead to over- or under-hydrolysis. Therefore, for different medicinal materials (dried and fresh), it is necessary to control the amount of compound enzyme added and the hydrolysis time to achieve better hydrolysis results and improve the final yield.
[0070] To verify the adsorption rejection properties of the needle-grade activated carbon of this invention, ecdysone was extracted from *Desmodium styracifolium* according to the method described in Example 4. After treatment with an ion exchange resin column, the needle-grade activated carbon from Examples 1-3 (as experimental groups 1-3) and commercially available conventional activated carbon (as control groups 1-2) were used for secondary decolorization treatment. After the needle-grade activated carbon was saturated with adsorption, the adsorbate was desorbed, and the composition of the adsorbate was analyzed by high-performance liquid chromatography. The composition is shown in Table 1 below.
[0071] Control group 1: Purchased from Yunnan Qianxi Activated Carbon Co., Ltd., with a particle size of 0.1-1.15 mm;
[0072] Control group 2: Purchased from Yunnan Zhaotong Huanyi Activated Carbon Technology Co., Ltd., with a particle size of 0.1-1.15 mm;
[0073] Table 1. Adsorption Components of Activated Carbon for Needles
[0074] pigment impurities Ecstasy Example 1 42.8% 44.3% 12.9% Example 2 35.4% 37.4% 27.2% Example 3 27.4% 38.7% 33.9% Control group 1 24.5% 34.7% 40.8% Control group 2 29.7% 25.7% 44.6%
[0075] As can be seen from the table above, the needle-grade activated carbon described in this invention exhibits a lower adsorption capacity for ecdysone compared to ordinary activated carbon during the secondary decolorization adsorption process, effectively reducing the loss of the target component during decolorization adsorption. Furthermore, its adsorption rejection is significantly correlated with the particle size of the carbon source, but the specific reasons for this are currently unknown.
Claims
1. A process for extracting ecdysone from *Desmodium styracifolium*, characterized in that, Includes the following steps: S1. Pretreatment of medicinal materials: Crush the roots of the Dewdrop Grass; S2. Alcohol extraction: Hot reflux extraction is performed using alcohol with a concentration of 50% or higher. After extraction, the extract is concentrated into a paste to obtain the extract. S3. Water sedimentation: Add 3 times the amount of water to the extract, stir to dissolve and let stand for 1 hour, then filter through a 200-mesh sieve to discard the residue and obtain the filtrate; S4. Resin adsorption and desorption: The filtrate is adsorbed through a macroporous adsorption resin column. After all the filtrate has been loaded, it is allowed to stand for 30 minutes. After rinsing with water until clear, the filtrate is desorbed using alcohol to obtain the desorbed solution. S5. Decolorization: The eluent is concentrated and then decolorized once by passing it through an ion exchange resin column to obtain a primary decolorized solution; more than 10 times the volume of alcohol is added to dilute the primary decolorized solution to obtain a diluted solution; 0.2% of the total weight of the medicinal materials is added to the diluted solution with activated carbon for injection, the mixture is heated under reflux for 2 hours, and after standing and filtration, a secondary decolorized solution is obtained; the activated carbon for injection has adsorption and rejection properties against ecdysone; S6. Post-treatment: The product is obtained by concentrating and drying the secondary decolorizing solution.
2. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 1, characterized in that: In step S1, the roots of *Desmodium triflorum* are pulverized to 40-60 mesh, and deionized water is added at a solid-liquid ratio of 1:2-4. The mixture is stirred to form a uniform slurry. A compound enzyme is added at 0.5%-1% of the slurry mass, and the ratio of cellulase, hemicellulase, and pectinase in the compound enzyme is 1:1:0.
5. The enzyme is then enzymatically hydrolyzed at 40-55℃ for 1-2 hours. After the enzymatic hydrolysis is completed, the enzyme is heated to 100℃ for 10-15 minutes to inactivate it.
3. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 2, characterized in that: In step S2, the roots of Dewdrop Grass are subjected to reflux extraction three times. For the first extraction, 5-10 times the amount of alcohol is added and the mixture is refluxed for 2 hours. For the second extraction, 4-8 times the amount of alcohol is added and the mixture is refluxed for 2 hours. For the third extraction, 3-6 times the amount of alcohol is added and the mixture is refluxed for 2 hours. After the three extractions are completed, the extracts are combined.
4. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 3, characterized in that: In steps S2, S4 and S5, the alcohol is methanol with a concentration greater than 50%.
5. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 4, characterized in that: The dew grass roots are either fresh or dried. When the roots of fresh water plants are dewy: In step S1, deionized water is added at a solid-liquid ratio of 1:2, and compound enzyme is added at 0.5% of the slurry mass. Enzymatic hydrolysis is carried out for 1 hour. In step S2, the amount of alcohol added during the three reflux extractions was 5 times, 4 times, and 3 times, respectively; When the grass roots are dry and wet with dew: In step S1, deionized water is added at a solid-liquid ratio of 1:4, and compound enzyme is added at 1% of the slurry mass. Enzymatic hydrolysis is carried out for 2 hours. In step S2, the amount of alcohol added during the three reflux extractions was 10 times, 8 times, and 6 times, respectively.
6. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 5, characterized in that: In step S4, during desorption, the solution is first rinsed with a 0.2% sodium hydroxide aqueous solution, then rinsed with clean water, and finally desorbed with an alcohol solution of more than four times the volume of the filtrate.
7. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 6, characterized in that: The preparation method of the needle-grade activated carbon is as follows: 1.5 mmol of ecdysone, 6 mmol of functional monomer and 0.5 g of needle-grade activated carbon are added to 140 ml of acetonitrile and stirred until homogeneous; then 30 mmol of crosslinking agent and 0.2 g of initiator are added and ultrasonically treated for 5-10 min; then the mixture is heated in a 65°C water bath and stirred with a magnetic stirrer at a speed of 400-800 rpm for 24 h for polymerization reaction; After the polymerization reaction was completed, the solid was filtered and the resulting solid was eluted with a 10% acetic acid methanol solution in a Soxhlet extractor to remove ecdysone molecules for 36-48 hours. The eluted activated carbon for injection was then vacuum dried at 55-65℃ for 12 hours to obtain ecdysone-imprinted activated carbon for injection.
8. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 7, characterized in that: The crosslinking agent is trimethylolpropane trimethacrylate.
9. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 8, characterized in that: The initiator is benzoyl peroxide.
10. The process for extracting ecdysone from *Hymenochloa crus-galli* according to claim 9, characterized in that: The activated carbon used for needles is selected from activated carbon with a particle size of 0.10-0.15 mm.