Proton-based transcriptome sequencing data comparison and analysis method and system

A technology for transcriptome sequencing and sequencing data, which is applied in the field of biological information to achieve the effect of improving accuracy and reliability
CN104657628AInactive Publication Date: 2015-05-27BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2015-05-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a Proton-based transcriptome sequencing data comparison and analysis method and system. The method comprises the following steps: acquiring original sequencing data of at least two transcriptomes of a certain species by virtue of a Proton sequencing platform; filtering unqualified data to obtain clean reads; performing first-step analysis and second-step analysis, wherein the first-step analysis comprises the steps of comparing the clean reads with a reference genome of the species respectively, performing transcript quantitative analysis, screening significantly differently expressed genes and performing significantly differently expressed gene function analysis; the second-step analysis comprises the steps of comparing the clean reads to the reference genome of the species respectively, performing alternative splicing analysis, performing gene structure optimization analysis, performing new transcript prediction, performing SNP (Single Nucleotide Polymorphism) analysis and performing gene fusion detection. According to the method and the system, the transcriptome sequencing data comparison and analysis accuracy and reliability can be improved.
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Description

technical field

[0001] The present invention relates to the technical field of biological information, in particular to a method and system for comparative analysis of transcriptome sequencing data based on Proton. Background technique

[0002] Transcriptome broadly refers to the collection of all transcripts in a cell under certain physiological conditions, including messenger RNA (mRNA), ribosomal RNA, transfer RNA and non-coding RNA; in a narrow sense, it refers to the collection of all messenger RNA. Since the transcriptome represents the gene expression state of an organism at a certain moment, the study of the transcriptome has great biological significance.

[0003] A gene can have multiple differently spliced ​​transcripts that produce mRNA, and mRNA can also be produced from other regions on the genome. Sequencing technology is a powerful tool for investigating and understanding mRNA expression profiles. Compared with the traditional chip hybridization platform, t...

Claims

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