The invention relates to a method which uses
microsatellite marking technology for identifying the
animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the
microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of
genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects
microsatellite marks with specific property among the types and universal property in the type, designs
specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of
rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow
disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.