37 results about "Mad Cow Diseases" patented technology

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.

The present invention relates to the device for animal inspection and quarantine and the preparation method thereof. The present invention is especially applicable to the detection of the pathogen of bovine spongiform encephalopathy (BSE) (also known as “mad cow disease”). The present invention employs a piezoelectric chip, a microelectrode array and a common electrode fixed on the lower side surface and upper side surface of the piezoelectric chip, respectively, and a BSE PrP antibody array to constitute the piezoelectric biochip for the detection of the BSE pathogen. The BSE PrP antibodies are immobilized on the electrodes of the microelectrode array in a format corresponding uniquely to the electrodes of the microelectrode array by adsorbing, bonding, cross-linking, embedding or self-assembly process. The combination of the piezoelectric biochip and a detector constitutes the piezoelectric biochip detection system for the BSE pathogen. When the antibodies react with the corresponding PrPs immunochemically, the information about the PrPs can be detected at real time by measuring the resonant frequency, and the PrPs thus can be analyzed qualitatively and quantitatively. The present invention is applicable to the early, effective and rapid detection of the BSE pathogen.

The disclosed invention relates to sterilization by infrared radiation to eliminate pathogenic bacteria such as Salmonella, E. Coli 0157:H7 and E. Coli (EXEC) from articles such as medical, dental and veterinary instruments, as well as from tableware and eating utensils. The invention further relates to sterilization of soil, military and agriculture equipment to eliminate pathogenic bacteria such as hepatitis, AIDS, and anthrax, and prions such as mad cow disease using infrared radiation. Sterilization is performed by exposing the article to infrared radiation generated from heating elements positioned in an enclosed chamber.

Disclosed herein are compounds and methods for decreasing PrP and preventing, ameliorating, or treating a prion disease or conformational neurodegenerative disorder, in an individual in need thereof. Examples of disease conditions that can be ameliorated with the administration of antisense compounds targeted to PrP include Creutzfeldt-Jakob disease (CJD); variant Creutzfeldt-Jakob Disease (vCJD); Gerstmann-Straussler-Scheinker syndrome; fatal familial insomnia; kuru; Bovine Spongiform Encephalopathy (BSE), e.g. “mad cow disease”; Chronic Wasting Disease (CWD); scrapie; transmissible mink encephalopathy; feline spongiform encephalopathy; ungulate spongiform encephalopathy; Alzheimer's disease; Parkinson's disease; Huntington's disease; and Amyotrophic Lateral Sclerosis (ALS).

The present invention relates to a manufacturing technique of fetal bovine serum. The present invention includes the full closed aseptic collection, low temperature separation and microporous membrane filtration, which is characterized in that: the present invention carries out the caesarean section of a cow which is approximately eight months pregnant, lateral decubitus restraint method is adopted before the delivery, and the anesthesia adopts the method with the combination of general anesthesia and local anesthesia at the surgical site. The full closed aseptic cardiac puncture for blood sampling is carried out on the fetal bovine; after the collection of the blood, a large-capacity refrigerated centrifuge is used for centrifugation of 30 minutes as the rotation speed of 3400 turns per minute, and the separated serum is collected in a sterile container under the local class 100 area for rapid cryopreservation. The present invention has the advantage that: the price of the imported fetal bovine serum is higher, which reaches about 6,000 RMB per liter. The imported fetal bovine serum from the United States and Europe not only has high cost, but also has the hidden dangers of mad cow disease. The price of the fetal bovine serum which is produced by our manufacturing technique is just 4000 to 5000 RMB per liter, which completely changes the status that the fetal bovine serum in China entirely dependents on the foreign imports.

The invention provides cloned transgenic ungulates (e.g., bovines) in which prion protein activity is reduced by one or more genetically engineered mutations. Desirably, these transgenic bovines are also genetically modified to express xenogenous (e.g., human) antibodies. Because of their resistance to prion-related diseases such as bovine spongiform encephalopy (also known as mad cow disease), these bovines are a safer source of human antibodies for pharmaceutical uses and safer source of agricultural products.

Methods for diagnosing altered neuropathology in an animal are disclosed, wherein said methods comprise imaging brain, spinal cord, or other neural tissue of the animal, analyzing the appearance of the tissue, and determining whether the appearance of the tissue is altered relative to corresponding unaltered tissue. Also disclosed are methods for diagnosing spongiform encephalopathies in an animal, wherein said methods comprise imaging brain, spinal cord, or other neural tissues of the animal, analyzing the appearance of vacuoles in the tissue, and determining whether the appearance of the vacuoles in the tissue is altered relative to corresponding spongiform encephalopathy-free tissue. Also disclosed are automated methods for diagnosing altered neuropathy and spongiform encephalopathies.

The invention discloses a preparation method of a collagen peptide-zinc chelate. According to the preparation method, fresh shellfish is used as raw materials; modified shellfish pallium water-solublecollagen is obtained by a hot extraction method; then, the water-soluble collagen is hydrolyzed by protease to obtain collagen peptide; the collagen peptide is chelated with metallic zinc ions; and the collagen peptide-zinc chelate is obtained. The method provided by the invention has the advantages that processing wastes of marine organisms are used as the raw materials, the acquisition path issimple and convenient; the cost is low; the chelation rate is high; and risks such as mad cow diseases and foot-and-mouth diseases possibly occurring in terrestrial animal collagen are avoided. Meanwhile, compared with zinc ions, the peptide-zinc chelate obtained after the collagen peptide is chelated with the metallic zinc ions has the advantages that the toxic and side effects are small; the synergistic effect of the collagen peptide and the zinc ions can be achieved; and safe and good-effect anti-osteoporosis functional food can be provided for customers.

The invention relates to a novel application of ginsenoside Rd in preparing a drug for treating microglial cell mediated diseases, and particularly relates to a novel application of ginsenoside Rd in preparing a drug for treating diseases such as disseminated sclerosis, amyotrophic lateral sclerosis, HIV-associated dementia, mad cow disease and the like.

The invention discloses a culture medium for preparing tetanus toxin and a method for preparing the tetanus toxin from the culture medium. The culture medium contains an organic nitrogen source of a yeast source, the mass percent of the organic nitrogen source of an animal source and/or a plant source in the organic nitrogen source of the culture medium is smaller than 1-50%. According to the culture medium, the risk of introducing zoonotic diseases such as mad cow disease is avoided, strain anabiosis and seed culture can be met, and produced toxin is also far higher than the requirements of the existing standard. The operation of preparing the tetanus toxin by utilizing the culture medium is simple, the cost is low, and the method is favorable for industrial production.

The invention belongs to the field of biological materials and particularly relates to a fishskin collagen aggregates based bone conductive material and a preparation method and application thereof. The material can be widely applied to the field of bone tissue repair and plastic surgery, and the bone conductive material is obtained by taking natural fishskin collagen aggregates with moderate treatment as carriers and plant polyphenols and aldehyde groups as media and solidifying fish bones subjected to pelletizing processing on the surface and internal pores of the fishskin collagen aggregates. The material can be directly prepared by taking the fishskin collagen aggregates as the carriers, better natural bionic three-dimensional structure and better mechanical properties are achieved, and potential risk of human and zoonotic diseases such as mad cow disease and foot and mouth diseased caused by the use of animal source materials such as pigs and cows is avoided.

The invention provides a nicergoline lyophilized preparation with excellent stability. The nicergoline lyophilized preparation is prepared by freeze-drying a nicergoline drug solution, and each 1000 gof the nicergoline drug solution comprises: 2-4 g of nicergoline, 0.5-1 g of tartaric acid, 0.2-9 g of sodium chloride, 20-40 g of an excipient, and an appropriate amount of water for injection, andthe pH value of the drug solution is 3.0-5.0. In the formula of the lyophilized preparation, mannitol is used to replacing lactose in an original development agent to be a lyophilized excipient, which can avoid the risks of allergies, pyrogens, and mad cow disease caused by lactose as an injection adjuvant, and overcomes the instability caused by the absence of lactose in the prior art. The mostnotable feature of the preparation is the combination of mannitol, sorbitol and the like with sodium chloride, and the nicergoline for injection still has excellent stability without using of nicergoline. The lyophilized product of the invention has rapid reconstitution and loose appearance, and is convenient for transportation and preservation and clinical use.

Methods for diagnosing altered neuropathology in an animal are disclosed, wherein said methods comprise imaging brain, spinal cord, or other neural tissue of the animal, analyzing the appearance of the tissue, and determining whether the appearance of the tissue is altered relative to corresponding unaltered tissue. Also disclosed are methods for diagnosing spongiform encephalopathies in an animal, wherein said methods comprise imaging brain, spinal cord, or other neural tissues of the animal, analyzing the appearance of vacuoles in the tissue, and determining whether the appearance of the vacuoles in the tissue is altered relative to corresponding spongiform encephalopathy-free tissue. Also disclosed are automated methods for diagnosing altered neuropathy and spongiform encephalopathies.

The invention discloses a kind of nucleotide sequence for detecting the special risk of mad cow disease of beef and its method as well as reagent. The group of nucleotide sequence has a base sequence shown in the sequence from SEQ ID No.1 to SEQ ID No.3. The advantage of the invention is that: it judges whether there is GFAP mRNA in the beef or not to determine whether the beef is contaminated by the risk of mad cow disease. Comparing with the traditional ELISA technology, the method is more sensitive, rapid, and better stability.

The invention relates to a method for preparing a fish scale gelatin plasma substitute injection. The method for preparing the fish scale gelatin plasma substitute injection includes the steps that fish scale gelatin serves as a raw material, and the plasma substitute injection is obtained through the production processes of gelatin degradation, cross-linking modification, electrolyte adding, adsorption, filtering, filling, sterilization, lamp inspection, packaging and the like. The method has the advantages that the plasma substitute injection is prepared from the fish scale gelatin, the low cost of the raw material is guaranteed, potential safety risks, caused when a gelatin raw material from animals such as pigs and cows possibly carries epidemic diseases such as a foot-and-mouth disease and a mad cow disease, of the human body are avoided, and a fish scale gelatin plasma substitute has higher biosecurity; succinic anhydride is used as a cross-linking agent, and therefore the toxic and side effects on the human body are avoided; a proper quantity of electrolytes are added, and therefore it is guaranteed that electrolyte disturbance can not be caused when a large quantity of fish scale gelatin plasma substitutes are injected; as a porous titanium bar and a microporous filtering film are combined to carry out classified filtering, the filtering speed is greatly increased, and the method is better suitable for large-scale production.