Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fetal cattle blood serum production technology

A technology of fetal bovine serum and production process, applied in the field of fetal bovine serum, can solve the hidden danger of mad cow disease and the high cost of fetal bovine serum, and achieve the effects of improving product level, technical level and high cost

Inactive Publication Date: 2008-01-30
兰州民科生物技术中心
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Importing fetal bovine serum from the United States and Europe is not only expensive, but also has the hidden danger of mad cow disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] The key technologies for the collection of fetal bovine serum are mainly caesarean section of pregnant cows and fully enclosed aseptic heart puncture blood collection of fetal cattle. Caesarean section is performed on the cows who are pregnant for about 8 months. Before delivery, the method of lying on the side is used, and the two front legs are tied and pulled forward, and the two hind legs are tied and pulled backward. The head is fixed with a rope. During anesthesia, general anesthesia and local anesthesia at the surgical site are combined, that is, general anesthesia is anesthetized by 2% procaine by lumbar nerve trunk conduction, and local anesthesia is used to anesthetize the 18th intercostal nerve, iliohypogastric nerve and ilioinguinal nerve respectively. . Effective anesthesia is an important guarantee for a smooth caesarean section. Therefore, identify the injection site, insert the needle vertically to the anterior or posterior corner of the free end of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a manufacturing technique of fetal bovine serum. The present invention includes the full closed aseptic collection, low temperature separation and microporous membrane filtration, which is characterized in that: the present invention carries out the caesarean section of a cow which is approximately eight months pregnant, lateral decubitus restraint method is adopted before the delivery, and the anesthesia adopts the method with the combination of general anesthesia and local anesthesia at the surgical site. The full closed aseptic cardiac puncture for blood sampling is carried out on the fetal bovine; after the collection of the blood, a large-capacity refrigerated centrifuge is used for centrifugation of 30 minutes as the rotation speed of 3400 turns per minute, and the separated serum is collected in a sterile container under the local class 100 area for rapid cryopreservation. The present invention has the advantage that: the price of the imported fetal bovine serum is higher, which reaches about 6,000 RMB per liter. The imported fetal bovine serum from the United States and Europe not only has high cost, but also has the hidden dangers of mad cow disease. The price of the fetal bovine serum which is produced by our manufacturing technique is just 4000 to 5000 RMB per liter, which completely changes the status that the fetal bovine serum in China entirely dependents on the foreign imports.

Description

technical field [0001] The invention relates to a fetal bovine serum, more specifically to a production process of the fetal bovine serum. Background technique [0002] Fetal bovine serum is the blood of healthy pregnant cows around eight months old, which is collected aseptically, separated at low temperature, and subpackaged by microporous filtration. It has an excellent cell growth-promoting effect, and is suitable for the preservation of primary and passage cell lines and the cultivation of delicate cells, and is mainly used in high-level, precise, and advanced biological and medical experiments. At present, due to domestic technical reasons, there is no real fetal bovine serum for the time being, and almost all rely on imports. The price of imported fetal bovine serum is relatively high, reaching about 6,000 yuan per liter. Importing fetal bovine serum from the United States and Europe is not only expensive, but also has the hidden danger of mad cow disease. Contents...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D1/08A61D1/00B01D17/00B01D35/00B01D57/00A61K35/16
Inventor 马忠仁富小刚
Owner 兰州民科生物技术中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com