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Transgenic ungulates having reduced prion protein activity and uses thereof

A prion protein, prion technology, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problem of lack of knockout vector and/or nuclear transfer method, etc.

Active Publication Date: 2006-03-01
KYOWA HAKKO KIRIN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To our knowledge, the successful production of transgenic cattle with mutations at the prion locus has not been reported, possibly due to the lack of suitable knockout vectors and / or nuclear transfer methods

Method used

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  • Transgenic ungulates having reduced prion protein activity and uses thereof
  • Transgenic ungulates having reduced prion protein activity and uses thereof
  • Transgenic ungulates having reduced prion protein activity and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0190] Example 1: Transgenic ungulates with reduced prion protein activity

[0191] Bovine fibroblast clones in which both alleles of the PrP locus were mutated were produced by sequential homologous recombination. will be used to produce PrP homozygous DNA constructs for knockout cells were used to prevent transcription of functional full-length PrP mRNA. Consequently, the resulting immature PrP transcript lacks a functional coding region. First, a DNA construct containing the neo gene (ie, the PrPKOneo vector) was electroporated into a bovine fibroblast cell line, and neomycin-resistant clones were then isolated. According to PCR analysis, homologous recombination occurred in exon 3 in some clones. Thus, bovine fibroblasts were generated in which one allele in the PrP locus was mutated. From hemizygous mutant (hem-PrPKO) fibroblasts, fetuses in which one allele of the three PrP loci were mutated were generated and hemizygous fibroblast lines were re-established. Then, a...

Embodiment 2

[0212] Embodiment 2: Import and rearrangement of HAC

[0213] Summary of methods used to insert HACs

[0214] For the production of ungulate alleles (such as cattle) that have mutations in one or both alleles of the prion gene and express heterologous antibodies, conventional methods can be used to insert heterologous antibody-expressing nucleic acids (such as HAC) into in cells. For example, the HAC can be inserted before or after one or both alleles of the prion gene are inactivated. One or more endogenous antibody genes in the cell are also mutated, if desired. The cells are then used in conventional nuclear transfer procedures to produce the desired transgenic ungulates, as described in more detail below.

[0215] In fact, male and female bovine fetal fibroblast cell lines containing human artificial chromosomes (eg, #14fg., #2fg., and #22fg.) were obtained and cloned calves were produced from these cell lines.

[0216] For example, simultaneous or sequential insertion...

Embodiment 3

[0313] Example 3: Xenoantibody-producing transgenic ungulates with mutations in one or more endogenous antibodies

[0314] In ungulates expressing xenogeneic antibodies and having mutations in prion genes as described in Example 2, endogenous antibody levels can optionally be reduced by mutating one or more endogenous antibody genes. Express. The number of B cells expressing a desired xenogeneic antibody (e.g., a human therapeutic antibody) by increasing the number of functional xenogeneic immunoglobulin heavy or light chain genes relative to the number of functional endogenous heavy and light chain genes will increase.

[0315] To produce these transgenic ungulates, ΔHAC or ΔΔHAC transgenic ungulates can be made to contain mutations in one or more alleles of endogenous immunoglobulin chains (such as μ heavy chains or lambda or kappa light chains) mating of transgenic ungulates. If desired, the resulting transgenic ungulate can be associated with (i) one or both alleles of ...

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PUM

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Abstract

The invention provides cloned transgenic ungulates (e.g., bovines) in which prion protein activity is reduced by one or more genetically engineered mutations. Desirably, these transgenic bovines are also genetically modified to express xenogenous (e.g., human) antibodies. Because of their resistance to prion-related diseases such as bovine spongiform encephalopy (also known as mad cow disease), these bovines are a safer source of human antibodies for pharmaceutical uses and safer source of agricultural products.

Description

technical background [0001] In general, the invention features cloned transgenic ungulates (eg, cattle) in which the activity of prion protein (PrP) is reduced by one or more genetically engineered mutations. Since such transgenic cattle with reduced prion activity are resistant to prion-related diseases such as bovine spongiform encephalopathy (BSE, also known as mad cow disease), they are safer and more preferred candidates for therapeutic antibodies in agricultural products and pharmaceuticals such as humans. origin of. [0002] Since the first case of BSE was detected in the UK in 1986, the contagious disease has spread to other parts of the world, such as Japan. The impact of this disease has greatly affected the agricultural and pharmaceutical industries, limiting the use of cattle products in these industries. [0003] Based on more than a decade of research, prions have been identified as the true cause of this infectious disease. Animals with reduced prion protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00A01K67/027C12N15/00C12N5/02C12N5/06C12N5/16C12N5/10
Inventor J·罗布尔Y·库罗伊瓦P·科拉斯E·叙利文P·卡西纳坦K·托米祖卡I·伊施达
Owner KYOWA HAKKO KIRIN CO LTD
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