Method for preparing fish scale gelatin plasma substitute injection

A fish scale gelatin and injection technology, which is applied in blood diseases, pharmaceutical formulations, extracellular fluid diseases, etc., can solve the problem of high cost of raw materials, and achieve the effects of high cross-linking degree, accurate control of reaction conditions, and improved filtration speed.

Inactive Publication Date: 2016-04-06
WUHAN HAIJIYA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the raw materials for the preparation of gelatin-substituted plasma mainly come from the skin and bones of pigs, cattle and other animals. Because they may carry epidemic diseases such...

Method used

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  • Method for preparing fish scale gelatin plasma substitute injection

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] 1. Degradation of fish scale gelatin: Dissolve 210g of fish scale gelatin (32g based on nitrogen content) with 1500ml of water for injection heated to 90°C, adjust the pH value of the fish scale gelatin solution to 5.5 with hydrochloric acid or sodium hydroxide solution, and dissolve the fish scale gelatin Heat the gelatin solution to 115°C, keep warm and monitor the relative viscosity of the fish scale gelatin solution, when the relative viscosity reaches 1.7, stop heating immediately, and cool to 40°C to obtain a fish scale gelatin degradation solution;

[0035] 2. Cross-linking modification: adjust the fish scale gelatin degradation solution with hydrochloric acid or sodium hydroxide solution to make the pH value to 8.5, keep the temperature at 40°C, add 1.6g of succinic anhydride, stir continuously, and cross-link for 2 hours;

[0036] 3. Add electrolyte: add 2500ml of water for injection to the fish scale gelatin cross-linking solution, add 1.1g of potassium chlorid...

example 2

[0041] 1. Degradation of fish scale gelatin: Dissolve 220g of fish scale gelatin (32g in terms of nitrogen content) in 2000ml of water for injection heated to 80°C, adjust the pH value of the fish scale gelatin solution to 6 with hydrochloric acid or sodium hydroxide solution, and dissolve the fish scale gelatin Heat the gelatin solution to 120°C, keep warm and monitor the relative viscosity of the fish scale gelatin solution, when the relative viscosity reaches 1.8, stop heating immediately, and cool to 35°C to obtain a fish scale gelatin degradation solution;

[0042] 2. Cross-linking modification: adjust the fish scale gelatin degradation solution with hydrochloric acid or sodium hydroxide solution to make the pH value to 9, keep the temperature at 35°C, add 2.52g of succinic anhydride, stir continuously, and cross-link for 2 hours;

[0043] 3. Add electrolyte: Add 2500ml of water for injection to the fish scale gelatin cross-linking solution, add 1.1g of potassium chloride,...

example 3

[0048] 1. Degradation of fish scale gelatin: Dissolve 205g of fish scale gelatin (32g in terms of nitrogen content) in 2500ml of water for injection heated to 70°C, adjust the pH value of the fish scale gelatin solution to 6.5 with hydrochloric acid or sodium hydroxide solution, and dissolve the fish scale gelatin Heat the gelatin solution to 125°C, keep warm and monitor the relative viscosity of the fish scale gelatin solution, when the relative viscosity reaches 1.9, stop heating immediately, and cool to 30°C to obtain a fish scale gelatin degradation solution;

[0049] 2. Cross-linking modification: adjust the fish scale gelatin degradation solution with hydrochloric acid or sodium hydroxide solution to make the pH value to 9.5, keep the temperature at 30°C, add 1.95g of succinic anhydride, stir continuously, and cross-link for 2 hours;

[0050] 3. Add electrolyte: Add 2000ml of water for injection to the fish scale gelatin cross-linking solution, add 1.1g of potassium chlor...

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Abstract

The invention relates to a method for preparing a fish scale gelatin plasma substitute injection. The method for preparing the fish scale gelatin plasma substitute injection includes the steps that fish scale gelatin serves as a raw material, and the plasma substitute injection is obtained through the production processes of gelatin degradation, cross-linking modification, electrolyte adding, adsorption, filtering, filling, sterilization, lamp inspection, packaging and the like. The method has the advantages that the plasma substitute injection is prepared from the fish scale gelatin, the low cost of the raw material is guaranteed, potential safety risks, caused when a gelatin raw material from animals such as pigs and cows possibly carries epidemic diseases such as a foot-and-mouth disease and a mad cow disease, of the human body are avoided, and a fish scale gelatin plasma substitute has higher biosecurity; succinic anhydride is used as a cross-linking agent, and therefore the toxic and side effects on the human body are avoided; a proper quantity of electrolytes are added, and therefore it is guaranteed that electrolyte disturbance can not be caused when a large quantity of fish scale gelatin plasma substitutes are injected; as a porous titanium bar and a microporous filtering film are combined to carry out classified filtering, the filtering speed is greatly increased, and the method is better suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for preparing fish scale gelatin instead of plasma injection using fish scale gelatin as a raw material. Background technique [0002] The annual medical blood consumption in our country is as high as 1300 tons. In recent years, the blood consumption is still increasing at a rate of 7% to 10% per year. The rapid increase in clinical blood consumption has caused the shortage of blood to reach a crisis level. Clinically, 90% of hepatitis C is transmitted by blood transfusion. Among the key infectious diseases, the case of transfusion infection has been ranked first for many years, and accidents of human immunodeficiency virus infection due to blood transfusion occur frequently. Blood shortage, difficulty in storage and transportation, high price and many other reasons make blood transfusion more and more cautious in clinic, and reduce the chance of blood transfusion as much...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K9/08A61P7/08
Inventor 周湘汉刘萍董恩钊
Owner WUHAN HAIJIYA BIOTECH CO LTD
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