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Preparation method of collagen peptide-zinc chelate

A collagen peptide and zinc chelate technology, applied in the field of biochemistry, can solve the problems of low zinc yield and unsatisfactory chelation effect, and achieve low cost, easy access and good effect

Inactive Publication Date: 2019-12-20
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chelating effect of the collagen peptide obtained from the common type I collagen as raw material is not satisfactory when chelating zinc, and the yield of collagen peptide chelating zinc is low

Method used

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  • Preparation method of collagen peptide-zinc chelate
  • Preparation method of collagen peptide-zinc chelate
  • Preparation method of collagen peptide-zinc chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Select 100 g of fresh pearl oyster mantle, wash and remove impurities with distilled water, and cut it into pieces with a size of 2 cm×2 cm at a temperature of 2 to 4° C. to obtain raw materials. Add 0.1mol / L NaOH solution to the prepared pearl oyster mantle, the mass ratio of raw material to NaOH solution is 1:10, stir for 24 hours in an environment with a temperature of 4°C, and then centrifuge at a speed of 10000rpm , remove the supernatant, then repeatedly add distilled water to wash the raw material 5 times, so that the raw material is neutral, soak the pretreated pearl oyster mantle with distilled water, the mass ratio of raw material to distilled water is 1:10, and the temperature is 90 ° C. Water bath extraction is carried out in the environment, and finally the insoluble matter is removed by suction filtration, and the filtrate is freeze-dried to obtain hot water to extract collagen. Dissolve the prepared collagen in distilled water at 10 mg / mL, adjust the pH t...

Embodiment 2

[0041] Embodiment 2 (comparative example):

[0042] Dissolve 500 mg of commercially available tilapia collagen peptide in 5 mL of distilled water to make a collagen peptide solution, adjust the pH to 6, dissolve zinc sulfate in distilled water to form a solution with a concentration of 100 mg / mL, and mix the zinc sulfate solution with collagen The protein-peptide solution was mixed at a volume ratio of 1:5.5, stirred and dissolved, and chelated at a temperature of 45°C for 45 minutes, and then absolute ethanol was added to the mixed solution so that the final concentration of ethanol in the solution reached 80%. Stand still at ℃ for 45 minutes, centrifuge at 10,000 rpm for 10 minutes to take the precipitate, then wash the precipitate repeatedly with absolute ethanol, and centrifuge 5 times. The precipitate was collected and dried at a temperature of 50° C. to obtain a peptide-zinc chelate, and finally 0.093 g of the chelate was obtained.

Embodiment 3

[0044] Select 100 g of fresh pearl oyster mantle, wash and remove impurities with distilled water, and cut it into pieces with a size of 2 cm×2 cm at a temperature of 2 to 4° C. to obtain raw materials. Add 0.08mol / L NaOH solution to the prepared pearl oyster mantle, the mass ratio of raw material to NaOH solution is 1:8, stir for 48h in an environment with a temperature of 2°C, and then centrifuge at a speed of 12000rpm , remove the supernatant, then repeatedly add distilled water to wash the raw material 7 times, so that the raw material is neutral, soak the pretreated pearl oyster mantle with distilled water, the mass ratio of raw material to distilled water is 1:8, and the temperature is 100 ℃. The water-bath extraction is carried out in the environment, and finally the insoluble matter is removed by suction filtration, and the collagen is obtained after freeze-drying. Dissolve the prepared collagen in distilled water at 15 mg / mL, adjust the pH to 7.0, add pepsin with 2% c...

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Abstract

The invention discloses a preparation method of a collagen peptide-zinc chelate. According to the preparation method, fresh shellfish is used as raw materials; modified shellfish pallium water-solublecollagen is obtained by a hot extraction method; then, the water-soluble collagen is hydrolyzed by protease to obtain collagen peptide; the collagen peptide is chelated with metallic zinc ions; and the collagen peptide-zinc chelate is obtained. The method provided by the invention has the advantages that processing wastes of marine organisms are used as the raw materials, the acquisition path issimple and convenient; the cost is low; the chelation rate is high; and risks such as mad cow diseases and foot-and-mouth diseases possibly occurring in terrestrial animal collagen are avoided. Meanwhile, compared with zinc ions, the peptide-zinc chelate obtained after the collagen peptide is chelated with the metallic zinc ions has the advantages that the toxic and side effects are small; the synergistic effect of the collagen peptide and the zinc ions can be achieved; and safe and good-effect anti-osteoporosis functional food can be provided for customers.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a preparation method of a collagen peptide-zinc chelate. Background technique [0002] Bone homeostasis is regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. Factors such as environment, age, and genetics can lead to the imbalance of bone homeostasis and the degradation of bone microstructure, which in turn can lead to diseases such as osteoporosis and osteoarthritis. At present, the treatment of osteoporosis mostly focuses on the use of estradiol, calcitonin, bisphosphonates and other methods. Long-term use of these drugs will cause a lot of side effects. Therefore, it is of strategic significance to find and lead the mainstream functional food in the 21st century to find long-term edible, safe and effective food and base materials based on the theory of homology of medicine and food. [0003] Collagen is widely distributed in human and...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/107C12P21/06
CPCC07K14/78C12P21/06
Inventor 申铉日
Owner HAINAN UNIVERSITY
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