Method for identifying source constituent in meat product by micro-satellite labeling technique

Abstract

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.

Description

1. Technical field [0001] The invention relates to a method for identifying ingredients derived from meat products using microsatellite marker technology, which belongs to the high-tech field of biology. It is of great significance in the inspection of animal feed, food and medicine. 2. Background technology [0002] Although my country's meat product market management has introduced many policies and measures, most of them are related to the detection of meat quality, water, starch and other things, and there are few identifications on the type of meat products. However, there are many counterfeit mutton in the market Or beef and other phenomena. For example, some people mix sheep fat or butter with dead pork, put it in plastic bags and freeze it, and then it becomes "mutton roll" and "beef roll". In particular, the discovery and prevalence of diseases such as mad cow disease and sheep scrapie in the last century made the ingredients of bovine and sheep origin in food, medi...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of difficult identification of animal-derived ingredients in animal feed, food, and drug inspection, and to provide a method for identifying ingredients derived from cattle, sheep, pigs, dogs, chickens, and ducks in meat products using microsatellite marker technology. method

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