Full-automatic gel-preparation electrophoresis ultraviolet glue sweeping and cutting instrument

A glue cutting machine, fully automatic technology, applied in the field of experimental instruments, can solve the problems of damage to the recovery strip, long duration, human body harm, etc., and achieve the effect of reasonable structure design, ensuring accuracy, and avoiding pollution

Inactive Publication Date: 2011-05-04
蔡鎏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current traditional method, these four steps are completed manually. In this process, due to the manual contact with high-risk drugs such as ethidium bromide, the experimenters will wear gloves during the experiment, but due to the multi-step process of the experiment The experimenter must put on and take off the gloves many times, and always pay attention to avoid the gloves from polluting other instruments in the laboratory. However, since the glue is exposed to the air during the experiment, due to the volatility of ethidium bromide itself, it will be very To a certain extent, it causes air pollution in the laboratory, especially for the experimente...

Method used

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  • Full-automatic gel-preparation electrophoresis ultraviolet glue sweeping and cutting instrument
  • Full-automatic gel-preparation electrophoresis ultraviolet glue sweeping and cutting instrument
  • Full-automatic gel-preparation electrophoresis ultraviolet glue sweeping and cutting instrument

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1. When using a fully automatic gel-making electrophoresis UV-sweeping glue-cutting instrument of the present invention, only the three-layer device on the left is used. First, the program is input on the numerical control panel 10022, and the setting of the numerical control integrated automatic glue-making equipment 109 is set. Parameters, CNC automatic weighing instrument 1091 weighs out the required agarose powder, and CNC automatic liquid adder 1092 adds the required buffer solution to the glass test tube (not shown in the figure) inside it. After adding the liquid, the manipulator After clamping the small tray with a groove on one side of the weighed agarose powder, pour the agarose powder into glass test tubes, put them into the microwave heater 1093 for heating via the manipulator, and shake the test tube for several times during the heating process by the manipulator. The second time, the manipulator 105 adjusts the movable baffle according to the req...

Embodiment 2

[0046] Embodiment 2, when a fully automatic gel-making electrophoresis UV-sweeping gel-cutting instrument of the present invention is in use, only the three-layer device on the right is used for the processing of ordinary identification gels. This process does not need to cut the gel. Input the program on 10022, set the parameters of the CNC integrated automatic glue-making equipment 109, the CNC automatic weighing instrument 1091 weighs out the required agarose powder, and the CNC automatic liquid adder 1092 adds the required buffer solution to its internal In the glass test tube, after adding the liquid, the manipulator clamps the weighed agarose powder into the small container, then pours the agarose powder into the glass test tube, and puts the agarose powder into the microwave heater 1093 for heating via the manipulator 105. Shake the test tube several times during the heating process. The manipulator 105 adjusts the movable baffle according to the requirements of the expe...

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PUM

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Abstract

The invention discloses a full-automatic glue-preparation electrophoresis ultraviolet glue sweeping and cutting instrument which comprises a box body. The box body is divided into three layers, a baffle is arranged in the box body, the baffle divides the interior of the box body into six cells on the left side and the right side, the upper part of the box body is provided with a panel, and a liquid crystal screen, a numerical control panel and a control circuit respectively connected with the liquid crystal screen and the numerical control panel are arranged on the panel of the box body respectively; the left side and the right side of the second layer of the box body are respectively provided with a periscope view port; and in the six cells, an elevator, a drying device, an automatic cleaning device, an air-exhaust liquid-exhaust device, a mechanical arm, a plastic gel supporting plate, an adjustable glue preparation tank, a stirring rod, microcontrol integrated automatic glue preparation equipment, a miniature sample adding gun, an ethidium bromide container, a liquid adding device, a periscope, an electrophoresis tank, an ultraviolet glue sweeping camera, a glue cutting instrument, a centrifuge tube clamp and an ethidium bromide dye vat are comprised. The full-automatic glue-preparation electrophoresis ultraviolet glue sweeping and cutting instrument disclosed in the invention has the advantages of automation, integration, convenience and quickness, and greatly increases the efficiency, the precision and the safety of experiments.

Description

technical field [0001] The invention relates to an experimental instrument, in particular to a fully automatic gel electrophoresis ultraviolet sweeping gel cutting instrument. Background technique [0002] In molecular biology experiments, in order to identify and extract target gene fragments, preparation of agarose gel, electrophoresis, ultraviolet scanning and photographing, and gel cutting and recovery are all indispensable steps. In the current traditional method, these four steps are completed manually. In this process, due to the manual contact with high-risk drugs such as ethidium bromide, the experimenters will wear gloves during the experiment, but due to the multi-step process of the experiment The experimenter must put on and take off the gloves many times, and always pay attention to avoid the gloves from polluting other instruments in the laboratory. However, since the glue is exposed to the air during the experiment, due to the volatility of ethidium bromide i...

Claims

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Application Information

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IPC IPC(8): C12M1/00G01N27/447
Inventor 蔡鎏
Owner 蔡鎏
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