SNP marker developing and application of rice broad-spectrum rice blast resistance gene Pigm

Abstract

The invention provides an SNP molecular marker K_060508 which is tightly linked to a rice broad-spectrum rice blast resistance gene Pigm. It is detected by the SNP marker that the basic group at the 10421726 site of the 6 chromosome of rice is G or A, and the primer combination of the SNP marker developed based on a KASP technology is Primer X, Primer Y and Primer C. According to the SNP marker, the KASP technology is utilized to perform rapid genotyping on the SNP marker which is tightly linked to the rice blast resistance gene Pigm, the SNP marker can be applied to commercial molecular seedling breeding with high, medium or low through-put, the selectivity efficiency of a developed SNP marker phenotype reaches up to 100%, and therefore the broad-spectrum rice blast resistance gene Pigm can be rapidly and accurately detected in different germ plasm resources of indica rice, japonica rice and the like; the complicated procedures of enzyme digestion, electrophoresis, sequencingand the like are not needed in the detecting process, aerosol contamination is reduced, the use of toxic substances of EB (ethidium bromide) and the like is reduced, outlook molecular marker auxiliaryselection at the early stage of seedling breeding can be conducted to reduce the field planting scale of seedling breeding groups and the seedling breeding cost, and the seedling breeding process isaccelerated.

Description

technical field [0001] The invention relates to the fields of molecular markers and crop breeding, in particular to the development and application of SNP markers of rice broad-spectrum rice blast resistance gene Pigm. Background technique [0002] Rice blast caused by Magnapotheoryzae is a worldwide rice planting disease. The annual loss of rice yield due to rice blast can reach 11% to 30%. Long-term practice shows that cultivating disease-resistant varieties (lines) It is the most economical and effective measure to control rice blast at present. In recent decades, with the development of molecular genetics, molecular marker-assisted selection has played a pivotal role in rice disease resistance breeding. The use of efficient molecular marker-assisted selection of single plants containing target genes for hybridization can accurately perform target traits. It can reduce the scale of field planting of selected breeding groups, shorten the breeding period, and save breeding...

AI Technical Summary

Problems solved by technology

However, most of the molecular markers used to detect Pigm are SSR, InDel or CAPS markers. These markers cannot effectively distinguish Pigm from other multiple alleles, and cannot completely distinguish Pigm donors and acceptors, and the detection results are likely to cause misjudgment.

In addition, the detection process of SSR, InDel or CAPS and other markers requires enzyme digestion, gel electrophoresis and other steps. The operation is complicated, the experimental cost is high, the detection throughput is small, and it is easy to pollute the environment and cause harm to the human body.

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