Pigeon sex discriminating method

Abstract

The invention belongs to the field of molecular biology and discloses a pigeon sex discriminating method comprising the following steps of: designing an upstream primer SEQIDNO.1 (Sequence Identification Number 1) and a downstream primer SEQIDNO.2 by comparing a 2550F / 2718R primer amplified product of a pigeon CHD gene with a CHD gene sequence landed on NCBI (National Center for Biotechnology Information); amplifying CHD gene fragments (preparing a genomic DNA template from micro feather pulp cells) on Z and W sex chromosomes of a pigeon by PCR (Polymerase Chain Reaction); dyeing by EB (ethidium bromide); judging sex according to a band type through 1 percent of agarose gel electrophoresis; appearing two bands, namely that 628bp and 358bp are male; and appearing three bands, namely that 628bp, 420bp and 358bp are female. The invention combines a new preparation method of an animal cell genomic DNA template and a high-efficiency pigeon CHD gene fragment amplimer, and the once detection rate and the detection accuracy of the pigeon sex discrimination can reach 100 percent.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a pigeon sex identification method. Background technique [0002] Accurate early pairing of pigeons is one of the practical techniques in the pigeon farm. Pigeons are strictly monogamous. If there is a mismatch, not only will the pigeons fight, but the hens will not lay eggs. At present, except for a few breeds of pigeons that can distinguish between male and female based on their feathers, most breeds of pigeons, including squabs, young pigeons, and even adult pigeons, cannot be identified through morphological methods such as body shape, body posture, and anus turning. Although very experienced breeders can identify individual adult males and females by body shape, posture, etc., the accuracy rate is not high. [0003] Bird sex chromosomes are composed of ZZ (male) and ZW (female), and thus developed in recent years to identify the sex of birds based on the length of the amplifi...

AI Technical Summary

Problems solved by technology

The disadvantage of the above methods is that taking animal blood samples or picking multiple main wing feathers is easy to cause harm to birds or pigeons; most of the genomic DNA extraction methods use conventional methods, which are not only cumbersome in the extraction process, but also cannot ensure that the genomic DNA is extracted once. and the cost is relatively high; most of the primers for PCR identification use the above two general primers and other corresponding primers, but the one-time detection rate is actually not ideal

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