Pigeon sex discriminating method

A sex and template technology, applied in the field of molecular biology, can solve the problems of not being able to ensure the one-time success of genomic DNA extraction, the one-time detection rate is not ideal, and the extraction process is cumbersome, so as to ensure the one-time success of the preparation and reduce the extraction cost. The effect of low cost and simple preparation process

Inactive Publication Date: 2011-01-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the above methods is that taking animal blood samples or picking multiple main wing feathers is easy to cause harm to birds or pigeons; most of the genomic DNA extraction methods use conventional methods, which are not only cumbersome in the extraction process, but also cannot ensure that the genomic DNA is extracted once. and the cost is relatively high; most of the primers for PCR identification use the above two general primers and other corresponding primers, but the one-time detection rate is actually not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pigeon sex discriminating method
  • Pigeon sex discriminating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Squab sex identification

[0015] 1.1 Preparation of genomic DNA template of squab feather marrow cells

[0016] Take one ventral feather (containing a small amount of feather marrow) from a newborn squab about 7 days old, and put about 1 mg of feather marrow into a PCR tube;

[0017] Add 8.8 μl of trace animal cell genomic DNA template preparation solution to the PCR tube containing feather marrow;

[0018] Put the PCR tube containing the feather marrow and a small amount of animal cell genomic DNA template preparation solution in a PCR machine for 5 minutes at 95°C, 5 minutes at 16°C, 2 hours at 55°C, 10 minutes at 95°C, and 5 minutes at 16°C. And during the first 5 minutes at 16°C, the PCR instrument was suspended, and 1.2 μl of proteinase K solution with a concentration of 10 mg / ml was added. The centrifuged supernatant was directly used as a DNA template for PCR;

[0019] Wherein, the above-mentioned trace preparation liquid of animal cell genome DNA t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of molecular biology and discloses a pigeon sex discriminating method comprising the following steps of: designing an upstream primer SEQIDNO.1 (Sequence Identification Number 1) and a downstream primer SEQIDNO.2 by comparing a 2550F / 2718R primer amplified product of a pigeon CHD gene with a CHD gene sequence landed on NCBI (National Center for Biotechnology Information); amplifying CHD gene fragments (preparing a genomic DNA template from micro feather pulp cells) on Z and W sex chromosomes of a pigeon by PCR (Polymerase Chain Reaction); dyeing by EB (ethidium bromide); judging sex according to a band type through 1 percent of agarose gel electrophoresis; appearing two bands, namely that 628bp and 358bp are male; and appearing three bands, namely that 628bp, 420bp and 358bp are female. The invention combines a new preparation method of an animal cell genomic DNA template and a high-efficiency pigeon CHD gene fragment amplimer, and the once detection rate and the detection accuracy of the pigeon sex discrimination can reach 100 percent.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a pigeon sex identification method. Background technique [0002] Accurate early pairing of pigeons is one of the practical techniques in the pigeon farm. Pigeons are strictly monogamous. If there is a mismatch, not only will the pigeons fight, but the hens will not lay eggs. At present, except for a few breeds of pigeons that can distinguish between male and female based on their feathers, most breeds of pigeons, including squabs, young pigeons, and even adult pigeons, cannot be identified through morphological methods such as body shape, body posture, and anus turning. Although very experienced breeders can identify individual adult males and females by body shape, posture, etc., the accuracy rate is not high. [0003] Bird sex chromosomes are composed of ZZ (male) and ZW (female), and thus developed in recent years to identify the sex of birds based on the length of the amplifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 邢光东夏银茆骏李银
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap