Method for effectively and quickly detecting high reproductive trait of sheep through BMP15 gene

Abstract

The utility model provides a method for effectively and quickly detecting high reproductive trait of sheep through BMP15 gene. The method comprises the following steps: 1) a SNP locus is newly found in a BMP15 gene coded sequence of Qira black sheep and positioned in a Genebank sequence, and a T-C mutation (T755C for short) occurs at a +755 locus by taking the initiator codon ATG of the BMP 15 gene sequence as the beginning; 2) a pair of nucleotide sequences are synthesized to serve as primers for detecting T755 C locus, the forward primer is 5'-gaagaccaaacctctccctaagg-3', the reverse primer is 5'-tactttcaggcccatcatgctcc-3', and both upstream and downstream primers are 23bp in length, the primers are combined on the corresponding template strands of the sheep BMP 15 gene, and amplified to the nucleotide sequence including an E+755 locus; 3) DNA is extracted, PCR is amplified through the primers, and the product is detected by 2% agarose gel electrophoresis and dyed by ethidium bromide, so that the amplified target fragment can be determined by gel imaging; and 4) the PCR product is digested by restriction enzyme Apal, and when the enzyme digestion product is fragments 120 bp and 140 bp, an individual is proven to have a high reproductive performance.

Description

technical field [0001] The invention relates to a single nucleotide polymorphism (SNP for short) site on the BMP15 gene coding sequence of Cele black sheep, which can be used as a molecular marker for high reproductive traits of sheep, and establishes a detection method for determining the genotype of the SNP site method. Background technique [0002] Sheep are single-born mammals, but some sheep breeds have stable multiple births. According to the study, the BMPR-IB gene, GDF9 gene and BMP15 gene are of great value in controlling the multifetal performance of sheep. In particular, it was found that the base changes in the above three gene sequences can significantly affect the reproductive traits of sheep. The FecB gene is the first multifetal main gene found in sheep. This gene is produced by a mutation of a base in the BMPR-IB gene. It is also found in GDF9 and BMP15 that can affect the lambing performance of sheep. [0003] In multiparity sheep in my country, the FecB ...

AI Technical Summary

Problems solved by technology

[0003] FecB gene has been found to significantly affect litter size in multiparity sheep in my country. This gene has been widely used as a molecular marker in production practice; however, the research on other multiparity main genes has not yet reached a clear conclusion

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