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Method for detecting ethidium bromide by fluorescent carbon dot probe

A technology of ethidium bromide and fluorescent carbon dots, applied in the field of chemical detection, to achieve low cytotoxicity, sensitive detection, and good biocompatibility

Inactive Publication Date: 2015-04-29
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there are no relevant reports on the use of fluorescent carbon dot fluorescent probes for the detection of ethidium bromide.

Method used

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  • Method for detecting ethidium bromide by fluorescent carbon dot probe
  • Method for detecting ethidium bromide by fluorescent carbon dot probe

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Experimental program
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Embodiment 1

[0021] Prepare four standard solutions, in which the amount of fluorescent carbon dots is 20 μL, add ethidium bromide solution to the fluorescent carbon dot solution, so that the final concentration of ethidium bromide is 0, 1×10 -6 mol / L, 3×10 -5 mol / L and 6×10 -5 mol / L, let stand for 1min, and detect the fluorescence spectrum at 380-650nm at an excitation wavelength of 375nm, and get the fluorescence spectrum diagrams a, b, c and d in sequence.

[0022] It can be seen from the fluorescence spectrum that the fluorescence intensity of the fluorescent carbon dots decreases with the increase of the concentration of ethidium bromide solution, and the reduction value of fluorescence intensity has a good linear relationship with the concentration of ethidium bromide solution, R 2 = 0.999.

[0023] The present invention uses fluorescent carbon dots as probes for highly sensitive detection of ethidium bromide. There is a good linear relationship between the fluorescence intensity c...

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Abstract

The invention discloses a method for detecting ethidium bromide by a fluorescent carbon dot probe. The method is characterized in that a fluorescent carbon dot is used as the probe, so that high-sensitivity detection is carried out based on the characteristic that fluorescence intensity of the fluorescent carbon dot is weakened when the concentration of ethidium bromide solution is increased, the change value of the fluorescence intensity of the fluorescent carbon dot and the ethidium bromide concentration form a good linear relationship, and the relevant coefficient is 0.999. The method is simple in operation, fast in detection, high in sensitivity and excellent in selectivity, thus rapid online in-situ sensitive detection can be executed to ethidium bromide in a mixed sample by the method.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a method for detecting ethidium bromide by using a fluorescent carbon dot probe. Background technique [0002] Ethidium bromide is a highly sensitive fluorescent stain used to visualize DNA in agarose and polyacrylamide gels. But ethidium bromide is a strong carcinogen, which can be embedded in base molecules, causing mismatches. Harmful in contact with skin and if swallowed, extremely toxic if inhaled, irritating to eyes, respiratory system and skin, with possible danger of irreversible consequences. [0003] Therefore, the analysis and detection of ethidium bromide is particularly important. So far, the detection methods of ethidium bromide include high performance liquid chromatography, electrochemical method and physical and chemical detection of pharmaceutical environment. However, these methods have disadvantages such as cumbersome pretreatment pro...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 黄珊肖琦王鲁敏谢江宁
Owner GUANGXI TEACHERS EDUCATION UNIV
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