Rapid detection technology of high-sensitivity pathogenic nucleic acid for non-diagnosis and treatment purposes

Abstract

The invention relates to the technical field of virus detection. The invention provides a rapid detection technology of high-sensitivity pathogenic nucleic acid for non-diagnosis and treatment purposes. The rapid detection technology comprises the following steps of (1) carrying out isothermal amplification on recombinase; and (2) carrying out color developing by colloidal gold. According to the rapid detection technology for the high-sensitivity pathogenic nucleic acid, isothermal nucleic acid amplification-colloidal gold color development is used for pathogen field detection, special equipment and reagent are not needed, meanwhile, the pathogen extraction step is omitted, pathogen nucleic acid signals can be captured and amplified to the maximum extent, sputum, blood, excrement and othersamples of a patient can be directly detected, and the technology is very suitable for field detection, bedside diagnosis of hospitals, crowd gathering and living scenes such as schools and airportsand application under the condition of few other resources, and is particularly suitable for scenes where results need to be obtained rapidly. The defects that in the prior art, pathogen nucleic aciddetection depends on large equipment and reagent, the detection period is long, the sensitivity is low, and pathogen needs to be extracted can be overcome.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a rapid detection technology for highly sensitive pathogenic nucleic acid for non-diagnostic and therapeutic purposes. Background technique [0002] The most important basis of molecular diagnosis is nucleic acid amplification technology. The current mainstream DNA amplification technology requires sophisticated temperature-changing equipment, complex kits (with high requirements for storage and transportation), complex sample processing procedures and professionals, so its application in on-site applications and crowd detection is very limited. [0003] At present, nucleic acid detection of new coronavirus pneumonia mostly adopts reverse transcription PCR fluorescence method, which takes a long detection cycle and takes at least 48-72 hours. It relies on large, special, expensive equipment, reagents and professionals, and the sensitivity of detection is about 30% to 50%....

AI Technical Summary

Problems solved by technology

The current mainstream DNA amplification technology requires sophisticated temperature-changing equipment, complex kits (with high requirements for storage and transportation), complex sample processing procedures and professionals, so the application in field applications and crowd detection scenarios is very limited.

[0003] At present, nucleic acid detection of new coronavirus pneumonia mostly adopts reverse transcription PCR fluorescence method, which takes a long detection cycle and takes at least 48-72 hours. It relies on large, special, expensive equipment, reagents and professionals, and the sensitivity of detection is about 30% to 50%.

At the same time, virus nucleic acid (RNA) extraction and enrichment must be carried out after the samples of the new coronavirus pneumonia virus are collected, and the existing virus collection methods are difficult to reach the detection limit of the existing methods, which has become another major technical bottleneck

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