Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nocardia rubra-cell wall skeleton solvent extraction method

A technology of Nocardia rubrum and extraction method, which is applied in the field of microbial pharmaceuticals, can solve problems such as destruction, personnel damage to the environment, and hidden dangers left by residues, and achieve the effects of ensuring product quality, reducing extraction time, and reducing residues

Active Publication Date: 2017-10-10
FUJIAN SHANHE PHARMA
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the solvent extraction process is: "ethanol, ether, chloroform, methanol separate or mixed step-by-step extraction", the methanol, ether and chloroform used in this process are poisonous and harmful organic solvents, and there may be There are trace residues that leave hidden dangers, and may cause harm to personnel and damage to the environment during the operation process, and further improvement is needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nocardia rubra-cell wall skeleton solvent extraction method
  • Nocardia rubra-cell wall skeleton solvent extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0027] (1), ethyl acetate is added in the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 6ml: 1g, to normal temperature, magnetic stirring for 5 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0028] (2), add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir them magnetically for 16 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 6ml: 1g;

[0029] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 6ml: 1g to the precipitated debris obtained in step (2), stir evenl...

Embodiment 2

[0034] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0035] (1), ethyl acetate is added in the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 5ml: 1g, to normal temperature, magnetic stirring for 7 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0036] (2), add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir them magnetically for 15 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 5ml: 1g;

[0037] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 5ml: 1g to the precipitated debris obtained in step (2) and stir ev...

Embodiment 3

[0042] A method for extracting a Nocardia rubra cell wall skeleton solvent, comprising the following steps:

[0043] (1), add ethyl acetate to the cell wall skeleton fragments of Nocardia rubra obtained after enzymolysis according to the ratio of ethyl acetate and Nocardia rubra cell wall skeleton fragments as 8ml: 1g, and stir magnetically at room temperature for 6 Hours, centrifugation: 3136g, 8°C, 20min, discard the supernatant and keep the precipitated debris;

[0044] (2) Add ethyl acetate to the precipitated fragments obtained in step (1) and centrifuge them, stir magnetically for 18 hours at room temperature, centrifuge: 3136g, 8°C, 20min, discard the supernatant, and leave the precipitated fragments, wherein, The ratio of ethyl acetate to the precipitated fragments obtained in step (1) is 8ml: 1g;

[0045] (3) Add absolute ethanol to the precipitated debris obtained in step (2) at a ratio of 8ml: 1g to the precipitated debris obtained in step (2) and stir evenly, wash...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a nocardia rubra-cell wall skeleton solvent extraction method which is characterized by including the steps: (1) ethyl acetate extraction: adding ethyl acetate in nocardia rubra-cell wall skeleton fragments acquired after enzymolysis, stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (2) absolute ethyl alcohol stirring and cleaning: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (1) to uniformly stir mixture, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (3) absolute ethyl alcohol extraction: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (2), stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, and abandoning liquid supernatant to obtain final sedimentation fragments. The proportion of the nocardia rubra-cell wall skeleton fragments and ethyl acetate is 1g: 5-8ml, and the proportion of the sedimentation fragments acquired in the step (2) and the absolute ethyl alcohol is 1g:5-8ml.

Description

technical field [0001] The invention belongs to the technical field of microbial pharmacy, and in particular relates to a solvent extraction method for the cell wall skeleton of Nocardia rubrum. Background technique [0002] Nocardia rubra (Nocardia rubra) is an immunologically active microorganism. It obtains its cell wall skeleton through cell crushing, removal of cell contents, proteins and lipids and other components. The active substances of neutral polysaccharides and mucopeptides can stimulate cellular immune function, promote the secretion of various cytokines, participate in the anti-tumor immune process, and are used for the treatment or adjuvant therapy of various tumors; they can also be used for the development of new dosage forms and new adaptations Diseases such as skin lesions and gastrointestinal diseases. [0003] In the prior art, the solvent extraction process is: "ethanol, ether, chloroform, methanol separate or mixed step-by-step extraction", the metha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P37/04A61P35/00
CPCA61K35/74
Inventor 徐镜黄春玉龚丽萍黄建
Owner FUJIAN SHANHE PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com