Marine fish peptide powder, preparation method and applications thereof

A technology for marine fish and peptide powder, applied in application, food preparation, animal protein processing and other directions, can solve the problems of poor production technology and product quality, small production scale, low industrial concentration, etc., and achieve easy control of the enzymatic hydrolysis process. , improve functional properties, improve the effect of nutritional composition

Inactive Publication Date: 2014-10-29
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although it is a big producer of fishmeal, it is difficult to call it a strong producer. There are many small regional enterprises in my...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use degreased marine fish meal as raw material, mix evenly with water according to 1:10 ratio of material to water, at 121°C, 1.05kg / cm 2 Cook under pressure for 25 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9, use alkaline protease Alcalase 2.4L (Novozymes, Denmark) as the enzymatic enzyme, control the concentration ratio of enzyme and substrate to 1%, carry out enzymatic hydrolysis at 60°C, and keep it in the enzyme During the hydrolysis process, the pH is stable. After the enzymolysis is completed, heat up to 92°C, inactivate for 10 minutes, add 2g / L activated carbon, keep warm and stir constantly, decolorize and deodorize. 12% of the product, spray-dried, the drying conditions are inlet temperature 150 ° C, outlet temperature 80 ° C, that is.

[0027] After testing, in the marine fish peptide powder prepared in this example, the oligopeptide content is 75.50%, and the relative molecular weight of the oligopeptide powder is...

Embodiment 2

[0029] Use degreased marine fish meal as raw material, mix evenly with water according to the ratio of 1:5 material to water, at 121℃, 1.05kg / cm 2 Cook under pressure for 20 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 7.0, use flavor protease (Novozymes, Denmark) as the enzymolysis enzyme, control the concentration ratio of enzyme and substrate to 2.0%, carry out enzymolysis at 55°C, and keep the pH during the enzymolysis process Stable, after the end of enzymatic hydrolysis, heat up to 90°C, inactivate for 10 minutes, add 4g / L activated carbon, keep warm and stir continuously, decolorize and deodorize, then enter the plate and frame filter press, take the press filtrate and concentrate to 10% solids, Spray drying, the drying conditions are inlet temperature 180°C, outlet temperature 90°C, to obtain.

[0030] After testing, the marine fish peptide powder prepared in this example contains 62.00% of the oligopeptide powder, and the rela...

Embodiment 3

[0032] Use degreased marine fish meal as raw material, mix evenly with water according to 1: 12.5 ratio of material to water, at 130°C, 1.75kg / cm 2 Cook under pressure for 30 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9, use alkaline protease (Wuxi Genencor Bioengineering Co., Ltd.) The pH is stable during the process. After the enzymatic hydrolysis, heat up to 95°C, inactivate for 10 minutes, add 2g / L activated carbon, keep warm and stir constantly, decolorize and deodorize, then enter the plate and frame filter press, and take the filtrate and concentrate it to a solid 10%, spray drying, the drying conditions are inlet temperature 110 ℃, outlet temperature 70 ℃, that is.

[0033] After testing, the oligopeptide content of the marine fish peptide powder prepared in this example is 59.00%, and the relative molecular weight of the oligopeptide powder is lower than 2080 Da, which is a high-quality marine fish peptide powder.

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Abstract

The invention provides a preparation method of fish meal protein peptide. The preparation method comprises the following steps: (1) taking degreased marine fish meal as the raw material, dissolving the fish meal in water according to a weight/volume ratio of 1:5-12.5; (2) boiling for 20 to 30 minutes at a temperature of 115 to 130 DEG C under a pressure of 0.75 to 1.75 kg/cm2, cooling so as to obtain a boiled liquid; (3) adjusting the pH value of the boiled liquid to 7.0-9.5, adding protease, controlling the mass concentration ratio of the protease to the substrate to be 0.5 to 2.5%, carrying out enzymolysis at a temperature of 45 to 70 DEG C so as to obtain enzymatic hydrolysate; (4) heating the enzymatic hydrolysate to 90 to 95 DEG C to inactivate the enzymes, adding active carbon, maintaining the temperature, continuously stirring at the same time so as to carry out decoloring and deodorization, then transporting the processed enzymatic hydrolysate into a plate-and-frame filter press to carry out filter pressing, condensing the clarified filtrate, and finally spray-drying so as to obtain the fish meal protein peptide. The prepared fish meal protein peptide is rich in nutrients, is easy to digest and absorb, and can strengthen the immunity. The nutrition value and biological utilization rate of low-market-value marine fishes are prominently improved. The protein peptide is suitable for preparation of various kinds of functional healthcare foods or nutrition foods.

Description

technical field [0001] The invention belongs to the technical field of marine fish deep processing, and in particular relates to a marine fish peptide powder and a preparation method and application thereof. Background technique [0002] Fishmeal is a high-protein powder obtained from one or more types of marine fish, especially small non-edible fish as raw materials, after a series of processes such as cooking, pressing, dehydration and crushing. Fishmeal is the most commonly used protein source in aquafeeds due to its high quality. Fishmeal has become the most commonly used and most important animal in aquatic feed due to its rich content of essential amino acids, balanced amino acid composition, high protein content, good palatability, high digestibility, high unsaturated fatty acids and low anti-nutritional factors. sex protein source. Protein is one of the most critical nutrients in animal growth. The protein obtained by animals from food is decomposed into amino aci...

Claims

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Application Information

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IPC IPC(8): A23J3/04A23J3/34A23L1/30
Inventor 王立梅沈芳齐斌朱益波
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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