Modified fish skin collagen and preparation method

A fish skin collagen and modification technology, applied in the field of food packaging and processing, can solve the problems of poor mechanical properties and rheological properties of fish skin collagen, and achieve the effects of overcoming poor mechanical properties, simple process and improving gel strength

Inactive Publication Date: 2010-11-24
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of modified fish skin collagen and preparation method thereof, to make up for the deficiencies in the prior art, the gel strength, elasticity and viscosity of the modified

Method used

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  • Modified fish skin collagen and preparation method
  • Modified fish skin collagen and preparation method
  • Modified fish skin collagen and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Prepare fish skin collagen (collagen content above 95%, molecular weight about 3 million Daltons) into a 6.67% (g / ml) aqueous solution, place it at room temperature until the collagen fully swells, and then place it in a water bath at 40°C for 30 minutes When the collagen is fully dissolved, then add transglutaminase (in an amount of 2% of the weight of the collagen in the solution) to the collagen solution, place it in a water bath at 40°C and heat until the transglutaminase is fully dissolved, then take the solution Mix with 2% chitosan acetic acid solution (85% degree of deacetylation, molecular weight 200,000 Daltons) in a ratio of 1:1, homogenize for 5 minutes, vacuum degassing for 5 minutes, and then place in a water bath at 40°C for 30 minutes to heat the two Fully cross-linked to obtain the modified fish skin collagen solution. The prepared modified collagen solution was characterized by texture analyzer and rheometer. The gel strength was 80g, the elastic modul...

Embodiment 2

[0015] Fish skin collagen (collagen content above 95%, molecular weight about 3 million Daltons) is prepared into a 6.67% aqueous solution, placed at room temperature until the collagen is fully swollen, and then heated in a 45°C water bath until the collagen is fully dissolved. Then add glutamine transaminase (addition amount is 2.5% of collagen protein weight in the solution) to this collagen protein solution, place 45 ℃ of water baths and heat until glutamine transaminase fully dissolves, take this solution and 2% chitosan Sugar acetic acid solution (91% degree of deacetylation, molecular weight 500,000 Daltons) was mixed at a ratio of 1:1, homogenized for 5 minutes, vacuum degassed for 5 minutes, and then heated in a water bath at 45°C for 30 minutes until the two were fully cross-linked to obtain Modified fish skin collagen solution. The modified collagen solution was characterized by texture analyzer and rheometer. The gel strength was 90g, the elastic modulus (25°C) was...

Embodiment 3

[0017] Fish skin collagen (collagen content above 95%, molecular weight about 3 million Daltons) is formulated into a 6.67% aqueous solution, placed at room temperature until the collagen is fully swollen, and then heated in a 50°C water bath until the collagen is fully dissolved. Then add glutamine transaminase (addition amount is 3% of collagen protein weight in the solution) in this collagen protein solution, place 50 ℃ of water baths and heat until glutamine transaminase fully dissolves, get this solution and 2% chitosan Sugar acetic acid solution (91% degree of deacetylation, molecular weight 500,000 Daltons) was mixed at a ratio of 2:1, homogenized for 5 minutes, vacuum degassed for 5 minutes, and then heated in a water bath at 50°C for 20 minutes until the two were fully cross-linked to obtain Modified fish skin collagen solution. The modified collagen solution was characterized by texture analyzer and rheometer. The gel strength was 87g, the elastic modulus (25°C) was ...

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Abstract

The invention relates to modified fish skin collagen and a preparation method. The preparation method comprises the following steps of: preparing 6.67 percent aqueous solution from the fish skin collagen in a weight volume ratio of g/ml, then adding glutamine transaminase in an amount which accounts for 2 percent of the weight of the fish skin collagen in the aqueous solution; after dissolving the glutamine transaminase fully, adding 2 percent solution of chitosan acetic acid in the volume ratio of 1-2:1; and homogenizing and performing vacuum degassing, and then heating the mixture at the temperature of between 40 and 50 DEG C for 30 minutes to obtain the modified fish skin collagen solution. The gel strength of the prepared modified fish skin collagen solution is 80 to 90g, modulus of elasticity is 550 to 740MPa at the temperature of 25 DEG C, and modulus of viscosity is 3,160 to 4,230MPa at the temperature of 25 DEG C. In the invention, the fish skin collagen is modified by combining the glutamine transaminase with the chitosan, so that the gel strength of the fish skin collagen solution is intensified, and the defect of low mechanical property is overcome. The modified fish skin collagen has a simple process and good modification effect and can be used for manufacturing packaging films in the fields of food, medicaments and cosmetic.

Description

technical field [0001] The invention belongs to the field of food packaging and processing, and in particular relates to a modified fish skin collagen and a preparation method. Background technique [0002] Collagen is the most abundant protein in animals. Due to its special structure and function, collagen has been widely used in medicine, food and cosmetics and other fields. In recent years, with the deterioration of the global ecological environment, as well as the occurrence of mad cow disease, foot-and-mouth disease and other epidemics, the extraction of collagen from the skin and bone of land animals such as pigs and cattle has been restricted, while fish skin collagen has gradually been subject to more focus on. Compared with terrestrial animal collagen, the content of proline and hydroxyproline in fish skin collagen is relatively low, which leads to its poor rheology, low strength and poor thermal stability, which limits its further application in the field of food ...

Claims

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Application Information

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IPC IPC(8): C08L89/06C08L5/08B65D65/38
Inventor 曾名湧刘尊英葛晓军赵元晖董士远
Owner OCEAN UNIV OF CHINA
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