Method for extracting total DNA of microorganism in liquor Daqu

Abstract

The invention relates to a method for extracting total DNA of microorganism in liquor Daqu, which is characterized in that, the liquor Daqu is pre-treated before extracting DNA, and the pre-treatment comprises the following steps: adding pre-treatment liquid into Daqu: the phosphoric acid buffer solution contains 0.05-0.2w / v% PVPP (polyvinylpolypyrrolidone) and 3-5w / v% tween-80 or tween-60, pH is8.0, wherein the weight volume ratio between Daqu and pre-treatment liquid is 3-8:25; shaking; executing ultrasound treatment; centrifuging at low speed after standing; centrifuging at a low speed after standing; removing supernatant, and adding the phosphoric acid buffer solution containing 0.05-0.2w / v% PVPP (polyvinylpolypyrrolidone) into the deposition; centrifuging at a high speed after scatter, removing supernatant for obtaining sample. The method provided by the invention is simple.

Description

technical field

[0001] The invention relates to a method for extracting microbial total DNA, in particular to a method for extracting microbial total DNA from liquor Daqu. Background technique

[0002] Chinese liquor Daqu is a saccharification and fermentation agent for the production of liquor. Although the predecessors have done a lot of research on the analysis of Daqu microorganisms, the detection and classification methods of bacteria based on morphological and physiological indicators are relatively complicated, and the results obtained The conclusion inevitably deviates from the actual situation. Using the traditional laboratory isolation and culture method, due to the complexity of the environment and the limitation of culture conditions, it is believed that the microorganisms that can be cultured by the existing technology only account for about 1% of the total number of microorganisms, and the traditional laboratory isolation and culture method cannot be timely It...

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