Breeding method capable of controlling aberration rate and body color abnormity of verasper variegatus seedlings
A technology of deformity and deformity of star flounder, which is applied in the field of aquatic seed breeding, can solve the problems of abnormal body color and high deformity rate of star flounder seed, and achieve high survival rate, simple and easy strengthening operation, and high seed quality. Good results
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[0014] Describe technical content of the present invention in detail below by embodiment.
[0015] 1. Strengthen the choice of time
[0016] Put the harvested rotifers into a rotifer strengthening bucket with filtered seawater. The strengthening density of rotifers is 200-300 / ml. Add it into the strengthening barrel, and the strengthening dose is 0.1g / L for 9 hours. Afterwards, selenium-containing yeast (1000 mg / kg selenium content) was added to the fortified water body at a dose of 0.003 g per million rotifers, for 1.5, 3 and 4.5 hours respectively, and three samples were taken at each time point. During the strengthening period, it should be fully inflated, and the dissolved oxygen should not be lower than 5 mg / L. After the strengthening, the collected rotifers were washed with clean sea water, filtered through a 300-mesh sieve to remove excess water, put into a 5ml centrifuge tube, and immediately placed in a -20°C cryopreservation until the content of selenium in the rot...
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