Method for inducing metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow

A methoprene and cascade technology, applied in the field of aquaculture, can solve the problems such as the mechanism of action of juvenile hormone that is far from being completely revealed, and achieve the effects of remarkable induction effect, stable effect and simple operation.

Inactive Publication Date: 2019-11-08
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of research has been done on the molecular mechanism of juvenile hormone in recent years, the mechanism of action of juvenile hormone is far from being fully revealed

Method used

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  • Method for inducing metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow
  • Method for inducing metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow
  • Method for inducing metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. The preparation of the mutagen for the attached metamorphosis of the tortoise cycad:

[0034] According to the weight-to-volume ratio of methoprene: dimethyl sulfoxide of 1:45-60 (w / v), add ethanol to prepare a mutagen containing 8 mg / mL of methoprene, at 4°C Store at low temperature.

[0035] 2. Cascade cultivation pool:

[0036] The structure of the cascade cultivation pool described in this embodiment is shown in the technical scheme. in:

[0037] The size of pool A (2) in the step pool is: 1000mm (length) x 1500mm (width) x 1000mm (height), and the water injection height is 800mm.

[0038]The size of the B pool (4) in the step pool is: 1200mm (length) × 1500mm (width) × 900mm (height), and the water injection height is 700mm.

[0039] The size of pool C (6) in the step pool is: 800mm (length) × 1500mm (width) × 800mm (height), and the water injection height is 600mm. After the seawater in the C pond (6) is full, the tail water is discharged by the draft pipe...

Embodiment 2

[0049] 1. The preparation of the mutagen for the attached metamorphosis of the tortoise cycad:

[0050] According to the weight-to-volume ratio of methoprene: dimethyl sulfoxide of 1:45 (w / v), add ethanol to prepare a mutagen containing 8 mg / mL of methoprene, and store it at low temperature at 4°C .

[0051] 2. Cascade cultivation pool structure:

[0052] The structure of the cascade cultivation pool described in this embodiment is shown in the technical scheme. in:

[0053] The dimensions of pool A (2) in the step pools are: 1 meter (length)×1.5 meters (width)×1 meter (height), and the water injection height is 800mm.

[0054] The size of pool B (4) in the step pool is: 1.2 meters (length) × 1.5 meters (width) × 0.9 meters (height), and the water injection height is 0.7 meters.

[0055] The size of pool C (6) in the step pool is: 0.8 meters (length) × 1.5 meters (width) × 0.8 meters (height), and the water injection height is 0.6 meters. After the seawater in the C pond ...

Embodiment 3

[0065] 1. The preparation of the mutagen for the attached metamorphosis of the tortoise cycad:

[0066] According to the weight-to-volume ratio of methoprene: dimethyl sulfoxide of 1:45 (w / v), add ethanol to prepare a mutagen containing 8 mg / mL of methoprene, and store it at low temperature at 4°C .

[0067] 2. Cascade cultivation pool structure:

[0068] The structure of the cascade cultivation pool described in this embodiment is shown in the technical scheme. in:

[0069] The size of pool A (2) in the step pool is: 1 meter (length)×1 meter (width)×1 meter (height), the water injection height is 0.8 meters, and the volume of the water body is 1 cubic meter.

[0070] The dimensions of pool B (4) in the step pools are: 1 meter (length) × 1 meter (width) × 0.9 meters (height), and the water injection height is 0.7 meters.

[0071] The size of pool C (6) in the step pool is: 0.8 meters (length) × 1 meter (width) × 0.8 meters (height), and the water injection height is 0.6 me...

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Abstract

The invention relates to a method for inducing settlement and metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow. According to the method, firstly, dissolutionis promoted according to the weight volume ratio of 1:(45-60) (w / v) of kinoprene to dimethyl sulfoxide, and ethanol is added to prepare mutagenic agents containing 5-8mg / mL kinoprene. After a pond A and a pond B of a stepped culture pond are filled with clean sea water, the cypris larvae are transferred into the sea water in the pond A and the pond B of the stepped culture pond, and continuous micro-inflation is performed. Afterwards, a mutagenic agent dripping pipe is opened to continuously drip the mutagenic agents to the pond A. A water feeding pump of the stepped culture pond is started toinject water, and continuous water injection time is 4-6 days. 4-6 days after, water injection and dripping are stopped, cypris larva settlement bases are taken out, and then the cypris larvae can betransferred into a larva breeding stage. The method is simple in operation, less in energy consumption, low in cost, stable in function and remarkable in inducing effect, metamorphosis rate can reach80% or more, and the method is a good method for inducing settlement and metamorphosis of the capitulum mitella cypris larvae.

Description

technical field [0001] The present invention relates to the technical field of aquaculture, in particular to a method for inducing the attachment and metamorphosis of the cymbal cymbal larvae by the dropwise addition method of methoprene (CAS: 42588-37-4) Background technique [0002] Turtle foot (Capitulum mitella Linnaeus) is a mariculture species with high economic value and development prospects, belonging to Arthropoda, Crustacea, Cirripedes, Peritus, Stems, and Armorophyta , Refers to the genus Capitulum of the family Pollicipidae. Turtle feet are commonly known as pen holders, bergamot, dog claw snails, etc., and generally live in rock crevices in the middle and high tide areas where the waves are violently impacting and the water flow is smooth. Adhesive to rock or other surfaces. Due to the constraints of the habitat environment, the population of tortoise feet is small and the size of individuals is mixed, and the larvae are often attached to the stalk of the adu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59A01K63/00A01K63/04A01K63/06
CPCA01K61/59A01K63/003A01K63/06A01K63/042A01K63/04Y02A40/81
Inventor 饶小珍林岗
Owner FUJIAN NORMAL UNIV
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