Saline-alkaline tolerant molecular marker C1480 of portunus trituberculatus and application thereof

Abstract

The invention provides a saline-alkaline tolerant molecular marker C1480 for portunus trituberculatus and an application of the saline-alkaline tolerant molecular marker C1480. The nucleotide sequenceof the molecular marker C1480 is as shown in SEQ ID No. 1, and the nucleotide sequence of a primer pair for detecting the molecular marker C1480 is as shown in SEQ ID No. 2 and SEQ ID No. 3. The molecular marker C1480 is an SNP marker, and the alkali-resistant genotype of the molecular marker C1480 is a GG genotype. The molecular marker C1480 provided by the invention is not limited by the growthstage of portunus trituberculatus; the molecular marker C1480 can be used for obviously accelerating the breeding process of portunus trituberculatus and quickly breeding the portunus trituberculatusspecies with excellent saline-alkaline tolerance, is accurate and reliable in detection of the saline-alkaline tolerance of the portunus trituberculatus by using the molecular marker C1480, is simpleto operate, and is beneficial to healthy breeding and development of the portunus trituberculatus, thereby having wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers of aquatic animals, and in particular relates to a saline-alkali-resistant molecular marker C1480 of Portunus trituberculatus and its application. Background technique [0002] Swimming crab (Portunus trituberculatus) belongs to Crustacea, Decapod order, Portunidae, commonly known as swimming crab, is an important large-scale marine economic crab in my country. Swimming crab meat is delicious and nutritious, and is deeply loved by consumers. Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of this type of water resources to a large extent. The physiology and biochemistry of Portunu...

AI Technical Summary

Problems solved by technology

Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of such water resources to a large extent.

The physiology and biochemistry of Portunus trituberculatus will be affected to a certain extent under the stimulation of high salinity, and high salinity will reduce the feeding rate, metamorphosis rate and survival rate of young crabs

However, the trait of salt-alkaline tolerance has obvious low heritability characteristics, and the genetic progress through traditional breeding methods is slow. It is urgent to use advanced molecular marker-assisted breeding technology to speed up the breeding process, and the identification and innovative application of salt-alkali tolerance molecular markers It is the necessary prerequisite and way to carry out molecular marker assisted breeding

[0003] At present, there are few studies on the development of molecular markers for salt-alkali tolerance in Portunus trituberculatus, and there is a lack of markers that can be applied to molecular marker-assisted breeding in the industry

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