Method for detection of protein-protein interaction through a BRET technology based on bacterial Luciferase
A bacterial luciferase, technical detection technology, applied in the field of protein interaction detection based on bacterial luciferase BRET technology, can solve the problems of unsatisfactory detection, low detection accuracy, complicated operation, etc., and achieve convenient detection of protein interaction, Improve the reaction rate, solve the effect of complex operation
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[0031] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0032] figure 1 The flow chart of the method for detecting protein interaction based on bacterial luciferase BRET provided by the present invention is shown. For ease of illustration, only the parts relevant to the present invention are shown.
[0033] The embodiment of the present invention is a method for detecting protein interaction based on bacterial luciferase BRET technology. The method for detecting protein interaction based on bacterial luciferase BRET technology includes the following steps:
[0034] Step 1, construct and clone pBluescript-pLac::luxAB-MCS;
[0035] Step 2, the receptor protein is selected from ...
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