The invention discloses a detection module and detection method for an intelligent production line system for a pipette tip, and belongs to the technical field of pipette tip detection. The detection module comprises a framework, carrying units and a detection unit, the number of carrying units is two, the carrying units are arranged at the two ends of the framework correspondingly, the detection unit comprises a detection sliding table for bearing the pipette tip and a visual detection device for detecting defects of the pipette tip, the visual detection device is fixedly connected to the framework through a fixing frame, the detection sliding table is arranged on the framework in a sliding mode, and the detection sliding table drives the pipette tip to pass through the visual detection device. According to the detection module and the detection method, manual operation can be replaced, pollution in the placement process of the pipette tip is avoided, the placement precision is high, inclination is avoided, placement and removal of the pipette tip are both automatically operated, meanwhile, a PLC can judge good products and defective products in time according to video images of the detection device, in addition, the accurate position is given, and the detection efficiency is high.