CaO2@DOX@ZIF-67 nanometer material and preparation method and application thereof

A nanomaterial and nanotechnology, applied in the fields of nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of difficult application, complicated preparation method, and difficult to degrade, and achieve the effect of improving activity.

Inactive Publication Date: 2019-08-30
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But IL-DOX-PCM-CuO@ZrO 2 The preparation method of the complex is complicated, and ZrO 2 The carrier is not easy to degrade in the body, so it is difficult to realize the application

Method used

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  • CaO2@DOX@ZIF-67 nanometer material and preparation method and application thereof
  • CaO2@DOX@ZIF-67 nanometer material and preparation method and application thereof
  • CaO2@DOX@ZIF-67 nanometer material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] CaO 2 Synthesis of @DOX

[0037] Add 40mL methanol and 50mg CaO to a 100mL round bottom flask 2 , after ultrasonically dispersed evenly, add 10 mL of doxorubicin (DOX) / methanol solution with a concentration of 1 mg / mL, magnetically stir for 3 h, centrifuge and wash with methanol three times to obtain CaO 2 @DOX, dispersed in 2mL methanol for later use.

[0038] CaO 2 Synthesis of @DOX@ZIF-67

[0039] 10mg Co(NO 3 ) 6H 2 O was dissolved in 80 mL of methanol, and the above 2 mL of CaO was added under stirring 2After the methanol solution of @DOX is uniformly dispersed, add 20 mL of 2-methylimidazole / methanol solution with a concentration of 0.6 mg / mL, stir magnetically for 2 h (speed 200 rpm), stop stirring, let stand for 12 h, and centrifuge separated, washed three times with methanol, and dried under vacuum at 60°C for 4 h to obtain CaO 2 @DOX @ZIF-67 nanomaterials.

[0040] For the obtained CaO 2 Structural characterization of @DOX@ZIF-67 nanomaterials, the r...

Embodiment 2

[0047] CaO 2 The synthesis of @DOX is the same as in Example 1.

[0048] CaO 2 Synthesis of @DOX@ZIF-67:

[0049] 10mg Co(NO 3 ) 6H 2 O was dissolved in 80 mL of methanol, and the above 2 mL of CaO was added under stirring 2 After the methanol solution of @DOX is uniformly dispersed, add 20 mL of 2-methylimidazole / methanol solution with a concentration of 0.14 mg / mL, stir magnetically for 2 h (speed 200 rpm), stop stirring, let stand for 12 h, and centrifuge separated, washed three times with methanol, and dried under vacuum at 60°C for 4 h to obtain CaO 2 @DOX @ZIF-67 nanomaterials.

Embodiment 3

[0051] CaO 2 The synthesis of @DOX is the same as in Example 1.

[0052] CaO 2 Synthesis of @DOX@ZIF-67:

[0053] 10mg Co(NO 3 ) 6H 2 O was dissolved in 80 mL of methanol, and the above 2 mL of CaO was added under stirring 2 After the methanol solution of @DOX is uniformly dispersed, add 20 mL of 2-methylimidazole / methanol solution with a concentration of 0.70 mg / mL, stir magnetically for 2 h (speed 200 rpm), stop stirring, let stand for 12 h, and centrifuge separated, washed three times with methanol, and dried under vacuum at 60°C for 4 h to obtain CaO 2 @DOX @ZIF-67 nanomaterials.

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Abstract

The invention provides a CaO2@DOX@ZIF-67 nanometer material and a preparation method and application thereof. The nanometer material is prepared through the steps of loading adriamycin on the surfaceof nanometer CaO2 through coordination effects, then adsorbing metal ions through the adriamycin on the surface of the CaO2, and then enabling the metal ions and bridging ligands to be subjected to anin situ reaction. After a nanometer reactor CaO2@DOX@ZIF-67 is transported to a tumor position through a blood circulating system, through EPR effects, the nanometer reactor CaO2@DOX@ZIF-67 is enriched in tumor tissue and taken in by tumor cells, ZIF-67 is slowly decomposed to release out Co<2+> in subacid environment, besides, CaO2@DOX is decomposed to release out DOX, and then CaO2 and water are subjected to a reaction to generate O2 and H2O2, so that the oxygen concentration of the tumor tissue can be increased, tumor hypoxia is overcome, and sensibility of hypoxia tumor cells to chemotherapy medicines is strengthened. The Co<2+> or Fe<3+> can catalyze H2O2 to generate a Fenton like reaction, massive .OH can be generated, tumor cell DNA can be destructed through oxidization, and deathof the tumor cells is promoted.

Description

technical field [0001] The present invention relates to a nanometer material and its preparation method and application, in particular to a CaO 2 @DOX@ZIF-67 nanomaterials and their preparation methods and applications. Background technique [0002] Doxorubicin (DOX) is a commonly used clinical anti-tumor drug, which can inhibit the synthesis of DNA and RNA, has the strongest inhibitory effect on RNA, has a wide anti-tumor spectrum, and has effects on a variety of tumors. Drugs have a killing effect on tumor cells in various growth cycles. However, due to the drug resistance of tumor hypoxia, the therapeutic effect of doxorubicin is greatly reduced. [0003] Researchers at home and abroad have done a lot of research on reversing tumor hypoxia and improving the effect of DOX chemotherapy. [0004] Zhang Xianzheng et al. used ZIF-8 to load DOX and catalase. Under acidic conditions, ZIF-8 was decomposed to release DOX and catalase, and catalase catalyzed the H overexpressed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/24A61K31/704A61K33/08A61P35/00B82Y5/00
CPCA61K31/704A61K33/08A61K33/24A61P35/00B82Y5/00A61K2300/00
Inventor 高书涛葛昆李振华刘会芳张金超
Owner HEBEI UNIVERSITY
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