97results about How to "Improve the effect of chemotherapy" patented technology

The invention relates to a polydopamine-coated nanometer composite photothermal reagent and a preparation method thereof and belongs to the technical field of function materials. The method is simple to operate and two coating methods are provided: firstly, inorganic nanometer particles or the surface of an assembly of the inorganic nanometer particles are directly coated with a polydopamine shell layer through the oxidative polymerization character of a dopamine monomer; and secondly, nanometer particles are synthesized and coated by one step by utilizing the reducibility and the oxidative polymerization character of dopamine, so that the trouble of synthesizing the nanometer particles in advance is prevented, operation is more simple, and a synthesis method has better green environmental protection character. Proved by experiments, after the nanometer particles are coated with the polydopamine shell layer, the biocompatibility, the physiological environment stability and the photothermal stability of the nanometer particles are greatly improved; by introducing a hydroxyl functional group and an amino functional group, the condition of further modifying a biological targeting matter or a loaded medicine is supplied for the nanometer particles; and in addition, as the polydopamine has the photothermal character, the photothermal conversion efficiency of a material coated with the polydopamine shell layer is improved.

The invention provides a nano-medicinal carrier with magnetocaloric effect. The nano-medicinal carrier with grain diameter of 50-200 nanometers comprises mesoporous silica particles and Fe3O4 nano-particles embedded inside the mesoporous silica particles, wherein the mesoporous diameter of the mesoporous silica particles is 2-10 nanometers, and the mesoporous channel is in a radial shape from the inner core to outside; the diameter of the Fe3O4 particles is 15-20 nanometers. The invention further provides a preparation method of the nano-medicinal carrier and application of the nano-medicinal carrier in loading anti-cancer medicaments. The nano-medicinal carrier is high in drug storage capacity, and can be used for remarkably improving the anti-cancer drug high-performance transmission efficiency, remarkably improving the curative effect of tumor treatment, and realizing cancer therapy by virtue of medical chemotherapy and magnetocaloric therapy.

Apigenin is a nontoxic compound. The present invention is appropriate for apigenin use in people who have a high risk of getting cancer, and in people who have cancer through chemoprevention and chemotherapy, respectively. We showed that apigenin inhibited cancer cell proliferation, tumor growth and angiogenesis. Apigenin selectively inhibited proliferation and induced apoptosis of cancer cells, enhanced the sensitivity of different cancer cells to different therapeutic drugs including cisplatin and taxol. Apigenin also inhibits angiogenesis and tumor growth in human cancers, and inhibits angiogenic inducers such as hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF). Apigenin inhibited expression of HIF-1 and VEGF through PI3K, AKT, p70S6K1 and HDM2 pathways, which are commonly observed in all kinds of human cancers. Thus, our results indicate that apigenin can be applied to various human cancers for chemoprevention, and for chemotherapy when combined with other therapeutic reagents.

The invention discloses a kit detecting hot spot mutation sites in a colorectal cancer PIK3CA gene. The kit comprises an erythrocyte lysate, a whole blood DNA extraction reagent, an anhydrous ethanol, a PCR amplification reaction solution, a positive control, a negative control and a pyrosequencing reaction solution. The kit is characterized by comprising upstream and downstream primers 9F-Biotin and 9R for detecting exon 9 of the target gene and a sequencing primer 9RCX, and upstream and downstream primers 20F-Biotin and 20R for detecting exon 20 of the target gene and a sequencing primer 20RCX. The kit can be used to detect polymorphism of hot spot mutation sites (E542K, E545K and H1047R) in the colorectal cancer related PIK3CA gene, and has good specificity and high accuracy.

The invention discloses application of cysteamine in preparing a medicine for treating cancer. Sensitivity on cancer cells by chemotherapeutics is increased by the cysteamine with low dosage, thereby realizing that cancer cells are effectively killed by the chemotherapeutics with low dosage. The invention greatly improves the medicine effect of the chemotherapeutics and lowers the side effects of chemotherapy.

The invention relates to a medicine for treating malignant tumors, in particular to an L-palmitoyl ascorbate antitumor medicine applied in the way of intravenous injection or oral administration or intratumor injection or the like. The pharmaceutically acceptable medicine applied in an injection or oral administration dosage form is prepared from L-palmitoyl ascorbate or/and one or more kinds of other chemotherapeutic medicine in combination. The medicine has high physicochemical stability and a definite antitumor effect; in-vitro cell tests and tumor-bearing mouse tests show that the medicine or a composition thereof has an effect on various malignant tumors such as breast cancer, liver cancer, lung cancer, stomach cancer, pancreatic cancer, cervical cancer, gliomas, prostatic cancer and colorectal cancer.

The present invention discloses preparation process of medicine-carrying particle containing surface transferrin for glioma targeting chemotherapy, and belongs to the field of preparing technology of medicine-carrying targeting particle. Biodegradable polymer polylactic acid, polyglycolic acid, polycaprolactone or copolymer of lactic acid and glycolic acid and chemotherapeutical medicine carmustine, Adriamycin or taxol are dissolved in acetone, acetonitrile or dimethyl sulfoxide; and the solution is emulsified in solution of transferrin or combined with transferrin chemically after co-dialysis with cholesterol modified glucosan dialdehyde to prepare the medicine-carrying polymer particle containing surface transferrin. This kind of particle may be injected into tumor cavity to make medicine released crossing blood brain barrier and targeting glioma to reach the high inside inhibition of tumor. The present invention has simple preparation process and obvious curative effect.

The invention relates to a synthesis and gluing method of bi-component drug micromolecule hydrogel precursors, which includes connecting an anti-inflammatory agent hexadecadrol and taxol through short peptide to form micromolecule hydrogel precursor molecules, and phosphate buffer solution (PBS) (pH=7.4) of the precursor molecules cuts off disulfide bonds under the effect of glutathione, dithiothreitol or the like to form hydrogel containing two drug components. The drug transmission system effectively overcomes the shortcoming of poor water solubility of the taxol, and the hexadecadrol serving as synergic drug of the taxol can effectively reduce toxic and side effects of the taxol.

The invention relates to a cell membrane coated ultra-small ferroferric oxide nanocluster, and a preparation method and an application thereof. The method comprises the following steps: preparation ofultra-small ferroferric oxide nanoparticles, preparation of surface aminated ultra-small ferroferric oxide nanoparticles, preparation of ferroferric oxide nanoclusters, preparation of adriamycin-loaded ferroferric oxide nanoclusters, preparation of a cell suspension, and preparation of cell membrane coated ultra-small ferroferric oxide nanoclusters. After the nanocluster provided by the inventionis injected into a mouse body through a tail vein, T1/T2 bimodal MR imaging conversion can be achieved; meanwhile, the anti-tumor effect can be exerted; the chemotherapy effect of the nanocluster canbe further enhanced through a UTMD auxiliary means; and the nanocluster has potential clinical application value.

The invention discloses a tumor-targeted photodynamic medicine carrying nanoparticle as well as a preparation method and an application thereof, belonging to a medical preparation containing porphyrin or a medical preparation prepared by a method which utilizes wave energy to process materials. According to the invention, porphyrin or derivates of porphyrin of a functional group and polyethylene glycol and polypropylene glycol of a polyether chain segment are connected through chemical bonds or ester bonds or amido bonds of perssads, and then polymerized into a nanoparticle aqueous dispersion of porphyrin or derivates, loaded with a chemotherapy drug with a conjugated structure and dialyzed to obtain the tumor-targeted photodynamic medicine carrying nanoparticle. The nano material prepared by the method is high in yield, regular in shape, uniform in distribution and good in biosecurity, can be effectively loaded with an antitumor drug with a conjugated structure; a water-soluble photosensitizer can effectively reverse drug tolerance of a chemotherapy drug after being irradiated by near-infrared light; the nanoparticle can efficiently target and position tumors, has good anelasticity, effectively inhibits tumor growth, and has a wide application prospect in preparing breast cancer tumor-targeted drugs.

The invention discloses local chemotherapy equipment for tumors. The local chemotherapy equipment comprises a needle cylinder and a liquid medicine box, wherein a piston is slidably mounted in the needle cylinder; an injection port is formed in the front end of the needle cylinder; a liquid inlet is formed in one side of the front end of the needle cylinder; the top surface of the piston is fixedly connected with a push-pull rod; the top end of the push-pull rod extends to the outer side of the needle cylinder; the top of the liquid medicine box is provided with a liquid adding opening and a sealing cover installed on the liquid adding opening in a sealed mode; the bottom of the liquid medicine box is provided with a liquid outlet; an electric heating pipe is installed in the liquid medicine box; the liquid outlet is communicated with the liquid inlet through a liquid conveying catheter; the injection port is connected with an injection catheter; and the tail end of the injection catheter is connected with a puncture needle. According to the invention, chemotherapy is carried out on tumors through fixed-point puncture injection, so targeted local chemotherapy can be carried out onthe tumors, the side effects of tumor chemotherapy are reduced, and the comfort degree of a patient during chemotherapy is improved; and chemotherapy liquid medicine is stored and heated through the liquid medicine box, and chemotherapy effect is improved through the chemotherapy medicine with physical energy heating effect.

The invention discloses a double targeting liposome with 4-amino-benzene-alpha-D-mannopyranoside (MAN) and wheat germ agglutinin (WGA) modifiers and a preparation method thereof and application. The double targeting liposome is composed of a liposome and modifiers on the surface of the liposome. The modifiers on the surface of the liposome are 4-amino-benzene-alpha-D-mannopyranoside and wheat germ agglutinin. The invention further provides a medicine carrying liposome which is obtained by using a targeting liposome to perform entrapment of medicines. The medicines can concretely be epirubicin hydrochloride and/or resveratrol. According to the double targeting liposome, double ligands are adopted to perform modification and entrapment of the two medicines, the double targeting effect is achieved, so that the medicines are targeted to the glioma part when striding the blood-brain barrier and are enriched at the tumor position; meanwhile, the two medicines in entrapment can have synergistic or additive curative effect on the focus position, and the curative effect of chemotherapy is improved. A novel idea is provided for preparing medicines for treating glioma, and the double targeting liposome is of great theoretical significance and clinical significance.

The invention relates to a visual microvesicle composition carrying adriamycin. The composition is formed by connecting dual-ligand acid-sensitive adriamycin prodrug groups to the surface of a biotinylated lipid microvesicle through an avidin intensive bridging manner, wherein the chemical structure of each dual-ligand acid-sensitive adriamycin prodrug group is shown as a following formula (I). The composition can promote adriamycin release in a tumor acidic microenvironment, can enhance a targeting function, and can exert a function of real-time ultrasonic tumor targeting imaging.

The invention relates to antitumor application of chlorambucil-polydopamine prodrug nanoparticles, and concretely relates to a mild photothermal therapy-chemotherapy combined tumor alete treatment technology based on the chlorambucil-polydopamine prodrug nanoparticles. The nanoparticles can realize spatiotemporal manipulation in an antitumor effect in order to achieve accurately-positioned tumor ablation, and passive targeting action of the prodrug nanoparticles on tumor parts can be achieved by the enhanced permeation and retention(EPR) effect; the mild photothermal effect can enhance the expansion of tumor blood vessels and the permeability of cell membrane effectively promote the accumulation and penetration of the nanoparticles in tumors and the endocytosis of tumor cells; and photothermal therapy can induce the antitumor immunity in order to improve the effect of chemotherapy, so synergistic antitumor effects are generated in the photothermotherapy-chemotherapy combined therapy. The application provides a new way for precise cancer treatment, and has a potential clinical application prospect.

The invention relates to a manganese dioxide and doxorubicin supported nanometer hydrogel and preparation and application thereof. The manganese dioxide and doxorubicin supported nanometr hydrogel isprepared by supporting manganese dioxide and drugs to poly-N-vinyl-epsilon-caprolactam nano hydrogel. The preparation method is simple, the product is easy to purify, and the cost is low. The nanometer hydrogel prepared by using the method is low in particle diameter, is uniformly distributed, and is high in water solubility, colloidal stability and biocompatibility. The nanometer hydrogel prepared by using the method can be used as a magnetic resonance imaging contrast agent, in the combination of an ultrasound-targeted microbubble destruction technology, proliferation of cancer cells can besignificantly inhibited, and the manganese dioxide and doxorubicin supported nanometer hydrogel has a potential application value in the field of tumor diagnosis and treatment.

The invention provides a CaO2@DOX@ZIF-67 nanometer material and a preparation method and application thereof. The nanometer material is prepared through the steps of loading adriamycin on the surfaceof nanometer CaO2 through coordination effects, then adsorbing metal ions through the adriamycin on the surface of the CaO2, and then enabling the metal ions and bridging ligands to be subjected to anin situ reaction. After a nanometer reactor CaO2@DOX@ZIF-67 is transported to a tumor position through a blood circulating system, through EPR effects, the nanometer reactor CaO2@DOX@ZIF-67 is enriched in tumor tissue and taken in by tumor cells, ZIF-67 is slowly decomposed to release out Co<2+> in subacid environment, besides, CaO2@DOX is decomposed to release out DOX, and then CaO2 and water are subjected to a reaction to generate O2 and H2O2, so that the oxygen concentration of the tumor tissue can be increased, tumor hypoxia is overcome, and sensibility of hypoxia tumor cells to chemotherapy medicines is strengthened. The Co<2+> or Fe<3+> can catalyze H2O2 to generate a Fenton like reaction, massive .OH can be generated, tumor cell DNA can be destructed through oxidization, and deathof the tumor cells is promoted.

The invention relates to taxol-based small-molecule hydrogel and a preparation method thereof. The preparation method of the taxol-based small-molecule hydrogel comprises the following steps of: (1) dissolving taxol derivatives obtained by ester bond connection of taxol and hydrophilic compounds through hydroxyl groups into an aqueous medium, wherein the quality of the taxol derivatives dissolved in 1ml of aqueous medium is 5-200mg; and (2) hydrolyzing ester bonds for connecting the taxol and the hydrophilic compounds to obtain taxol, thereby forming the taxol-based small-molecule hydrogel. The degree of hydrolyzing can be defined according to specific conditions so long as the hydrogel can be formed. The invention has the following beneficial effects: the synthesis method is simple and only needs 1-2 reaction steps, and the normal saline or buffer solution of the taxol derivatives forms the small-molecule hydrogel capable of being injected through self-hydrolyzing of the ester bonds; and secondly, a slow-release system takes drug molecules as carriers, and the hydrogel can be locally injected to tumor parts, thereby improving the chemotherapy effect.

The invention relates to application of demethylzeylasteral to preparation of a medicine for treating pancreatic cancer. The application proves that the compound demethylzeylasteral has a significant killing effect on human pancreatic cancer cells, can induce tumor cell cycle arrest in a G0/G1 phase and achieves a pancreatic cancer resisting effect through induction of autophagic death and Caspase-3-dependent apoptosis of the cells; in combination with gemcitabine, the demethylzeylasteral can significantly reduce the IC50 of the gemcitabine, and through combined drug administration, a better inhibitory effect on the human pancreatic cancer cells is achieved; at low concentration, the demethylzeylasteral can induce the autophagic death of the cells so as to improve the antitumor effect of the gemcitabine, while at high concentration, the demethylzeylasteral improves the chemotherapeutic effect of the gemcitabine mainly through promotion of the apoptosis. On the basis of the application, the demethylzeylasteral can be applied to preparation of the medicine for treating the pancreatic cancer, and the prepared medicine is combined with the gemcitabine for use, or the demethylzeylasteral can be used for researching a cell autophagy or apoptosis mechanism.

The invention relates to a high molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and a preparation method thereof. The high molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer mainly comprises a dendrimer PAMAM capable of carrying the pharmacologically activesubstances, and an aldehyde polysaccharide capable of realizing targeting adhesion onto bladder cancer. The invention also provides a high molecular composite gel which is invented based on the high molecular gel and is used for targeting treatment of bladder cancer. Preferably, the high molecular composite gel is loaded with gold nanorods and gemcitabine. The high molecular composite gel possesses following advantages: 1, targeting adhesion onto bladder cancer can be realized, medicine slow release is achieved, medicine effect lasting time is prolonged, the residual amount of medicine in normal bladder epithelial cells is low, and side effect is low; 2, photothermal therapy of bladder cancer is realized, and tumor tissues can be killed through photothermal ablation; and 3, chemotherapy combined thermotherapy is realized, wherein hyperthermia chemotherapy is adopted to promote the killing effect of chemotherapy on tumor tissues. Compared with conventional hyperthermia chemotherapy equipment, the advantages are that: equipment requirement is low, and popularization is convenient.

The invention provides an organic selenium composition which comprises selenium-enriched edible fungus powder, wolfberries, honeysuckle and vitamin E. The organic selenium composition can be used as a food, can also be used as a medicine, can be taken for a long period of time, and is capable of improving the human immunity, preventing tumors, inhibiting growth of cancer cells and synthesis of DNA, RNA and protein, inhibiting oncogene transcription and interfering with metabolism of carcinogenic substances. The selenium-enriched edible fungus powder is matched with the wolfberries, the honeysuckle, the vitamin E and Jerusalem artichoke for use, so that the synergistic effects can be developed, the human immunity is enhanced, especially, the immunity of patients receiving tumor chemotherapy is enhanced, and the chemotherapy effect is strengthened.