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Anti-tumor drug and application of isoniazid in preparation of anti-tumor drug

An anti-tumor drug, isoniazid technology, applied in the field of biomedicine, can solve the problems of weak inhibitory effect on tumor cells, poor effect of anti-tumor drugs, and damage to normal cells, so as to improve the effect of chemotherapy, ensure drug efficacy, and reduce The effect of lethality

Inactive Publication Date: 2019-08-02
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention solves the technical problems of poor antitumor drug killing effect on tumor cells and high toxicity and side effects in the prior art
In view of the above defects or improvement needs of the prior art, the present invention uses isoniazid as an enhancer to prepare antitumor drugs, which solves the problem that the existing chemotherapeutic drugs have a weak inhibitory effect on tumor cells, and normal cells are affected due to poor selectivity. loss of technical issues

Method used

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  • Anti-tumor drug and application of isoniazid in preparation of anti-tumor drug

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Embodiment 1

[0029] Firstly, isoniazid was prepared as a stock solution of 1 mol / L, and diluted to 10 mM with DMEM culture solution containing 5% fetal bovine serum before adding the drug, ensuring that the volume occupied by double distilled water did not exceed 0.5%. like figure 1 , is the group without any treatment as the control group, isoniazid 10mM and doxorubicin 0.1μM single action group, and isoniazid 10mM and doxorubicin 0.1μM combination group (isoniazid The mass ratio to doxorubicin is 25230:1) cell viability.

[0030] In terms of cell treatment, the cells in the logarithmic growth phase were taken, and 5×10 3 Cells / well were inoculated in a 96-well plate, and the total volume of culture medium in each well was 100 μl. After the cells grew for 24 hours, they were given different drug treatment schemes. The cells were cultured for 24 hours, and after 24 hours, the cell viability was detected by sulforhodamine B (SRB method). see results figure 1 . The horizontal axis repre...

Embodiment 2

[0032] The concentration of reactive oxygen species in cells is closely related to survival outcomes such as apoptosis. figure 2 Among them, DOX means doxorubicin, the dose concentration is 0.1 μM; INH means isoniazid, the concentration is 10 mM; DOX+INH means 0.1 μM doxorubicin combined with 10 mM isoniazid (isoniazid and doxorubicin The mass ratio of the star is 25230:1). After 2 hours of drug intervention, the fluorescent probe DCFH-DA was used to detect the changes in the concentration of intracellular reactive oxygen species ROS. figure 2Among them, in the doxorubicin alone group, the intracellular ROS of the two cells increased, and there was no statistically significant difference between the cell lines; in the isoniazid alone group, the intracellular ROS of the two cells increased, and The increase of intracellular ROS concentration in HepG2 was significantly higher than that in L02 cells; in the isoniazid and doxorubicin combined group, the increase in intracellula...

Embodiment 3

[0034] The ability of tumor cells to scavenge reactive oxygen species is weaker than that of normal cells. Utilizing this feature, the activity and content of antioxidants can be inhibited, so that cancer cells can further reach or approach the "death threshold" of oxidation levels, and enhance the effect of chemotherapy. Reduced glutathione is the main source of sulfhydryl groups in most living cells, plays an important role in maintaining the proper redox state of protein sulfhydryl groups, and is a key antioxidant in animal cells. In this example, GSH and GSSG detection kits are used to detect the contents of GSH (reduced glutathione) and GSSG (oxidized glutathione), and at the same time, the ratio of intracellular glutathione to oxidized glutathione to test. image 3 It is the reduced glutathione content in HepG2 and L-02 cells of each group in the experiment of 10mM isoniazid combined with 0.1μM doxorubicin (the mass ratio of isoniazid to doxorubicin is 25230:1). Figur...

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Abstract

The invention discloses an anti-tumor drug and application of isoniazid in preparation of the anti-tumor drug, and belongs to the technical field of biomedicine. According to the application of the isoniazid in preparation of the anti-tumor drug, the anti-tumor drug is a compound preparation of a chemotherapy drug and an enhancer thereof, and the isoniazid is used as the enhancer of the chemotherapy drug. The compound preparation clears away a path through promoting oxidation of tumor cells and blocking cell active oxygen, so as to improve the level of the active oxygen in the tumor cells, thereby enhancing the ability of the chemotherapy drug to kill the tumor cells. According to the anti-tumor drug and the application of the isoniazid in preparation of the anti-tumor drug, the usage amount of the anti-tumor chemotherapy drug is effectively reduced under the same curative effect, the inhibitory effect on the tumor cells is stronger and selectivity is realized, that is, normal cells are less damaged, and the toxic and side effects of doxorubicin are effectively reduced; a safer and more effective chemotherapy drug administration method is provided; a relatively high popularizationvalue is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the application of an antitumor drug and isoniazid as an enhancer in the preparation of the antitumor drug to improve the chemotherapy effect of the antitumor drug on cancer cells and reduce side effects. Background technique [0002] Chemotherapy of tumors with chemotherapeutic drugs is one of the main methods of current cancer treatment. Antitumor drugs are widely used in tumor chemotherapy because of their broad anticancer spectrum, strong antitumor effect and definite curative effect. Traditional antineoplastic drugs are prone to drug resistance, and due to poor selectivity, often lead to less than expected curative effect, and even severe heterogeneous reactions, such as bone marrow suppression and cardiotoxicity, which seriously affect the quality of life and compliance of patients. Even life-threatening. Reuse therapeutic agents originally used to tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4409A61K45/06A61K31/704A61K31/722A61K31/136A61P35/00
CPCA61K31/136A61K31/4409A61K31/704A61K31/722A61K45/06A61P35/00A61K2300/00
Inventor 吴志刚董晓琴
Owner HUAZHONG UNIV OF SCI & TECH
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