High molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and preparation method thereof

A polymer gel and pharmacologically active technology, which is applied in the field of polymer composite gel pharmaceutical preparations, can solve the problems that the curative effect of bladder perfusion drugs cannot be maximized, and achieve the effects of prolonging the drug's action time, promoting the killing effect, and easy application

Active Publication Date: 2018-12-04
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By targeting the above two reasons for the recurrence of bladder cancer, the tumor tissue is completely killed, and the problem that the curative effect of bladder infusion drugs cannot be maximized is solved.

Method used

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  • High molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and preparation method thereof
  • High molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and preparation method thereof
  • High molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of polymer gel targeting bladder cancer to deliver pharmacologically active substances

[0035] (1) The G5 generation dendrimer PAMAM was dissolved in deionized water to prepare a solution with a mass fraction of 10%.

[0036] (2) Add COOH-PEG-OH powder to PAMAM solution to make solution A, wherein the mass ratio of COOH-PEG-OH powder to solute PAMAM is 7:1.

[0037] (3) Dissolving the polysaccharide in deionized water to prepare a solution with a concentration of 5% by mass. Potassium periodate powder was added to the polysaccharide solution, reacted overnight and dialyzed to prepare a 5% formylated polysaccharide solution, namely solution B. Wherein the mass ratio of potassium periodate powder to solute polysaccharide is 19:17.

[0038] (4) Mixing equal volumes of solution A and solution B to prepare a polymer gel that can target bladder cancer and deliver pharmacologically active substances.

Embodiment 2

[0039] Example 2: Preparation of polymer gel loaded with gemcitabine.

[0040] The gemcitabine powder was dissolved in the polymer gel prepared in Example 1 to prepare a targeting polymer gel loaded with gemcitabine. The concentration of gemcitabine is 10 mg / ml.

[0041]In order to illustrate that the drug-loaded polymer gel in this example can be administered in sustained release, it is proved by the following experiments:

[0042] Gemcitabine was dissolved in 1ml normal saline solution and polymer gel solution respectively to prepare aqueous solution and gel solution with a concentration of 10mg / ml, which were sealed in 1000DA dialysis bags and put into two dialysis bags containing 500ml normal saline respectively. in a beaker, stirring continuously. Samples were taken at different time points, and the concentration of gemcitabine in normal saline in two beakers was detected by high performance liquid chromatography, and finally the drug release curves of the two were calc...

Embodiment 3

[0043] Example 3: Preparation of nano-gold rod-loaded polymer gel

[0044] Nano-gold rods with a diameter of about 40nm and an absorption peak between 760mm-800nm ​​were prepared by the seed growth method, such as figure 2 . The nano-gold rods were dissolved in the polymer gel prepared in Example 1 to prepare a polymer gel loaded with nano-gold rods at a concentration of 50 μg / ml.

[0045] In order to illustrate that the polymer gel loaded with nano-gold rods in this example has a photothermal effect, it is proved by the following experiments:

[0046] Put 1ml of polymer gel loaded with nano-gold rods at a concentration of 50μg / ml into an EP tube, irradiate them with near-infrared lasers with a wavelength of 785nm at 0.5W, 1W, and 1.5W for 10 minutes, and record them with a thermal imaging camera. Time-temperature curve. The result is as image 3 , the polymer gel loaded with nano-gold rods can reach a temperature above 50°C under the irradiation of a near-infrared laser ...

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Abstract

The invention relates to a high molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer, and a preparation method thereof. The high molecular gel used for bladder irrigation targeting delivering of pharmacologically active substances to bladder cancer mainly comprises a dendrimer PAMAM capable of carrying the pharmacologically activesubstances, and an aldehyde polysaccharide capable of realizing targeting adhesion onto bladder cancer. The invention also provides a high molecular composite gel which is invented based on the high molecular gel and is used for targeting treatment of bladder cancer. Preferably, the high molecular composite gel is loaded with gold nanorods and gemcitabine. The high molecular composite gel possesses following advantages: 1, targeting adhesion onto bladder cancer can be realized, medicine slow release is achieved, medicine effect lasting time is prolonged, the residual amount of medicine in normal bladder epithelial cells is low, and side effect is low; 2, photothermal therapy of bladder cancer is realized, and tumor tissues can be killed through photothermal ablation; and 3, chemotherapy combined thermotherapy is realized, wherein hyperthermia chemotherapy is adopted to promote the killing effect of chemotherapy on tumor tissues. Compared with conventional hyperthermia chemotherapy equipment, the advantages are that: equipment requirement is low, and popularization is convenient.

Description

technical field [0001] The present invention relates to a polymer gel for delivering pharmacologically active substances targeting bladder cancer and a preparation method, and also relates to a polymer composite gel prepared by adding pharmacologically active substances to the polymer gel. Glue pharmaceutical preparations. The invention belongs to the technical field of medicine. Background technique [0002] Vesical infusion drug therapy is currently a routine adjuvant therapy after non-muscle invasive bladder cancer, and its purpose is to kill the tumor cells and tiny residual lesions released during the operation. However, the current drug dosage form for intravesical infusion is a simple drug aqueous solution, and the drug cannot be effectively enriched in the tumor tissue during bladder infusion, and the duration of the drug is short (within two hours), so the curative effect of the drug cannot be maximized. Surviving tumor cells can replant on the bladder wall, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K41/00A61K47/34A61K47/36A61K9/06A61P35/00A61K31/7068
CPCA61K9/0034A61K9/06A61K31/7068A61K41/0052A61K45/00A61K47/34A61K47/36A61P35/00A61K2300/00
Inventor 徐万海郭鹏宇王璐彭锂赵丹凤
Owner HARBIN MEDICAL UNIVERSITY
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