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HIFU biological targeting synergist and preparation method thereof

A synergist and targeting technology, applied in the field of HIFU, can solve the problems of short residence time in the target area and easy damage to surrounding normal tissues, and achieve the effect of prolonging the residence time, improving HIFU targeted ablation of tumors, and high biological safety

Active Publication Date: 2019-05-14
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of synergists such as iodized oil and microbubble contrast agent in improving the curative effect of HIFU has been widely recognized, but there are still problems such as short residence time in the target area and easy damage to surrounding normal tissues.

Method used

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  • HIFU biological targeting synergist and preparation method thereof
  • HIFU biological targeting synergist and preparation method thereof
  • HIFU biological targeting synergist and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of Cationic Lipid Microspheres-Bifidobacteria by Electrostatic Adsorption

[0049] 1. Preparation and characterization of cationic lipid microspheres

[0050]A certain quality of lipid (lipid raw material purchased from Avanti Polar Lipids, Inc, Alabaster, AL, USA) was mixed together, specifically 5 mg DPPC, 2 mg DSPE-PEG (2000)-Amine, 2 mg DC-cholesterol were dissolved in In 10 mL of chloroform, move the solution to a rotary evaporator at 50 °C to remove the organic solvent and form a thin lipid film. After 1 h, the resulting thin lipid film was hydrated in 2 mL of double-distilled water to form a translucent milky white suspension. Then 100 μl PFH (perfluorohexane) was added dropwise to the suspension, and the suspension was emulsified by ultrasonic waves with a power of 130W for 5 minutes (5 seconds on and 5 seconds off) to obtain cationic lipid liquid nanoparticles (ie cationic lipid microspheres). To prepare fluorescent nanodroplets, DiI (1 mg) or Di...

Embodiment 2

[0071] Preparation of PLGA Microspheres-Bifidobacterium by Covalent Bonding Method

[0072] 1. Preparation of PLGA microspheres with activated carboxyl groups on the surface

[0073] Dissolve 40mg of PLGACOOH in 2ml of chloroform, add fluorescent substance DiI, and vibrate for 40s to obtain colostrum. Mix the colostrum with an appropriate amount of 4% PVA aqueous solution (i.e., polyvinyl alcohol aqueous solution) and vibrate again to obtain double emulsion, then add 10ml of 2% isopropanol aqueous solution, stir magnetically for 2 hours at room temperature, centrifuge, rinse with double distilled water for 3-5 Second, the PLGA microspheres with carboxyl groups on the surface and labeled fluorescent substance Dil are prepared; the carboxyl activator carbodiimide (EDC), sulfo-NHS and the PLGA nanoparticles with carboxyl groups on the surface are fully mixed according to the molar ratio of 30:30:1 Incubate the reaction at room temperature for 60 min in MES buffer at pH 5.5 to ac...

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Abstract

The invention provides a HIFU biological targeting synergist and a preparation method thereof. The HIFU biological targeting synergist is specifically formed by combining bifidobacterium and a HIFU synergist. The bifidobacterium is a probiotic beneficial to the human health and high in biosafety, the obligative anaerobic characteristic of the bifidobacterium can target a tumor hypoxia area, the bifidobacterium is directionally proliferated in the tumor hypoxia area, and the bifidobacterium has an anti-tumor effect. The HIFU biological targeting synergist can more accurately target tumor tissue, and can improve the HIFU energy deposition and prolong the residence time, thereby achieving the purpose of improving HIFU targeted tumor ablation.

Description

technical field [0001] The invention relates to the field of HIFU, in particular to a HIFU biological targeting synergist and a preparation method thereof. Background technique [0002] In recent years, HIFU (High Intensity Focused Ultrasound, High Intensity Focused Ultrasound), as a new type of minimally invasive treatment method, has been widely used in clinical tumor treatment. HIFU uses the directionality, penetrability and focusability of ultrasound to focus the ultrasound emitted outside the body on the target tissue. Through the thermal effect, cavitation effect and mechanical effect of ultrasound, a transient high temperature is generated in the focal area to cause the target tissue to Irreversible coagulation necrosis. Its notable feature is that it can directly kill and destroy the target tissue without damaging the surrounding normal tissue. It is a new technology for non-invasive treatment of malignant solid tumors. However, in actual clinical work, HIFU techno...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/46A61K47/34A61K47/24A61K47/28A61P35/00
Inventor 邹建中杜永洪罗勇熊洁徐蝶蒋冰蕾高悬杨海燕
Owner CHONGQING MEDICAL UNIVERSITY
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