A kind of HIFU biological targeting synergist and preparation method thereof
A synergistic and targeted technology, applied in the field of HIFU, can solve the problems of easily damaged surrounding normal tissues and short residence time in the target area, and achieve the effect of improving the targeted ablation of tumors by HIFU, prolonging the residence time, and having high biological safety.
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Embodiment 1
[0048] Preparation of Cationic Lipid Microspheres-Bifidobacteria by Electrostatic Adsorption
[0049] 1. Preparation and characterization of cationic lipid microspheres
[0050]A certain quality of lipid (lipid raw material purchased from Avanti Polar Lipids, Inc, Alabaster, AL, USA) was mixed together, specifically 5 mg DPPC, 2 mg DSPE-PEG (2000)-Amine, 2 mg DC-cholesterol were dissolved in In 10 mL of chloroform, move the solution to a rotary evaporator at 50 °C to remove the organic solvent and form a thin lipid film. After 1 h, the resulting thin lipid film was hydrated in 2 mL of double-distilled water to form a translucent milky white suspension. Then 100 μl PFH (perfluorohexane) was added dropwise to the suspension, and the suspension was emulsified by ultrasonic waves with a power of 130W for 5 minutes (5 seconds on and 5 seconds off) to obtain cationic lipid liquid nanoparticles (ie cationic lipid microspheres). To prepare fluorescent nanodroplets, DiI (1 mg) or Di...
Embodiment 2
[0071] Preparation of PLGA Microspheres-Bifidobacterium by Covalent Bonding Method
[0072] 1. Preparation of PLGA microspheres with activated carboxyl groups on the surface
[0073] Dissolve 40mg of PLGACOOH in 2ml of chloroform, add fluorescent substance DiI, and vibrate for 40s to obtain colostrum. Mix the colostrum with an appropriate amount of 4% PVA aqueous solution (i.e., polyvinyl alcohol aqueous solution) and vibrate again to obtain double emulsion, then add 10ml of 2% isopropanol aqueous solution, stir magnetically for 2 hours at room temperature, centrifuge, rinse with double distilled water for 3-5 Second, the PLGA microspheres with carboxyl groups on the surface and labeled fluorescent substance Dil are prepared; the carboxyl activator carbodiimide (EDC), sulfo-NHS and the PLGA nanoparticles with carboxyl groups on the surface are fully mixed according to the molar ratio of 30:30:1 Incubate the reaction at room temperature for 60 min in MES buffer at pH 5.5 to ac...
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