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Preparation and application of medicine-carrying polydopamine/dendrimer-gold nanoparticles

A technology of polydopamine nano-dendrimer, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and photodisintegration of drugs in the body, and can solve problems that have not been fully explored , to achieve the effect of improving the effect of chemotherapy, reducing drug resistance and good biocompatibility

Active Publication Date: 2019-10-29
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on this, the construction of a nano-scale "vehicle" that incorporates all the above-mentioned functions has great potential, but has not been fully explored

Method used

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  • Preparation and application of medicine-carrying polydopamine/dendrimer-gold nanoparticles
  • Preparation and application of medicine-carrying polydopamine/dendrimer-gold nanoparticles
  • Preparation and application of medicine-carrying polydopamine/dendrimer-gold nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 2.6 mL, 90 mL, and 40 mL of ammonia water, ethanol, and distilled water respectively, and magnetically stir them in a 45°C water bath to fully mix them. After 15 minutes, 10 mL of 50 mg / mL dopamine monomer aqueous solution was added. After reacting for 12 h, centrifuge and purify (16000 rpm, 10 min) to collect polydopamine nanoparticles.

[0046] According to the molar ratio of 1:50, the dendrimer and the chloroauric acid aqueous solution were mixed, stirred and mixed at room temperature for 15 minutes. Then, according to the molar ratio of 1:3, sodium borohydride aqueous solution was added, and the reaction was stirred for 2 hours. After the reaction, the solution was dialyzed for 2 days (6 times, 1 L / time), and freeze-dried to obtain the dendrimer-gold nanoparticles.

[0047] According to the mass ratio of 1:0.5, the polydopamine nanoparticles were slowly added dropwise into the dendrimer-gold nanoparticles, and the reaction solution was 10 mM Tris buffer with ...

Embodiment 2

[0051] Prepare an ethanol solution of doxorubicin hydrochloride, and add it to the aqueous solution of PDA-G5Au-PEG nanoparticles prepared in Example 1 at a mass ratio of 1:1. After stirring and mixing, triethylamine was added. After 24 hours of reaction, the drug-loaded polydopamine / dendrimer-gold (PDA-G5Au-PEG@DOX) composite nanoparticles were obtained by centrifugation and water washing purification.

[0052] UV-Vis spectrum display (attached figure 2 a), There is an obvious absorption peak around 480 nm, indicating the successful loading of doxorubicin. Field emission electron microscope photos show (attached figure 2 b) shows that the morphology of PDA-G5Au-PEG@DOX nanoparticles is relatively uniform. The test results of laser particle size analyzer (DLS) showed the hydrodynamic size and uniformity of the obtained composite nanoparticles. Refer to the attached figure 2 c. The average size of PDA-G5Au-PEG@DOX composite nanoparticles is 219.4 ± 0.2 nm.

Embodiment 3

[0054] Take the PDA-G5Au nanoparticles prepared in Example 1, put them in the buffer solution of pH 7.4 and pH 6.4, place them on a constant temperature shaker at 37°C, and take samples after 0, 6, 12, and 24 hours respectively, and collect the products. PDA-G5Au-PEG@DOX nanoparticles were placed under different conditions (pH7.4 buffer, pH6.4 buffer, 20 mM hydrogen peroxide, 1.75 W / cm 2 laser irradiation), placed in a constant temperature shaker, and samples were taken regularly to study the release of the chemotherapy drug DOX.

[0055] Transmission electron microscope photos show (attached image 3 a), After shaking for 24 hours, the small black particles on the PDA "core" at pH 6.4 were significantly less than those at pH 7.4. This suggests that mildly acidic conditions can promote the release of "satellite" G5Au nanoparticles. Inductively coupled plasma optical emission spectroscopy (ICP-OES) test analysis (attached image 3 b) It was found that under the condition of ...

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Abstract

The invention relates to preparation and application of medicine-carrying polydopamine / dendrimer-gold nanoparticles. The preparation comprises the steps of modifying dendrimer-gold nanoparticles to the surfaces of polydopamine nanoparticles, and performing polyethylene glycol modification and anticancer medicine adriamycin loading. Obtained composite nanoparticles having a core-satellite structurecan be subjected to acid response to release minisize satellites, medicine delivery in deep tumors is realized, and medicine tolerance generated by tumor hypoxia is relieved; and adriamycin is released at a fixedpoint under internal (acid and hydrogen peroxide) and external (near infrared light) stimulation. The obtained composite nanoparticles have favorable biocompatibility, can realize optoacoustic diagnosis and photothermal treatment on tumor locations, and what's more, can realize remarkable restraining effects on tumor growth. Through the adoption of the method disclosed by the invention, the treatment effects of traditional chemical medicines can be notably improved, more comprehensive three-dimensional tumor treatment is realized, and a new thinking is provided for diagnosis and treatment of malignant tumors.

Description

technical field [0001] The invention belongs to the field of preparation of nano-diagnosis and treatment agents for hypoxic tumors, in particular to a preparation method of drug-loaded polydopamine / dendrimer-gold composite nanoparticles with a "nuclear-satellite" structure and its application in hypoxic tumors. Integrated application of diagnosis and treatment in photoacoustic imaging / photothermal therapy / chemotherapy. Background technique [0002] Chemotherapy, as a routine clinical tumor treatment, has been widely used. However, in order to fully exert its drug efficacy, chemical drugs must reach the tumor site. However, due to their unique properties, such as fast blood pool metabolism and poor water solubility, most anticancer drugs are often difficult to achieve good therapeutic effects. In order to improve the therapeutic effect of cancer, studies have shown that designing and building nano "vehicles" to load chemical drugs can significantly improve the effect of che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K41/00A61K9/51A61K47/34A61K47/10A61K31/704A61P35/00
CPCA61K9/5146A61K31/704A61K41/0042A61K41/0052A61K49/225A61P35/00A61K2300/00
Inventor 刘辉王静静谷达冲阳文婷胡洁唐薇
Owner SOUTHWEST UNIV
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