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Method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions

A plant hormone and astaxanthin technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve high promotion and application value, rapid synthesis and efficient accumulation, and simple operation

Active Publication Date: 2013-04-17
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the production process of astaxanthin derived from C. zofingiensis cannot copy the existing production mode and regulation technology of astaxanthin derived from H. pluvialis. A new set of technological process and equipment, this is the task that must be completed before the industrial production of astaxanthin from C. zofingiensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Activation culture: under aseptic conditions, single algae of Chlorella C. zofingiensis are picked and dropped into sterilized Kuhl liquid medium, and then the algae are inoculated into 100ml sterile In a 250ml Erlenmeyer flask of Kuhl's medium, the temperature is 25°C, the rotation speed is 150rpm, and the light intensity is 100μmol m -2 the s -1 Culture in a shaker with continuous light.

[0017] Kuhl medium formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L disodium hydrogen phosphate dodecahydrate, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA , 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L five Copper sulfate water, 0.012mg / L ammonium molybdate tetrahydrate, 1000ml water, pH 6.5.

[0018] 2) Induction culture: Take the algae liquid grown in the exponential growth phase...

Embodiment 2

[0021] 1) Activation culture: Under aseptic conditions, single algae of Chlorella C. zofingiensis are picked and dropped into sterilized Kuhl liquid medium, and then the algae are inoculated into 100ml sterile In a 250ml Erlenmeyer flask of Kuhl medium, at a temperature of 25°C, a rotational speed of 150rpm, and a light intensity of 100μmol m -2 the s -1 Culture in a shaker with continuous light.

[0022] Kuhl medium formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L disodium hydrogen phosphate dodecahydrate, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA , 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L five Copper sulfate water, 0.012mg / L ammonium molybdate tetrahydrate, 1000ml water, pH 6.5.

[0023]2) Induction culture: Take the algae liquid grown in the exponential growth phase aft...

Embodiment 3

[0026] 1) Activation culture: Under aseptic conditions, single algae of Chlorella C. zofingiensis are picked and dropped into sterilized Kuhl liquid medium, and then the algae are inoculated into 100ml sterile In a 250ml Erlenmeyer flask of Kuhl medium, at a temperature of 25°C, a rotational speed of 150rpm, and a light intensity of 100μmol m -2 the s -1 Culture in a shaker with continuous light.

[0027] Kuhl medium formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L disodium hydrogen phosphate dodecahydrate, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA , 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L five Copper sulfate water, 0.012mg / L ammonium molybdate tetrahydrate, 1000ml water, pH 6.5.

[0028] 2) Induction culture: Take the algae liquid grown in the exponential growth phase af...

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Abstract

The invention belongs to the field of microalgae biotechnologies, and particularly relates to a method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions. The method specifically comprises the steps of: activating and culturing the chlorella vulgaris in a liquid culture medium to obtain a synchronously growing chlorella vulgaris solution for later use; and taking the chlorella vulgaris solution growing to an exponential growth phase, adding the plant hormones and the iron ions in the chlorella vulgaris solution, replenishing a carbon source and a nitrogen source for culturing, ending the culturing until the accumulation of the astaxanthin in chlorella vulgaris cells is maximum (the accumulation of the astaxanthin is determined by adopting a liquid chromatography during sampling), and harvesting cell disruption and extracting the astaxanthin. The method is simple and easy to operate and low in cost, and is capable of remarkably increasing the yield of the astaxanthin, thereby greatly increasing the efficiency of producing the astaxanthin by the chlorella vulgaris.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a method for inducing chlorella to synthesize astaxanthin by utilizing plant hormones and iron ions. Background technique [0002] Astaxanthin is a bright red fat-soluble pigment belonging to ketone carotenoids. A large number of experiments have shown that astaxanthin not only has a unique coloring function, but also exhibits super strong antioxidant properties and various biological activities. Due to its outstanding physiological functions, astaxanthin has broad application prospects in the fields of aquaculture and poultry farming, food, cosmetics, and medicine. Therefore, the production of astaxanthin by various methods has been a hot issue at home and abroad for decades. [0003] At present, the large-scale production of astaxanthin mainly relies on artificial synthesis by chemical means, or from crustaceans and their processed waste, Xanthophyllomyces dendrorhous (...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
Inventor 王艳秦松
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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