Method for extracting astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of medicine, can solve the problems that the freeze-thaw temperature difference method is not very effective, destroys physiological activity, astaxanthin oxidation, etc. The effect of pollution

Inactive Publication Date: 2015-02-04
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used wall-breaking methods include homogenization method, freeze-thaw temperature difference method, direct grinding method, etc. During the homogenization process, high temperature is generated, and the effect of freeze-thaw temperature difference method is not very good. Direct grinding can easily oxidize astaxanthin and destroy its physiology. active

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Seed culture: use BBM medium to expand the culture to the exponential growth phase. Side light is provided by 40W fluorescent lamp, 12:12h lighting, light intensity 1000~1500lx, temperature control 25±1℃. Sampling 15mL of the purchased seed stock solution was placed in a 5L glass instrument with a drainage device for culture, and the expansion and accumulation culture of Haematococcus pluvialis in the later stage was carried out for 10 to 15 days.

[0059] Ventilation and shaking culture: The seeds are cultured in the exponential growth phase, and the cell mass is inoculated on BBM basic medium after centrifugation and cultured for 10-15 days. The culture medium is placed in a ventilated device to expand the culture. Then adopt the stress culture method, use BBM as the basic medium, and set up the nitrogen-deficiency stress medium. In the nutrient stress test, the light intensity is increased to 12000-15000 lx, the temperature is constant, and the culture is cultiv...

Embodiment 2

[0066] (1) Seed culture: use BBM medium to expand the culture to the exponential growth phase. Side light is provided by 40W fluorescent lamp, 12:12h lighting, light intensity 1000~1500lx, temperature control 25±1℃. Sampling 15mL of the purchased seed stock solution was placed in a 5L glass instrument with a drainage device for culture, and the expansion and accumulation culture of Haematococcus pluvialis in the later stage was carried out for 10 to 15 days.

[0067] Ventilation and shaking culture: The seeds are cultured in the exponential growth phase, and the cell mass is inoculated on BBM basic medium after centrifugation and cultured for 10-15 days. The culture medium is placed in a ventilated device to expand the culture. Then adopt the stress cultivation method, use BBM as the basic medium, and set the nitrogen deficiency stress medium. In the nutrient stress test, the light intensity is increased to 12000~15000lx, the temperature is constant, and the culture is cultiv...

Embodiment 3

[0074] (1) Seed culture: use BBM medium to expand the culture to the exponential growth phase. Side light is provided by 40W fluorescent lamp, 12:12h lighting, light intensity 1000~1500lx, temperature control 25±1℃. Sampling 15mL of the purchased seed stock solution was placed in a 5L glass instrument with a drainage device for culture, and the expansion and accumulation culture of Haematococcus pluvialis in the later stage was carried out for 10 to 15 days.

[0075] Ventilation and shaking culture: The seeds are cultured in the exponential growth phase, and the cell mass is inoculated on BBM basic medium after centrifugation and cultured for 10-15 days. The culture medium is placed in a ventilated device to expand the culture. Then adopt the stress culture method, use BBM as the basic medium, and set up the nitrogen-deficiency stress medium. In the nutrient stress test, the light intensity is increased to 12000-15000 lx, the temperature is constant, and the culture is cultiv...

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Abstract

The invention belongs to the technical field of medicines, and relates to a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of culturing a seed stock solution in a glass apparatus with a drainage system, and carrying out later amplification culture of the haematococcus pluvialis and accumulation culture of the astaxanthin after 10-15 d; centrifuging a culture in an exponential growth period of seed culture; and inoculating cell clusters in a BBM basal medium to obtain a primary culture. The later accumulation culture of the astaxanthin is amplification culture by using a breathable plastic bag type simple device provided by the invention. In the accumulation stage, a stress culturing method is adopted to obtain a lab-scale test haematococcus pluvialis culture; and haematococcus pluvialis powder is obtained by spray drying. According to a preparation technology that extracts astaxanthin from the haematococcus pluvialis by adopting an ultrasonic cell disruption assisted mixed solvent extraction method, the haematococcus pluvialis powder is added in an organic solvent to carry out ultrasonic cell disruption, and then the astaxanthin is obtained by the steps of reflux extraction in a water bath, suction filtration, filtrate merging and concentration. Compared with a conventional direct extraction method, the method provided by the invention saves extraction time, and increases astaxanthin yield.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a method for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Astaxanthin is an oxygen-containing derivative of carotenoid, which has strong antioxidant activity, and has many physiological effects such as inhibiting tumorigenesis and enhancing immune function. The market is huge, and it has been widely used in food, medicine, cosmetics and breeding industries. [0003] Haematococcus pluvialis is a single-cell microalgae belonging to Chlorophyta, Volvox, Haematococcus, and Haematococcus. It is recognized as the best biological source of natural astaxanthin in nature. , under excellent culture conditions, the astaxanthin content of Haematococcus pluvialis can reach more than 3%, which is much higher than that of Rhodotorula and other microorganisms. Therefore, the use of Haematococcus pluvialis to extract astaxanthin has broad development Foregrou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 韩静齐计英
Owner SHENYANG PHARMA UNIVERSITY
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