Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus

A technology of Enterobacter ludwigii and Enterobacter ludwigia, which is applied to Enterobacter ludwig BG10-1 and its biological control of Aspergillus flavus, can solve the problems of Aspergillus flavus pollution and achieve pollution prevention and control , the effect of obvious antibacterial activity

Active Publication Date: 2016-05-18
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the objectives of the present invention is to provide a strain of Enterobacter ludwig that has a better control effect on Aspergillus flavus for the problem of Aspergillus flavus contamination in peanuts and the deficiencies of the prior art

Method used

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  • Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus
  • Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus
  • Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus

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Experimental program
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Effect test

Embodiment 1

[0044] Example 1: Preparation of Enterobacter ludwig BG10-1 microbial fertilizer

[0045] The Enterobacter Ludwigii BG10-1 involved in the present invention has been preserved in the China Center for Type Culture Collection (CCTCC) on January 7, 2016, the preservation number is CCTCCNO: M2016014, and the classification name is Enterobacter LudwigiiBG10-1 . It was isolated and screened from peanut pods in Babu Town, Hezhou, Guangxi. Through indoor antagonism experiments and in vivo antivirus experiments, it was found that the strain has strong antibacterial effects, good and stable antibacterial and detoxification effects, easy to cultivate, no pollution, and environmentally friendly. Safety.

[0046] The biological fertilizer preparation method of Enterobacter ludwig BG10-1 bacterial strain is as follows:

[0047] 1) Inoculate Enterobacter ludwigii BG10-1 into the liquid medium for fermentation production. The conditions for fermentation production are: culture temperature 3...

Embodiment 2

[0055] The inhibitory effect of embodiment 2 metabolites to Aspergillus flavus

[0056] Preparation of Metabolites of Biocontrol Bacteria

[0057] After the biocontrol bacteria BG10-1 is activated on the LB slope, take a ring in the LB culture medium, fill in 100mL / 300mL, shake at 37°C and 160r / min for 48 hours, and prepare the following treatment solution: supernatant: The culture solution was centrifuged at 12,000 r / min for 15 minutes, and the supernatant was taken, filtered through a 0.22 μm bacterial filter to obtain a sterile supernatant.

[0058] Bacterial suspension: Centrifuge the culture solution at 12000r / min for 15min, discard the supernatant, wash with sterile water for 3 times and then centrifuge, add sterile water.

[0059] Crude protein extract: centrifuge the culture solution at 12000r / min at 4°C for 15min to discard the precipitate, add solid ammonium sulfate to the supernatant to 70% saturation, let stand overnight at 4°C, centrifuge at 10000r / min at 4°C for...

Embodiment 3

[0066] The colonization test of embodiment 3 biocontrol bacteria

[0067] Preparation of strains labeled with Rif and Nal double antibodies

[0068] Cultivate the BG10-1 strain overnight at 37°C in LB liquid medium, centrifuge at 5000rpm for 2min, collect the bacteria, spread evenly on the LB plate containing Nal antibiotics (final concentration: 50μg / mL), and culture at 35°C . When a single colony resistant to Nal grows on the plate, select a single colony with good growth and inoculate it on the LB plate containing Nal, and transfer it continuously for 3 generations. If the colony grows stably and has little difference from the wild-type strain, Then it was selected as the mutant strain induced to obtain resistance to Nal and named as BG10-1-N.

[0069] Shake-culture the mutant strain BG10-1-N overnight at 35°C on LB liquid medium containing Nal antibiotics (final concentration: 50 μg / mL), centrifuge at 5000 rpm to collect the bacteria, and spread evenly on the medium cont...

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Abstract

The invention relates to enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus. The enterobacter ludwigii BG10-1 is preserved in China Center for Type Culture Collection (CCTCC) on January 7, 2016, the preservation number is CCTCC NO: M 2016014, and the classification name is EnterobacterLudwiggi BG10-1. The enterobacter ludwigii BG10-1 is obtained by being separated from peanut pods produced in Babu Town, Hezhou City, Guangxi Province. Through indoor confrontation and antagonism experiments, living body bacteriostasis and detoxification experiments and field prevention and control experiments, it is found that the strain is high in bacteriostatic capacity on the aspergillus flavus, easy to culture, free of contamination and safe to environment. By applying microbial fertilizer of the enterobacter ludwigii BG10-1, contamination of the aspergillus flavus to peanuts can be effectively prevented and controlled.

Description

technical field [0001] The invention relates to a Ludwig enterobacter BG10-1 strain and its application in biological control of Aspergillus flavus. Background technique [0002] Toxin pollution caused by Aspergillus fungi (especially Aspergillus flavus) is collectively referred to as Aspergillus toxin pollution, and has a wide range of hosts. Aspergillus, especially Aspergillus flavus, has seriously harmed the safe production of peanuts, corn, rice and soybeans, and caused economic losses of tens of billions of dollars every year. Aspergillus, especially Aspergillus flavus, can produce extremely toxic mycotoxins. Aflatoxins are a class of strong carcinogenic and highly toxic mycotoxins that widely contaminate agricultural products, including B, G and M groups, of which B1 is the most common and the most toxic. Human or animal consumption of toxin-contaminated food or feed will lead to disease or even death, which seriously threatens the health and life safety of consumers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F11/08C12R1/01
CPCA01N63/00C05F11/08C12N1/205C12R2001/01
Inventor 李培武姚彦坡丁小霞张奇张文
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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