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Isoflavone compound in tobacco rhizome and preparation method and application thereof

A compound and isoflavone technology are applied in the field of isoflavones and their preparation, and achieve the effects of good antibacterial activity, easy availability of raw materials, and easy separation.

Inactive Publication Date: 2012-02-15
YUNNAN RES INST OF TOBACCO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates an isoflavone compound with antibacterial activity from tobacco rhizomes, and applies it to cigarette antibacterial tipping paper. No related reports have been seen on this compound so far.

Method used

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  • Isoflavone compound in tobacco rhizome and preparation method and application thereof
  • Isoflavone compound in tobacco rhizome and preparation method and application thereof
  • Isoflavone compound in tobacco rhizome and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0027] Tobacco rhizome samples were collected in Yuxi, Yunnan, and the variety was Honghua Dajinyuan. Sampling 1.5-2.5 kg of tobacco rhizome samples was crushed to 20 mesh, extracted three times with 95% ethanol by ultrasonic, 30 minutes each time, the extracts were combined, filtered, and concentrated under reduced pressure to form an extract. The extract was dissolved with an appropriate amount of methanol (1.5 times the weight of the extract), and then mixed with 80-120 g of crude silica gel (80 mesh), and 1.5-2.0 kg of silica gel (100 mesh) was packed into a column for silica gel column chromatography. Chloroform: acetone (20:1 → 1:1) gradient elution, TLC monitoring The same fractions were combined to obtain 5 fractions, among which chloroform-acetone (8:2) eluted fractions were used for Agilent 1100 semi-preparative HPLC Chromatographic separation, using 40% methanol as mobile phase, (20 mm×250 mm, 5 μm) C 18 The preparative column was a stationary phase, the flow rate ...

Embodiment 2

[0029] Tobacco rhizome samples were collected in Yuxi, Yunnan, and the variety was Honghua Dajinyuan. Sampling 1.5-2.5 kg of tobacco rhizome samples was crushed to 30 meshes, extracted four times with 95% ethanol by ultrasonic, 50 minutes each time, the extracts were combined, filtered, and concentrated under reduced pressure to form an extract. The extract was dissolved with an appropriate amount of methanol (1.5 times the weight of the extract), and then mixed with 80-120 g of crude silica gel (90 mesh), and 1.5-2.0 kg of silica gel (160 mesh) was packed into a column for silica gel column chromatography. Chloroform: acetone (20:1 → 1:1) gradient elution, TLC monitoring The same fractions were combined to obtain 5 fractions, among which chloroform-acetone (8:2) eluted fractions were used for Agilent 1100 semi-preparative HPLC Chromatographic separation, using 40% methanol as mobile phase, (20 × 250 mm, 5 μm) C 18 The preparative column was a stationary phase, the flow rate ...

Embodiment 3

[0031] Tobacco rhizome samples were collected in Yuxi, Yunnan, and the variety was Honghua Dajinyuan. Sampling 1.5-2.5 kg of tobacco rhizome samples was crushed to 40 mesh, extracted five times with 95% ethanol by ultrasonic, 60 minutes each time, the extracts were combined, filtered, and concentrated under reduced pressure to form an extract. The extract was dissolved with an appropriate amount of methanol (1.5 times the weight of the extract), and then mixed with 80-120 g of crude silica gel (100 mesh), and 1.5-2.0 kg of silica gel (2000 mesh) was packed into a column for silica gel column chromatography. Chloroform: acetone (20:1 → 1:1) gradient elution, TLC monitoring The same fractions were combined to obtain 5 fractions, among which chloroform-acetone (8:2) eluted fractions were used for Agilent 1100 semi-preparative HPLC Chromatographic separation, using 40% methanol as mobile phase, (20 × 250 mm, 5 μm) C 18 The preparative column was a stationary phase, the flow rate ...

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Abstract

The invention discloses an isoflavone compound in a tobacco rhizome and a preparation method and application thereof. The preparation method comprises the following steps of: crushing a tobacco rhizome sample, performing ultrasonic extraction for 3 to 5 times by using 95 percent ethanol, combining the extracts, filtering, performing reduced pressure concentration to obtain an extract, primarily separating the extract by using silica gel column chromatography, further separating the extract by adopting high performance liquid chromatography, and thus obtaining the required new compound. Antibacterial activity screening test results show that the compound has strong antibacterial effect on common proteus species, escherichia coli, staphylococcus, bacillus subtilis and the like. The compound used for cigarette tipping paper plays an obvious role in inhibiting microbes for polluting the cigarette tipping paper.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry. More specifically, the invention relates to a new isoflavone compound contained in tobacco rhizomes and a preparation method thereof; at the same time, it also relates to the antibacterial effect of the compound and its antibacterial tipping in cigarettes. application in paper. Background technique [0002] Tobacco is one of the most chemical substances among the various plants known to mankind. After decades of research, more than 3,000 individual chemical substances have been identified from tobacco, and many components have not yet been identified. come out. In addition to being mainly used for smoking cigarettes, tobacco can also extract a variety of valuable chemical components from it, and discover leading compounds that are valuable for development and utilization. In particular, the roots and stems of tobacco are generally treated as waste from the tobacco leaf production process. Ther...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40A01N43/16A01P1/00A01P3/00A24B15/26D21H21/36
Inventor 李天飞陈章玉杨光宇陈永宽缪明明谭建林
Owner YUNNAN RES INST OF TOBACCO SCI
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