The invention discloses a preparation method of carboxylated acrylonitrile-butadiene latex for disposable gloves. The preparation method utilizes following raw materials: 30%-50% of butadiene, 10%-25%of methacrylic acid, 20%-30% of acrylonitrile, 15%-20% of a nonionic emulsifier, 5%-15% of deionized water, 1%-5% of a catalyst and 3%-5% of an antioxidant. According to the method, the butadiene, the methacrylic acid, the acrylonitrile and other raw materials are adopted for preparation, the reaction efficiency is high, high strength and small shrinkage are achieved, and the catalyst is preheated and subjected to heat preservation, so that the reaction efficiency is effectively improved, the temperature runaway phenomenon is avoided, the antioxidant is added in a candidate manner, the quality of a finished product is effectively improved, and the oxidative damage is reduced, so that the technological process can be greatly simplified, the control is facilitated, quality of the product isimproved, and popularization and application are facilitated.