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SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes

A technology of high-strength fibers and fiber materials, applied in the field of biotechnology applications, can solve the problems of low reliability of QTL mapping results, less markers, and less research on positioning QTL.

Active Publication Date: 2011-10-19
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the research on cotton fiber QTL mapping based on the intraspecies map of upland cotton has been gradually paid attention to, but there are still problems such as too few markers, low map density, and few studies on multi-environment co-localization of QTL, leading to QTL The reliability of the positioning results is low, and it is difficult to apply molecular marker-assisted breeding

Method used

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  • SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes
  • SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes
  • SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes

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Embodiment Construction

[0020] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0021] (1) Recombinant inbred line F 6:8 the acquisition

[0022] For the field planting and DNA extraction from 2007 to 2008, please refer to the patent application publication number: CN 101613761A, the title of the invention: the patent application document of the SSR marker linked to the main effect gene of cotton fiber strength. In 2009, the parents and F 6:10 group. Anyang and Quzhou adopt a single-row area with a length of 5 meters and a row spacing of 0.8m and (0.8+0.5)m respectively, with 20 plants in each row; Xinjiang adopts a 6-row area with a length of 2 meters and 15 plants in each row. In 2010, the parents and F 611 For groups, Anyang and Zhengzhou use single-track areas with a row length of 5m and a row spacing of 0.8m; Gaoyi adopts a single-row area with a ...

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Abstract

The invention discloses an SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect gene points, namely qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4, qFS-1-5, qFS-9-1, qFS-9-2 and qFS-2-1, wherein qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4 and qFS-1-5 are positioned on c25 chromosomes, qFS-9-1 and qFS-9-2 are positioned on c7 chromosomes, qFS-2-1 is positioned on a c13 chromosome, the marker interlocked with the qFS-1-1 is Gh478350, the marker interlocked with the qFS-1-2 is DPL0387220, HAU892170 and DC40429190, the marker interlocked withthe qFS-1-3 is COT012140, the marker interlocked with the qFS-1-4 is SHIN-1131240, PGML00463450, Gh537190 and PGML00463250, the marker interlocked with the qFS-1-5 is HAU916150, CGR5201200 and CGR564385, the marker interlocked with the qFS-9-1 is CGR5001245, the marker interlocked with the qFS-9-2 is HAU1693150, and the marker interlocked with the qFS-2-1 is SHIN-1452115 and PGML00353240. By utilizing the SSR markers interlocked with the cotton high-strength fibre major-effect genes to carry out auxiliary selection, the efficiency of the cotton fibre quality breeding can be greatly improved.

Description

technical field [0001] The invention relates to an SSR (Simple Sequence Repeats) marker linked with a main effect gene of 0-153 high-strength fiber of cotton high-quality fiber material, and belongs to the field of biotechnology application. Background technique [0002] As a major fiber crop, cotton plays an important role in the world economy. Among the four cotton species widely cultivated in the world, upland cotton occupies the most important position, and its output accounts for more than 90% of the world's total cotton output. With the increase of people's demand for medium and high-grade textiles, quality breeding is becoming more and more important, but it is difficult to improve the quality of cotton fiber by conventional means. The traditional breeding method is mainly based on phenotype selection, the breeding efficiency is low, and it is difficult to meet the needs of quality breeding. The development of molecular marker technology has made it possible to dire...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 袁友禄贾菲孙福鼎李俊文刘爱英石玉真龚举武商海红巩万奎王涛
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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