SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes
A technology of high-strength fibers and fiber materials, applied in the field of biotechnology applications, can solve the problems of low reliability of QTL mapping results, less markers, and less research on positioning QTL.
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[0020] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.
[0021] (1) Recombinant inbred line F 6:8 the acquisition
[0022] For the field planting and DNA extraction from 2007 to 2008, please refer to the patent application publication number: CN 101613761A, the title of the invention: the patent application document of the SSR marker linked to the main effect gene of cotton fiber strength. In 2009, the parents and F 6:10 group. Anyang and Quzhou adopt a single-row area with a length of 5 meters and a row spacing of 0.8m and (0.8+0.5)m respectively, with 20 plants in each row; Xinjiang adopts a 6-row area with a length of 2 meters and 15 plants in each row. In 2010, the parents and F 611 For groups, Anyang and Zhengzhou use single-track areas with a row length of 5m and a row spacing of 0.8m; Gaoyi adopts a single-row area with a ...
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