Listeria monocytogenes nucleic acid chromatography detection kit, its detection method and application thereof

A technology for detecting kits and bacterial nucleic acids, which is applied in the fields of molecular biology and immunology, can solve the problems of long detection time and cumbersome steps, and achieve the effects of low detection cost, simple operation process, and reduced detection cost
CN102520172AInactive Publication Date: 2012-06-27北京陆桥技术股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京陆桥技术股份有限公司
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a listeria monocytogenes nucleic acid chromatography detection kit, its detection method and an application thereof, and belongs to the field of molecular biology and immunology. According to the invention, with the combination of a polymerase chain reaction technology in molecular biology and an immunochromatography technology, rapid detection of listeria monocytogenes in food is accomplished. The kit provided by the invention includes three parts of DNA extraction, PCR amplification and strip detection. The combination of the molecular biology technology and the immunochromatography technology is accomplished by modification of primers and selection of corresponding coatings and markers in strip preparation so as to achieve specific amplification product detection of listeria monocytogenes in food. Compared with a traditional biochemistry method, the method provided by the invention is used to greatly save detection time and simplify operation steps, has characteristics of accuracy, rapidity, convenience and safety, and is suitable for detection of a large number of samples.
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Description

technical field

[0001] The invention belongs to the fields of molecular biology and immunology, and relates to a kit and a method for rapidly detecting Listeria monocytogenes in food. Background technique

[0002] As the country pays more and more attention to food safety and import and export trade, the requirements for food safety testing technology are also getting higher and higher. According to the requirements for the development of food safety and entry-exit inspection and quarantine work in the field of public security in the "National Medium and Long-Term Science and Technology Development Plan (2006-2020)", research on efficient, fast and accurate detection technology has become a topic in this field. One of the main trends and goals.

[0003] According to the information held by the World Health Organization, among the risk factors of foodborne diseases, microbial food poisoning is still the primary hazard; in my country, according to the statistics of the Chines...

Claims

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