Whole-blood immunoassay device and blood analyzer with same

Abstract

The invention provides a device and a method for simultaneously carrying out immune measurement and blood cell measurement on whole blood. The device comprises a reaction tank, a hemolytic reagent supplying part, a sample injection part, an immune reagent supplying part, a light irradiation unit, a control unit, a transmission light detection unit, a scattered light detection unit and an analytical calculation unit, wherein the reaction tank is used for reaction and measurement of a hemolytic agent, an immune reagent and a whole blood test sample; the hemolytic reagent supplying part is used for injecting the hemolytic agent into the reaction tank; the sample injection part is used for injecting the whole blood test sample into the reaction tank to form a first test sample; the immune reagent supplying part is used for adding the immune reagent into the first test sample to form a second test sample; the light irradiation unit provides irradiation light; the control unit is used for controlling the temperature of the reaction tank and controlling the hemolytic reagent supplying part, the sample injection part and the immune reagent supplying part to act according to a certain sequence; the transmission light detection unit is used for detecting absorbance of the first test sample; the scattered light detection unit is used for detecting the strength of scattered light of the second test sample; the analytical calculation unit is used for carrying out analytical calculation on the absorbance and the strength of the scattered light to obtain blood routine parameters and specific protein parameters.

Description

technical field [0001] The present invention relates to a blood analysis technology, which can not only count and analyze blood cells, but also quantitatively measure specific proteins (especially C-reactive protein) in blood plasma by using immunological methods, and this immunological measurement is performed in Performed in whole blood without centrifugation. Background technique [0002] C-reactive protein (C-Reactive protein, referred to as CRP). Discovered as early as 1930, it is an acute phase reaction protein that can react with pneumococcal C polysaccharide to form a complex. The detection of CRP was widely used clinically as a non-specific marker of inflammation and tissue damage before the 1980s. As an acute phase protein, CRP rapidly rises several hours after the onset of various acute inflammations, tissue damage, myocardial infarction, surgical trauma, radiation damage and other diseases, and has a tendency to multiply; when the disease improves, it quickly r...

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Problems solved by technology

[0006] The purpose of the present invention is to solve the clinical problems of comprehensive consideration of CRP detection and blood cell detection results, but the actual operation process requires two different instruments to detect separately, which brings about the disadvantages of increased cost, cumbersome procedures and inconvenient reading of independent test reports ;Invent an instrument and method that can perform CRP detection and blood routine detection at the same time, realize the detection of the two in the same inspection process and combine the reports, which is beneficial to doctors for clinical diagnosis

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