Substrate activation kit and method of detecting DNA or the like by using the same

A kit and substrate technology, applied in the field of detection of DNA or protein, can solve problems such as harmful active sites and difficulties in accurate detection of DNA
CN1606693AInactive Publication Date: 2005-04-13TOYO KOHAN CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TOYO KOHAN CO LTD
Publication Date
2005-04-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The purpose of the present invention is to provide a convenient and fast method for immobilizing DNA on a substrate and a method for accurately detecting DNA, and the density of DNA immobilized by the method is as high as that of the traditional method. A substrate activation kit is provided, which includes phosphate buffered saline at pH 6, solution A (aqueous) of N-hydroxysuccinimide and 1-[3-(dimethylamino)propyl]3-ethylcarbodi Solution B of imine (dioxane solution); a method for detecting DNA is provided, which is characterized in that the DNA is spotted onto the substrate activated with the above-mentioned activation kit, and then hybridized with fluorescently labeled DNA with the spotted DNA.
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Description

technical field

[0001] The present invention relates to a substrate activation kit used in the research of medicine, biochemistry and molecular biology, and also relates to a method for detecting DNA or protein using the kit. Background of the invention

[0002] Genetic analysis is used in the fields of molecular biology and biochemistry, and more recently in medicine, for example in the diagnosis of diseases.

[0003] The recent invention of a substrate that can immobilize DNA has greatly facilitated gene analysis, and the substrate is also used in the medical field to diagnose diseases.

[0004] One method of immobilizing DNA on a substrate is to coat a polymer such as polylysine on the surface of a glass slide or a silicon substrate before immobilization or to synthesize the DNA onto the substrate using semiconductor techniques such as photolithography.

[0005] However, in the method of immobilizing DNA using a polymer such as polylysine, there is a problem of detachmen...

Claims

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