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Preparation method of fibroin-immobilized DNA adsorbent based on glutaraldehyde crosslinking action and application thereof in aflatoxin elimination

A technology of glutaraldehyde cross-linking and aflatoxin, applied in chemical instruments and methods, applications, dairy products, etc., can solve the problem of lack of strength, hardness, toughness, plasticity, inability to build wear-resistant, pressure-resistant, impact-resistant, Fatigue resistance, resistance to bending parts or appliances, DNA practical application limitations and other issues, to achieve the effect of good mechanical properties

Inactive Publication Date: 2019-04-05
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since DNA is a water-soluble biomacromolecule, and lacks appropriate mechanical properties such as strength, hardness, toughness, and plasticity, it cannot be simply separated from the water-soluble reactant or medium after the reaction, nor can it be constructed with a certain wear resistance. Parts or appliances with properties such as pressure resistance, impact resistance, fatigue resistance, and bending resistance. Therefore, the practical application of DNA is still greatly limited.

Method used

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  • Preparation method of fibroin-immobilized DNA adsorbent based on glutaraldehyde crosslinking action and application thereof in aflatoxin elimination
  • Preparation method of fibroin-immobilized DNA adsorbent based on glutaraldehyde crosslinking action and application thereof in aflatoxin elimination

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation of silk fiber immobilized DNA I.

[0023] According to the ratio of 0.8:10 in the mass ratio of natural DNA to silk fiber, natural DNA and silk fiber are added to a phosphate buffer containing 0.25 wt% glutaraldehyde and the pH is 6-8 (the amount of phosphate buffer It is advisable to completely soak natural DNA and silk fiber), mix thoroughly, and react at 25°C for 1 hour. Rinse repeatedly with distilled water after the reaction, and keep the natural fiber in a dispersed state without agglomeration, that is, the silk fiber immobilized DNA Ⅰ, dry it for use, or soak a certain amount of silk fiber immobilized DNA Ⅰ in the buffer solution , short-term refrigerated for later use. The actual DNA content in the prepared dried silk fibroin-immobilized DNA I was 4.5%. The DNA content was determined according to the PicoGreen fluorescent dye method.

Embodiment 2

[0024] Embodiment 2: Preparation of silk fiber immobilized DNA II.

[0025] According to the ratio of 0.5:10 in the mass ratio of natural DNA to silk fiber, add natural DNA and silk fiber to a phosphate buffer solution containing 0.50wt% glutaraldehyde and a pH of 6-8, mix well, and The reaction was carried out at 25°C for 1 hour. Rinse repeatedly with distilled water after the reaction, and maintain the dispersed state of the natural fibers without agglomeration, that is, the silk fibers are immobilized with DNA II and dried for later use, or a certain amount of silk fibers with immobilized DNA II is immersed in a buffer solution , short-term refrigerated for later use. The actual DNA content in the prepared dry silk fiber immobilized DNAⅡ was 3.5%. The DNA content was determined according to the PicoGreen fluorescent dye method.

Embodiment 3

[0026] Example 3: Preparation of Silk Fibroin Immobilized DNA I.

[0027] According to the ratio of natural DNA and silk fibroin mass ratio of 0.8:10, add natural DNA and silk fibroin to a phosphate buffer solution containing 0.25wt% glutaraldehyde and a pH of 6-8, mix well, and The reaction was carried out at 25°C for 1 hour. After the reaction, add methanol (the maximum amount of methanol used is 80% of the total mass of the reaction solution) to promote the coagulation of silk fibroin into a water-insoluble silk fibroin-immobilized DNA I. Isolate the silk fibroin-immobilized DNA I, rinse it repeatedly with distilled water, dry it for use, or soak a certain amount of silk fibroin-immobilized DNA I in a buffer solution, and refrigerate it for short-term use. The actual DNA content in the dried sample was 4.7%. The DNA content was determined according to the PicoGreen fluorescent dye method.

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Abstract

The invention provides a method for preparing novel fibroin-immobilized DNA through chemical crosslinking, especially crosslinking of glutaraldehyde molecule and amino and a method for capturing and eliminating aflatoxin by allowing DNA on the fibroin-immobilized DNA and aflatoxin to be in chelation action and separating and removing the fibroin-immobilized DNA. The adsorbent is especially suitable for eliminating aflatoxin in grease like peanut oil, maize oil and cow's milk and dairy products. The DNA is hydrophilic and soluble in water, thereby being insupportive of separation from water-soluble media or reaction systems and lack of mechanical performance like proper strength, hardness, toughness and plasticity; fibroin fiber is hydrophilic and insoluble in water and is good in mechanical performance, bio-safe and degradable, so that the fibroin-immobilized DNA can avoid original defects of DNA and can fully display and give play to performance of the DNA in capturing and eliminatingaflatoxin.

Description

technical field [0001] The invention relates to a preparation method of a silk fibroin-immobilized DNA adsorbent based on glutaraldehyde crosslinking and its application in eliminating aflatoxin. Background technique [0002] Aflatoxin, mainly secreted by Aspergillus flavus or Aspergillus arasiticus, is currently recognized as the most carcinogenic natural substance, and is classified as Class I by the International Agency for Research on Cancer (IARC) of the World Health Organization Carcinogenic substance with very high hepatotoxicity, tumorigenicity, teratogenicity and mutagenicity. Almost all agricultural products that humans depend on for survival, including grains, peanuts, corn, soybeans, etc., may be caused by the growth of Aspergillus flavus (Aspergillus flavus) or Aspergillus arasiticus (Aspergillus arasiticus), among which peanut and corn are the most likely to breed the corresponding mold, peanut oil and corn oil are most likely to be contaminated by aflatoxin, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C11B3/10A23C7/04
CPCA23C7/04B01J20/24C11B3/10
Inventor 李军生阎柳娟黄国霞
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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