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Gene chip for detecting tumour chemotherapy drug susceptibility

A chemotherapeutic drug and gene chip technology is applied in the field of gene chips for detecting the sensitivity of tumor chemotherapeutic drugs. low effect

Inactive Publication Date: 2007-05-23
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has two biggest disadvantages: First, the amount of specimens that can be provided clinically is often difficult to meet the experimental requirements, and a sufficient number of pure cancer cells cannot be obtained
The second is primary digested tumor cells. Single tumor cells are mixed with agglomerated cells. When the proportion of agglomerated cells is large, it is often impossible to obtain a better purification effect.
However, traditional methods such as Northern, Southern blotting, and Western blotting can only analyze changes in a small number of individual genes

Method used

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  • Gene chip for detecting tumour chemotherapy drug susceptibility
  • Gene chip for detecting tumour chemotherapy drug susceptibility
  • Gene chip for detecting tumour chemotherapy drug susceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] sequence listing

[0028] SEQ ID No.1.5' aatgtggagt cctgtggctc tctgcgtcct gaaaccattg tcctgtcagc cctct3'

[0029] SEQ ID No.2.5' ccgaggaggt ggacctctcc aaggacattc agcactggga atccctgaaa cccga3'

[0030] SEQ ID No.3.5' ttcaaggagc tggaaggctg ggagccagat gacgacccca tagaggaaca taaaa3'

[0031] SEQ ID No.4.5' agtgatgaat tttaacttct ggaaatcaga cttttacaac tggatgtgtg actag3'

[0032] SEQ ID No.5.5' gaggacgtga agctccttcc agacgatgaa aacacgccgg aaaagcgagc cgaga3'

[0033] SEQ ID No.6.5' gggacctggc agcgagaaac attcttttat ctgagaacaa cgtggtgaag atttg3'

[0034] SEQ ID No.7.5' cctggggacg agcggcggaa actcatccga tacgtcacag ccttcaatgg ggagc3'

[0035] SEQ ID No.8.5' gcaggtagag gaggaagttg atgccatgct ggccgtcaag aagtaaagga aagaa3'

[0036] SEQ ID No.9.5' accagagaac tgaccaacga tggggaactg atcctgacca tgacggcgga tgacg3'

[0037] SEQ ID No.10.5' gataaccaga cctgctgccg agacaaccga cagggctggg cctgctgtcc ctacg3'

[0038] SEQ ID No.11.5'accagtccaa cttcagctta tcaggtgccc agatagatga caacaatccc aggcg3'

...

Embodiment 2

[0088] The gene chip for detecting the sensitivity of tumor chemotherapeutic drugs, the DNA probe for detecting drug sensitivity is fixed on the glass slide, and the DNA probe is the sequence table SEQ ID No.1, SEQ ID No.2, SEQ ID No.5, SEQ ID No. The nucleotide sequence composition shown in ID No.9 and SEQ ID No.50.

Embodiment 3

[0090] The gene chip for detecting tumor chemotherapeutic drug sensitivity, the DNA probe for detecting drug sensitivity is immobilized on the silicon chip, and the DNA probe is the sequence list SEQ ID No.10, SEQ ID No.15, SEQ ID No.26, The composition of the nucleotide sequences shown in SEQ ID No.28 and SEQ ID No.55.

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PUM

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Abstract

The invention discloses a gene chip for detecting the sensitivity of tumor chemotherapeutics. The gene chip is to immobilized DNA probes for detecting the sensitivity of drugs on slide glass, silicon chip or polymer material. The said DNA probes comprise at least five nucleotide sequences of SEQ ID No.1 to SEQ ID No.59. The expression level of Tumor chemotherapeutics sensitivity-related gene selected in the invention relates to the sensitivity of organism on drugs. The gene chip is characterized in: detecting the sensitivity or drug fast of organism on drugs on molecular level, high detection flux, high sensitivity, short time, low cost, and simple operation.

Description

technical field [0001] The invention relates to a biological chip, in particular to a gene chip for detecting the sensitivity of tumor chemotherapeutic drugs. Background technique [0002] Although a variety of anticancer drugs are used clinically, the resistance of tumor patients to anticancer drugs seriously restricts the efficacy of clinical chemotherapy. Tumors are insensitive to certain drugs from the very beginning, which is due to the intrinsic characteristics of tumor cells or factors related to drugs; tumors are initially sensitive to chemotherapy, but once relapsed, they present a completely different phenotype, which affects Previously used drugs and new drugs with different structures and different mechanisms of action all develop resistance, also known as multidrug resistance (MDR). The mechanism of drug resistance is multifaceted, and the mechanism of drug resistance is also different. Such as the reduction of drug uptake; the change of intracellular drug dis...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 汤华刘民李欣
Owner TIANJIN MEDICAL UNIV
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