Diluent for preserving porcine semen at normal temperature

A technology for preservation and dilution at room temperature, applied in the preservation, application, animal husbandry, etc. of human or animal body, can solve the problems of high price of imported diluent and inability to guarantee the quality of diluent, and achieves the goal of promoting healthy and sustainable development and reducing labor costs. Insemination production costs, the effect of improving utilization

Active Publication Date: 2010-10-13
兰州天舜畜牧养殖技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of imported diluent is expensive, which is unaffordable for ordinary pig farms
However, the quality of the diluent prepared by ourselves cannot be guaranteed, and the major pig farms still use imported diluent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 5.5g, sucrose 1.5g, fructose 1.6g, lactose 15.0g, trehalose 1.5g, glycine 3.2g, glutamic acid 3.8g g, arginine 1.5g, proline 1.0g, citric acid 1.8g, sodium citrate 3.3g, sodium bicarbonate 2.2g, sodium dihydrogen phosphate 1.2g, tris 4.1g, chloride 0.25g of potassium, 0.32g of magnesium chloride, 0.5g of vitamin E, 0.5g of cysteine, 1.5g of sodium edetate, 0.07g of penicillin, and 0.05g of streptomycin; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.

Embodiment 2

[0018] Embodiment 2, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 3.8g, sucrose 5.0g, fructose 3.2g, lactose 10.0g, trehalose 2.5g, glycine 3.0g, glutamic acid 4.2g g, 2.2g of arginine, 1.2g of proline, 1.2g of citric acid, 3.6g of sodium citrate, 3.7g of sodium bicarbonate, 2.6g of sodium dihydrogen phosphate, 1.7g of trishydroxymethylaminomethane, chloride Potassium 0.35g, magnesium chloride 0.45g, vitamin E 0.35g, cysteine ​​0.45g, sodium edetate 1.0g, penicillin 0.1g, streptomycin 0.07g; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.

Embodiment 3

[0019] Embodiment 3, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 7.3g, sucrose 2.6g, fructose 1.2g, lactose 12.0g, trehalose 1.5g, glycine 3.5g, glutamic acid 3.8g g, arginine 2.2g, proline 0.5g, citric acid 2.2g, sodium citrate 4.1g, sodium bicarbonate 2.5g, sodium dihydrogen phosphate 1.5g, trishydroxymethylaminomethane 2.6g, chloride Potassium 0.43g, magnesium chloride 0.5g, vitamin E 0.3g, cysteine ​​0.2g, sodium edetate 1.2g, penicillin 0.08g, streptomycin 0.06g; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.

[0020] The process of preserving pig semen with the present invention:

[0021] 1. Collection and detection of semen The semen is collected by manual hand-held semen collection on a dummy pig platform. After the semen is collected, it is immediately sent to the laboratory for testing of vitality, viability, density, color, smell and other items.

[0022] 2. Dilution of...

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PUM

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Abstract

The invention relates to a diluent for preserving porcine semen at normal temperature. A total solute of the diluent comprises 45 to 55 percent by weight of monose combination, 15 to 25 percent by weight of amino acid combination and 25 to 35 percent by weight of electrolyte, buffer, antioxidant, stabilizing agent and antibiotics. Various substances are dissolved in double distilled water and a volume of 1, 000 milliliters is fixed so as to finally make an osmotic pressure of the diluent in the range of 330 to 360mosmo/L and make a pH value of the diluent in the range of 6.3 to 6.5. After theporcine semen is preserved at normal temperature in the environment of the diluent of the invention for 5 to 7 days, the viability can still be kept over 0.6 and the acrosom entire rate can reach 85 percent. When the porcine semen preserved by adopting the diluent of the invention is used for mating on 260 sows, the conception rate and the litter size reach 90.2 percent and 10.7 respectively and reach or exceed the levels of the import diluent. The diluent of the invention replaces the import bovine serum albumin with the monose combination and the amino acid combination, but the cost of the diluent of the invention is only 1/3 of that of the import diluent, so the production cost of artificial insemination of swines is reduced greatly.

Description

technical field [0001] The invention relates to an artificially prepared compound diluent for storing pig semen at room temperature, which is mainly used in the production and reproduction of pig raising industry. Background technique [0002] Since the 1950s, our country has carried out in-depth exploration and research on pig semen storage at room temperature and artificial insemination technology. After more than half a century of continuous improvement and development, this technology has become mature. At present, pig semen is stored in two ways: normal temperature storage and low temperature storage. During cryopreservation, due to the low content of phosphatidylcholine in the plasma membrane of porcine sperm, and the high content of phosphatidylethanolamine and sphingomyelin, the distribution of cholesterol is asymmetrical, more on the outside of the plasma membrane, and the ratio of cholesterol / phospholipid is significantly lower than that of other mammals. sperm. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张尤嘉张振韬曲亚玲胡俊杰
Owner 兰州天舜畜牧养殖技术服务有限公司
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