Diluent for preserving porcine semen at normal temperature
A technology for preservation and dilution at room temperature, applied in the preservation, application, animal husbandry, etc. of human or animal body, can solve the problems of high price of imported diluent and inability to guarantee the quality of diluent, and achieves the goal of promoting healthy and sustainable development and reducing labor costs. Insemination production costs, the effect of improving utilization
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Embodiment 1
[0017] Embodiment 1, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 5.5g, sucrose 1.5g, fructose 1.6g, lactose 15.0g, trehalose 1.5g, glycine 3.2g, glutamic acid 3.8g g, arginine 1.5g, proline 1.0g, citric acid 1.8g, sodium citrate 3.3g, sodium bicarbonate 2.2g, sodium dihydrogen phosphate 1.2g, tris 4.1g, chloride 0.25g of potassium, 0.32g of magnesium chloride, 0.5g of vitamin E, 0.5g of cysteine, 1.5g of sodium edetate, 0.07g of penicillin, and 0.05g of streptomycin; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.
Embodiment 2
[0018] Embodiment 2, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 3.8g, sucrose 5.0g, fructose 3.2g, lactose 10.0g, trehalose 2.5g, glycine 3.0g, glutamic acid 4.2g g, 2.2g of arginine, 1.2g of proline, 1.2g of citric acid, 3.6g of sodium citrate, 3.7g of sodium bicarbonate, 2.6g of sodium dihydrogen phosphate, 1.7g of trishydroxymethylaminomethane, chloride Potassium 0.35g, magnesium chloride 0.45g, vitamin E 0.35g, cysteine 0.45g, sodium edetate 1.0g, penicillin 0.1g, streptomycin 0.07g; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.
Embodiment 3
[0019] Embodiment 3, a kind of pig semen storage diluent at room temperature, its solute formula and its weight parts are: glucose 7.3g, sucrose 2.6g, fructose 1.2g, lactose 12.0g, trehalose 1.5g, glycine 3.5g, glutamic acid 3.8g g, arginine 2.2g, proline 0.5g, citric acid 2.2g, sodium citrate 4.1g, sodium bicarbonate 2.5g, sodium dihydrogen phosphate 1.5g, trishydroxymethylaminomethane 2.6g, chloride Potassium 0.43g, magnesium chloride 0.5g, vitamin E 0.3g, cysteine 0.2g, sodium edetate 1.2g, penicillin 0.08g, streptomycin 0.06g; water and dilute to 1000mL, and dissolve in a water bath at 36°C for 1 hour.
[0020] The process of preserving pig semen with the present invention:
[0021] 1. Collection and detection of semen The semen is collected by manual hand-held semen collection on a dummy pig platform. After the semen is collected, it is immediately sent to the laboratory for testing of vitality, viability, density, color, smell and other items.
[0022] 2. Dilution of...
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