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Fluorescent detection probe for mercury ions and application method thereof

A fluorescent probe and fluorescent detection technology, applied in the field of detection, can solve the problems of high cost of DNA and enzymes, and achieve the effect of simple method, no toxic side effects, and high sensitivity

Inactive Publication Date: 2010-05-26
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, nucleic acid aptamers (aptamers) or DNA enzymes have been used as functional reagents to prepare probes with gold nanoparticles for the detection of Hg 2+ [Xue X J, Wang F, Liu X G, J.Am.Chem.Soc.2008, 130, 3244-5; Liu C W, Hsieh Y T, Huang C C, Lin Z H, Chang H T, Chem.Comm.2008, 2242-4 ; Wang H, Wang Y X, Jin J Y, Yang R H, Anal.Chem.2008, 80, 9021-9028; Ye B C, Yin B C, Angew.Chem.Int.Ed.2008, 47, 8386-9; Liu B, Biosensors and Bioelectronics, 2008, 24, 756-760; Yu C J, Cheng TL, Tseng W L, Biosensors and Bioelectronics, 2009, 25, 204-210.] and other heavy metal ions can achieve better sensitivity and selectivity, but due to DNA and enzymes are relatively expensive and cannot be the choice for general application

Method used

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  • Fluorescent detection probe for mercury ions and application method thereof
  • Fluorescent detection probe for mercury ions and application method thereof
  • Fluorescent detection probe for mercury ions and application method thereof

Examples

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Embodiment 1

[0027] Under the condition of a water bath at 38°C, the mass ratio of bovine serum albumin and chloroauric acid two reactants is 1.8:1 to form an aqueous solution, and the two solutions are mixed under magnetic stirring. After 3 minutes, add 5% 1M NaOH solution adjusts the pH value of the mixed solution, and then under constant temperature conditions, continuously stirs for 12 hours to obtain bovine serum albumin functionalized gold nanoparticles (BSA-GNPs), i.e. fluorescent probes, at 365nm Under the light source, the gold nanoparticles functionalized by bovine serum albumin emit red light, and the fluorescence spectrum of the test solution is excited at a wavelength of 470nm, and has a strong emission spectrum at 638-650nm.

Embodiment 2

[0029]In the process of using bovine serum albumin-functionalized gold nanoparticles as fluorescent probes to detect mercury ions in biological tissues or environmental sewage, the prepared fluorescent probes were diluted 40 times, and the diluted fluorescent probes were mixed with biological tissues or environments. The volume ratio of the sewage sample is 1:4 and the probe solution and the sample to be tested are added respectively. For example, take 100 μl of diluted fluorescent probe solution and add it to 400 μl of the sample to be tested, and then observe whether there is red under a 365nm light source Mercury ions greater than 2.5μM will quench the fluorescence. If there is fluorescence, the fluorescence spectrum of the test solution is excited at a wavelength of 470nm, and there is a strong emission peak at 638-655nm. According to the concentration of mercury ions and the fluorescence The linear relationship of intensity can be used to judge whether there is mercury ion...

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Abstract

The invention belongs to the technical field of detection, and particularly relates to a method for detecting heavy metal ions in aqueous solution by using a fluorescent probe. The method, by providing bovine serum albumin functionalized gold nano-particles serving as the fluorescent probe and using the fluorescent probe for detecting mercury ions in a water body, obtains high sensitivity and selectivity. The method utilizes bovine serum albumin modified gold nano-particles as the fluorescent probe; during the detection, various amino and carboxyl groups on the surface of the probe can be coordinated with the heavy metal ions so as to ensure that the fluorescent probe quenches; the initial concentration of the mercury ions and the strength of fluorescence have good linear relation; and the existence of the heavy metal ions, namely mercury in the aqueous solution can be indicated through the fluorescent detection method. The method does not need large-scale instruments, can realize in-situ quick detection, has low detection limit, can be applied to more conditions and ranges, and has simple operation and low cost; and a used reagent and the operation process have no toxic or side effect.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a fluorescent detection probe for mercury ions and an application method thereof, as well as the application of the fluorescent probe for selectively identifying heavy metal ions in an aqueous solution. Background technique [0002] Heavy metal ions (mercury Hg 2+ ; lead Pb 2+ ; Cadmium Cd 2+ ; Copper Cu 2+ ), especially mercury, cadmium, lead, copper, etc. have significant biological toxicity. Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and use of heavy metal products. Heavy metals that exist in various chemical states or chemical forms will persist, accumulate and migrate after entering the environment or ecosystem, causing harm. Heavy metal pollution can cause great harm. For example, public hazards such as Min...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/52
Inventor 柴芳苏忠民王春刚李鹿王婷婷赵亮高洪泽
Owner NORTHEAST NORMAL UNIVERSITY
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