Application of Raman encoding microsphere and method for detecting tumor marker by utilizing Raman encoding microsphere
A tumor marker and Raman coding technology, applied in the field of Raman coding microspheres, can solve the problems of harsh experimental conditions, complicated operation, and weak detection specificity of a single indicator, and achieve the effect of broadening the application scope.
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Embodiment 1
[0033] In this example, taking the tumor marker CEA as an example, the method for detecting the tumor marker CEA by using Raman-encoded microspheres is described.
[0034] (1) Preparation of Raman-encoded microspheres
[0035] a. Take 95mL ultrapure water, add 1mL 30mM sodium citrate solution and 2mL 5mM silver nitrate solution in turn, then quickly inject 1mL 50mM sodium borohydride solution, stir at room temperature for 30 seconds, add 1mL 5mg / mL polyvinylpyrrolidone, the solution Gradually turn into deep yellow, and the silver nanoparticle sol with a diameter of 5-15nm is obtained.
[0036]b. Mix 3.6mL tetraethyl orthosilicate and 88.1mL ethanol into a 250mL round-bottomed flask, quickly add 11.9mL ammonia water under stirring conditions, and make it completely react at room temperature to obtain carbon dioxide with a diameter of about 200nm. Silicon nanoparticles.
[0037] c. Take 25mL of the silica nanoparticles prepared in step b, centrifuge and disperse in 50mL of 2mg...
Embodiment 2
[0048] Example 2: Simultaneous detection of tumor markers AFP, PSA and CA125 by a Raman-encoded microsphere
[0049] (1) Preparation of Raman-encoded microspheres
[0050] The preparation method of the Raman coded microspheres in this example is the same as in Example 1, except that 3-methoxythiophenol, 2-methoxythiophenol and 2-naphthylthiophenol are used to replace p-mercaptoaniline as Raman active substances, respectively obtained encapsulated 3-methoxythiophenol-labeled core-shell nanospheres, encapsulated 2-methoxythiophenol-labeled core-shell nanospheres and encapsulated 2-naphthylthiol Labeled core-shell nanospheres.
[0051] (2) Preparation of Raman-encoded labeled nanoprobes
[0052] Add 125 μg of AFP antibody to 25 mg of the above-prepared encapsulated 3-methoxythiophenol-labeled core-shell nanospheres, react at 4°C for 12 hours, and finally block unreacted aldehydes with a mass concentration of 1% BSA base vacancy, centrifuged and washed to obtain 3-methoxythioph...
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