33results about How to "No biological safety hazard" patented technology

The related bio-reagent is designed by: with conservative African swine fever virus VP73 gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 66bp. This invention is fit to fast testing for African swine fever.

The invention relates to a biopreparate designed and synthesized by applying primer Express and primer prere 5.0 softwares and using the gene fragment of foot and mouth disease virus as a target, especially a reagent able to detect the foot and mouth disease virus and a method of preparing the reagent. The fluorescent quantitative RT-PCR detecting reagent of foot and mouth disease virus contains a pair of specific primers and a specific fluorescent probe. It uses the most conservative fragment of the gene sequence in FMDV 3D region for the first time to design and synthesize the primers and probe, and builds the fluorescent quantitative RT-PCR and prepares a detecting reagent box, and can rapidly detect FMDV in cell culture substance, hydatid fluid, hydatid skin and secretion, blood, and meat products. It creates a fluorescent quantitative RT-PCR of FMDV.

The present invention relates to multifunctional egg powder for a creep feed and a production method thereof. The multifunctional egg powder is characterized by comprising, by weight, 62-68 parts of egg powder, 24-30 parts of soybean separation protein, 3-8 parts of maltodextrin, 2-5 parts of L-lysine, 0.05-0.2 part of lysozyme, and 0.05-0.2 part of immunoglobulin, wherein the components are uniformly mixed, the egg powder comprises, by weight, 50-90% of a chicken whole egg liquid, 5-20% of full fat soybean powder, and 5-30% of soybean oil, uniform stirring and high pressure homogenization are sequentially performed, the homogenization pressure is 5-30 MPa, thermal insulation is performed for 3-10 min at a temperature of 55-70 DEG C to sterilize, and a spraying manner is adopted to rapidly dry to prepare the egg powder. The multifunctional egg powder contains high protein, fat, lecithin, cephalin and other functional ingredients o as to provide easy digestion and utilization effects for piglets, can be added with the lysozyme component and the immunoglobulin component extracted from the egg white so as to effectively replace antibiotics in the piglet creep feed, avoid homology spreading of the plasma protein powder and improve animal growth.

The invention discloses a blood layered identification method, which comprises the steps of: calibrating a sample tube; making a calibration every other set unit volume, recording physical heights of the calibrations and corresponding positions in an image; identifying boundary of blood in the sample tube; and executing a volume calculation step including the processes of calculating volume by adopting a piecewise interpolation algorithm, conducting interpolating calculation when calculating a result according to intervals to which pixel positions belong, acquiring the volume V1 and the pixel position P1 of a first calibration point as well as the volume V2 and the pixel position P2 of a second calibration point from the calibration data, and calculating V3 according to similar triangles when the pixel position of a point to be calculated is P3. The blood layered identification method can utilize 2 or even 4 cameras for shooting 16 samples at one time, and the speed is dozens of times faster than that of laser scanning. By adopting the blood layered identification method, biological safety hazards do not exist, each sample has a picture, and the operator can find out interference easily when encountering the interference.

The invention relates to the technical field of biology, in particular to a reagent which can simultaneously distinguish and detect four animal insect-borne diseases as well as a preparation method and application of the reagent. The animal insect-borne disease multi-RT-PCR distinguishing and detecting reagent comprises four pairs of specific primers, and the respective amplification target fragment lengths of Bluetongue virus (BTV), epizootic haemorrhagic disease virus of deer (EHDV), Vesicular stomatitis virus (VSV) and Akabane virus (AKV) are 351bp, 536bp, 300bp and 250bp. The VP7 of the BTV, the EHDV, the VSV and the AKV and a conservative fragment of an N gene are respectively selected as targets, and the primer Express software and the primer prere 5.0 software are applied to deign and combine a primer. An optimal matching and screening test and a multi-RT-PCR test are carried out on a plurality of pairs of designed primers, and four pairs of primers which can carry out the distinguishing and detection on the four animal insect-borne diseases and have high amplification efficiency and good specificity can be obtained by a plurality of reaction condition optimization and comparison tests and verification tests. A kit formed by the reagent can obtain a qualitative distinguishing and detecting result in six hours after a sample is received. The multi-RT-PCR distinguishing and detecting reagent is a sensitive and reliable method for detecting the BTV, the EHDV, the VSV and the AKV in the clinical sample.

The invention discloses a clonorchiasis sinensis and angiostrongyliasis cantonensis real-time fluorescence PCR (Polymerase Chain Reaction) detection reagent, and a kit and a detection method thereof, wherein the detection reagent comprises two groups of compositions taking conserved segments of clonorchiasis sinensis ITS1 gene and angiostrongyliasis cantonensis ITS2 gene as targets, and the two groups of compositions respectively comprise a pair of specific primers, a specific probe and an internal standard probe. The kit comprises PCR mixed solution, Taq DNA polymerase, DEPC-H2O, a positive quality control product, a negative quality control product and pseudovirus internal standard solution; the PCR mixed solution contains the detection reagent. The detection method comprises the steps of total DNA extraction, reaction composition preparation, amplification and result judgment. The reagent and the kit are strong in specificity, high in sensitivity, pollution-free, high-throughput, and capable of accurately detecting parasite larvae, invisible to the human eyes, in food, and the detection method is fast, specific and sensitive and capable of simultaneously detecting a great amount of samples.

The related bio-reagent is designed by: with conservative West Nile virus E gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 71bp.

The invention discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) detection reagent, a kit and a detection method for an African swine fever virus (ASFV). The detection reagent is targeted to a conserved segment of an ASFV VP72 gene, and comprises a pair of specific primers, a specific probe and an internal labeling probe. The kit comprises a PCR mixed solution, Taq DNA polymerase, DEPC (diethylpyrocarbonate)-H2O, an ASFV positive quality product, an ASFV negative quality product, a quantification standard substance and a pseudovirus internal labeling solution, wherein the PCR mixed solution comprises a pair of specific primers, a specific probe and an internal labeling probe. The detection method comprises the steps of extraction of total DNAs, preparation of reaction components, making of a standard curve by diluting the standard substance, amplifying and result judgment. The reagent and the kit have the advantages of high specificity, high sensitivity, no pollution and high flux, and can be used for accurately detecting infection, inapparent infection or continuous carrying of a toxic host of low-content ASFV. The detection method is quick, specific and sensitive, and can be used for quantitatively detecting a large quantity of samples at the same time.

The invention provides a detection method for a newcastle disease virus. The method comprises operating steps of performing homology comparison by selecting a nucleoprotein (NP) gene sequence of the newcastle disease virus in an available DNA sequence database (GENEBANK), and designing primers and probes corresponding to each gene segment according to a conserved domain; synthesizing and modifying the primers and the probes, coupling the specificity probes with fluorescent-encoded microspheres to prepare specificity detection microspheres (namely a liquid chip). The liquid chip can perform specific recognition of a PCR amplification product of each of the gene segments and detection results can be read from a liquid chip detector. The detection method has advantages of high sensitivity, strong specificity, high repeatability, simple operation, short consumed time, relatively low cost, using safety, and the like.

The related bio-reagent is designed by: with conservative African horse sickness virus VP7 gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 69bp.

The invention relates to the technical field of biology, in particular to a reagent which can simultaneously distinguish and detect four animal insect-borne diseases as well as a preparation method and application of the reagent. The animal insect-borne disease multi-RT-PCR distinguishing and detecting reagent comprises four pairs of specific primers, and the respective amplification target fragment lengths of Bluetongue virus (BTV), epizootic haemorrhagic disease virus of deer (EHDV), Vesicular stomatitis virus (VSV) and Akabane virus (AKV) are 351bp, 536bp, 300bp and 250bp. The VP7 of the BTV, the EHDV, the VSV and the AKV and a conservative fragment of an N gene are respectively selected as targets, and the primer Express software and the primer prere 5.0 software are applied to deign and combine a primer. An optimal matching and screening test and a multi-RT-PCR test are carried out on a plurality of pairs of designed primers, and four pairs of primers which can carry out the distinguishing and detection on the four animal insect-borne diseases and have high amplification efficiency and good specificity can be obtained by a plurality of reaction condition optimization and comparison tests and verification tests. A kit formed by the reagent can obtain a qualitative distinguishing and detecting result in six hours after a sample is received. The multi-RT-PCR distinguishing and detecting reagent is a sensitive and reliable method for detecting the BTV, the EHDV, the VSV and the AKV in the clinical sample.

The related biological agent designed with target object as virus specific and conservative gene fragment of SVDV, VSV and FMDV, special the conservative fragment of VP1, N gene and 3D gene. Wherein, the RT-PCR detection agent includes three couple specific primers, and the amplification target fragment length is 126bp, 301bp and 189bp respectively. This invention can detect three viruses (SVDV, VSV and FMDV) in one reaction tube simultaneously.

The invention provides an automatic interpretation tool for a hemagglutination and hemagglutination inhibition test result. The tool is a device for automatically generating the result. An interpretation method for the tool comprises the following steps: adjusting a hemagglutination plate support to make erythrocytes form a teardrop shape; carrying out zoning photographing on hemagglutination plate sample holes in a fixed object distance, calculating the teardrop length of the erythrocytes, and finding out a target hole; and processing obtained results, storing original images and interpretation results in a database, carrying out classified statistics according to the test results of a sample and test items, gathering the sample, and outputting the results by clicking a menu. The hemagglutination and hemagglutination inhibition test interpretation device and the statistical method thereof allow 96 samples to be simultaneously interpreted in 4 seconds, so the interpretation speed is much faster than that of manual operation, and the time and the labor are saved. Manual analysis and discrimination are not needed by using the tool, so visual fatigue and misjudgment are avoided, and individual differences are avoided; and the possibility of personnel in contact with pathogens is reduced, and the tool can be connected with a sample information management system to realize automaticmanagement.

The invention discloses a tissue-engineered peripheral nerve tissue and a preparation method thereof, and belongs to the technical field of tissue engineering. The preparation method comprises the following steps of: separating, culturing, purifying and amplifying seed cells, inducing the seed cells in vitro to differentiate into Schwann cells, then preparing a stem cell polymer, and implanting the stem cell polymer into a silk fibroin catheter scaffold material to form the tissue-engineered peripheral nerve tissue. The tissue-engineered peripheral nerve tissue prepared by the method can be used for defect regeneration and repair of peripheral nerve tissues such as facial nerves and trigeminal branches. Compared with other types of tissue-engineered nerves existing at present, the tissue-engineered peripheral nerve tissue has the advantages that the seed cells are easy to obtain, the wound is small, the Schwann cells induced and differentiated in vitro are secreted into nerve-related factors to improve the nerve regeneration efficiency, the stem cell polymer contains sufficient cell quantity and rich extracellular matrix, and meanwhile, the tissue-engineered peripheral nerve tissue has better biological activity and good biological safety, and can significantly shorten the nerve repair time.

The related bio-reagent is designed by: with conservative African horse sickness virus VP7 gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 69bp.

The invention discloses a fluorescent quantitation RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection reagent of a West Nile virus, a kit and a detection method of the West Nile virus. The detection reagent takes a conserved fragment of a West Nile virus E gene as a target, and comprises a pair of specific primers, a specific probe and an internal labeling probe. The kit comprises PCR mixed liquor, Taq DNA (Deoxyribonucleic Acid) polymerase, DEPC-H2O, a positive quality control, a negative quality control, a quantification standard and a pseudovirus internal labeling solution, wherein the PCR mixed liquor comprises a pair of specific primers, a specific probe and an internal labeling probe. The detection method comprises extraction of total RNAs (Ribonucleic Acids), preparation of reaction components, dilution of the standard for making a standard curve, amplification and result judgment. The reagent and the kit are high in specificity, sensitivity and flux and environmental friendly, and can be used for accurately detecting low-content West Nile virus infection, unapparent infection or continuous toxic hosts. The detection method is quick, specific, sensitive and quantitative, and can be used for detecting a large number of samples at the same time.

The invention provides a polypeptide for promoting a pig body to generate the African swine fever virus antigen specific immune response, and an application of the polypeptide, and belongs to the technical field of biological medicines. The polypeptide comprises one or more of a first polypeptide, a second polypeptide, a third polypeptide, a fourth polypeptide and a fifth polypeptide, wherein amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are shown as SEQ ID NO.1-SEQ ID NO.5 respectively. The polypeptide provided by the invention can significantly promote the proliferation of ASFV sensitized immune cells, and can promote the pig body to generate the ASFV antigen specific immune response by promoting pig CD8<+>T cells to secrete IFN-gamma. After animals are immunized, the immune response of the pig body can be remarkably promoted. The polypeptide or a polypeptide polymer obtained by polypeptide polymerization can be used for preparing subunit vaccines of African swine fever virus.

The invention relates to a colloidal gold test paper strip for carrying out the quick detection of the O-shaped foot and mouth disease antibody level of artiodactyls, the preparing method of the test paper strip and a specific detection method. A sample application absorption cushion made of porous fiber material, an immune gold label layer which is arranged on the tail end of the sample application absorption cushion and is connected with the sample application absorption cushion, a detection section made of nitrocellulose film, and an absorption section which is made of water absorption material and is arranged on the tail end of the detection section and is connected with the detection section are fixed on the base plate of the test paper strip. A quality control line is an O-shaped foot and mouth disease resistant rabbit or guineapig IgG antibody, a detection line is coated with O-shaped foot and mouth disease virus, and the O-shaped foot and mouth disease virus of the detection line of the detection section and the colloidal gold label attached to the immune gold label layer is natural virus, or DNA recombination expression foot and mouth disease virus, or the decomposed antigen of the foot and mouth disease.

The related bio-reagent is designed by: with conservative African swine fever virus VP73 gene fragment as target object, designing and composing carefully for large quantities of primers and probes, taking optimal pairing screen test on different conditions to obtain the most proper primer and probe. The fluorescence quantitative RT-PCR testing reagent includes one couple of specific primers and one specific fluorescent probe, and the amplification fragment length is 66bp. This invention is fit to fast testing for African swine fever.

The present invention provides a class of polypeptides for promoting African swine fever virus antigen-specific immune responses in pigs and their application, belonging to the technical field of biomedicine; the polypeptides include a first polypeptide, a second polypeptide, a third polypeptide, One or more of the fourth polypeptide and the fifth polypeptide; the amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are respectively shown in SEQ ID Shown in NO.1~SEQ ID NO.5. The polypeptide of the present invention can significantly promote the proliferation of ASFV-sensitized immune cells, and can promote porcine CD8 + T cells secrete IFN-γ to promote ASFV antigen-specific immune response in pigs. After immunizing animals, it can significantly promote the immune response of pigs. The polypeptide of the present invention or the polypeptide polymer obtained by polymerizing the polypeptide can be used to prepare a subunit vaccine of African swine fever virus.

The invention relates to a multifunctional egg powder used for creep feed and a production method thereof, which is characterized in that the multifunctional egg powder is composed of the following components in parts by weight: 62 to 68 parts of egg powder, soybean protein isolate 24~30 parts, 3~8 parts of maltodextrin, 2~5 parts of L-lysine, 0.05~0.2 parts of lysozyme, 0.05~0.2 parts of immunoglobulin, mixed well; among them: egg powder is calculated by weight percentage , is composed of the following components: chicken whole egg liquid 50-90%, full-fat soybean powder 5-20%, soybean oil 5-30%, after stirring evenly, it is homogenized under high pressure. The homogenization pressure is 5-30MPa. The temperature is 55~70℃, heat preservation for 3~10min to sterilize, and the egg powder is quickly dried by spraying. The multifunctional egg powder contains high protein, fat, lecithin, cephalin and other functional ingredients, which can provide piglets with easy digestion and utilization. It can also add lysozyme and immunoglobulin components extracted from egg whites, which can effectively replace the use of antibiotics in suckling pig creep feed, avoid homologous transmission of plasma protein powder, and improve animal growth.

The invention provides polypeptides for promoting a swine body to generate an African swine fever virus (ASFV) antigen-specific immune response and application of the polypeptides, and belongs to thetechnical field of biological medicines. The polypeptides comprises one or more of a first polypeptide, a second polypeptide, a third polypeptide, a fourth polypeptide and a fifth polypeptide; the amino acid sequence of the first polypeptide, the amino acid sequence of the second polypeptide, the amino acid sequence of the third polypeptide, the amino acid sequence of the fourth polypeptide and the amino acid sequence of the fifth polypeptide are as shown by SEQ ID NO.1-SEQ ID NO.5 respectively. The polypeptides provided by the invention can obviously promote the proliferation of ASFV sensitized immune cells, and can promote the swine body to generate the ASFV antigen-specific immune response by promoting swine mononuclear macrophages and B and T lymphocytes to secrete interferon-gamma (IFN-gamma). After an animal is immunized, the immune response of the swine pig body can be remarkably promoted. The polypeptides or a polypeptide polymer obtained by polymerization of the polypeptides can be used for preparing subunit vaccines of the African swine fever virus.

The present invention provides a class of polypeptides for promoting African swine fever virus antigen-specific immune response in pigs and their applications, which belong to the technical field of biomedicine; the polypeptides include a first polypeptide, a second polypeptide, a third polypeptide, One or more of the fourth polypeptide and the fifth polypeptide; the amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are respectively shown in SEQ ID Shown in NO.1~SEQ ID NO.5. The polypeptide of the present invention can significantly promote the proliferation of ASFV-sensitized immune cells, and can promote the production of ASFV antigen-specific immune response by pigs by promoting the secretion of IFN-γ by pig mononuclear macrophages and B and T lymphocytes. After immunizing animals, it can significantly promote the immune response of pigs. The polypeptide of the present invention or the polypeptide polymer obtained by polymerizing the polypeptide can be used to prepare a subunit vaccine of African swine fever virus.

The invention discloses a blood layered identification method, which comprises the steps of: calibrating a sample tube; making a calibration every other set unit volume, recording physical heights of the calibrations and corresponding positions in an image; identifying boundary of blood in the sample tube; and executing a volume calculation step including the processes of calculating volume by adopting a piecewise interpolation algorithm, conducting interpolating calculation when calculating a result according to intervals to which pixel positions belong, acquiring the volume V1 and the pixel position P1 of a first calibration point as well as the volume V2 and the pixel position P2 of a second calibration point from the calibration data, and calculating V3 according to similar triangles when the pixel position of a point to be calculated is P3. The blood layered identification method can utilize 2 or even 4 cameras for shooting 16 samples at one time, and the speed is dozens of times faster than that of laser scanning. By adopting the blood layered identification method, biological safety hazards do not exist, each sample has a picture, and the operator can find out interference easily when encountering the interference.

A kind of erythrocyte sedimentation rate standard substance and preparation method thereof, including imitation human erythrocytes, protective agent (A liquid) and preservative (B liquid) placed in Wei's erythrocyte sedimentation tube, the preparation method is as follows; Step1, take the tested The fresh blood of livestock qualified for quarantine is anticoagulated with 6:1 EDTA, and the supernatant is removed after centrifugation for 5 minutes; Step2, the bottom cells after centrifugation are washed with solution A, and then centrifuged again, and the bottom red blood cells are retained; Step3, the The reserved red blood cells in the lower layer were added with 20 times of B solution and mixed thoroughly for 1 hour, and the supernatant was removed after standing for 24 hours; Step4, and then the collected and prepared mixture was mixed in A solution at a ratio of 1:1 to make the imitation Human red blood cell granules, through the flow cytometer or hematology analyzer, calculate the exact number of cells; Step5, inject the human red blood cell granules and sodium citrate in different proportions and concentrations in a 1:1 mixture At the scale inside the Weisser's ESR tube, let it stand at room temperature for 1 hour, and then seal it for storage.