Fluorescence quantificational RT-PCR detection reagent for bubble cell stomatitis virus, and preparation method

Abstract

The invention relates to a biological agent designed and synthesized by taking the gene fragment of vesicular stomatitis virus as a target, especially a reagent capable of detecting vesicular stomatitis virus and a preparation method of the reagent. The invention selects the conserved segment of the N gene of vesicular stomatitis virus as the target, and uses primer Express software and primer prere5.0 software to design and synthesize primers and probes. The most suitable primers and probes were obtained by performing optimal pairing screening experiments on the designed multiple pairs of primers and probes. The vesicular stomatitis virus fluorescent quantitative RT-PCR detection reagent of the present invention comprises a pair of specific primers and a specific fluorescent probe, and the length of the amplified target fragment is 97 bp.

Description

technical field [0001] The invention relates to a biological agent designed and synthesized by taking the gene fragment of vesicular stomatitis virus as a target, especially a reagent capable of detecting vesicular stomatitis virus and a preparation method of the reagent. Background technique [0002] Vesicular stomatitis virus (VSV) is a virus belonging to the genus Vesicularvirus in the family Rhabdoviridae. Divided into two serotypes: New Jersey type (VSV-NJ) and Indiana type (VSV-IND). It is an endemic and sporadic disease of cattle, pigs, horses, and many other mammals. People are susceptible to this virus, which can produce flu-like symptoms and blisters in the mouth, hands and feet. Infection of cattle, pigs, and horses can cause severe blisters and erosions in the oral mucosa, nipple skin, and hoof crown skin, resulting in huge economic losses. The clinical symptoms are very similar to foot-and-mouth disease (FMD), vesicular disease (SVD) and vesicular eruption (V...

AI Technical Summary

Problems solved by technology

Conventional diagnostic methods, such as virus isolation, serological tests, animal tests, etc., are time-consuming and labor-intensive, or have low specificity and sensitivity, and cannot meet the requirements of rapid and accurate diagnosis

RT-PCR can be used for the diagnosis of VS, but it also has disadvantages such as time-consuming, easy to pollute, electrophoresis detection after amplification, and small number of samples for each detection.